ID Q8E0S9_STRA5 Unreviewed; 554 AA.
AC Q8E0S9;
DT 01-MAR-2003, integrated into UniProtKB/TrEMBL.
DT 01-MAR-2003, sequence version 1.
DT 18-JUN-2025, entry version 125.
DE SubName: Full=Cell wall surface anchor family protein {ECO:0000313|EMBL:AAM99537.1};
GN OrderedLocusNames=SAG0645 {ECO:0000313|EMBL:AAM99537.1};
OS Streptococcus agalactiae serotype V (strain ATCC BAA-611 / 2603 V/R).
OC Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
OC Streptococcus.
OX NCBI_TaxID=208435 {ECO:0000313|EMBL:AAM99537.1, ECO:0000313|Proteomes:UP000000821};
RN [1] {ECO:0000313|EMBL:AAM99537.1, ECO:0000313|Proteomes:UP000000821}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-611 / 2603 V/R {ECO:0000313|Proteomes:UP000000821};
RX PubMed=12200547; DOI=10.1073/pnas.182380799;
RA Tettelin H., Masignani V., Cieslewicz M.J., Eisen J.A., Peterson S.,
RA Wessels M.R., Paulsen I.T., Nelson K.E., Margarit I., Read T.D.,
RA Madoff L.C., Wolf A.M., Beanan M.J., Brinkac L.M., Daugherty S.C.,
RA DeBoy R.T., Durkin S., Kolonay J.F., Umayam L.A., Madupu R., Lewis M.R.,
RA Radune D., Fedorova N.B., Scanlan D., Khouri H., Mulligan S., Carty H.A.,
RA Cline R.T., Gill J., Scarselli M., Mora M., Iacobini E.T., Brettoni C.,
RA Galli G., Mariani M., Vegni F., Maione D., Rinaudo D., Rappuoli R.,
RA Telford J.L., Kasper D.L., Grandi G., Fraser C.M.;
RT "Complete genome sequence and comparative genomic analysis of an emerging
RT human pathogen, serotype V Streptococcus agalactiae.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:12391-12396(2002).
RN [2] {ECO:0007829|PDB:3PF2, ECO:0007829|PDB:3PG2}
RP X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) OF 200-518 IN COMPLEX WITH CA(2+).
RX PubMed=21333654; DOI=10.1016/j.jmb.2011.02.024;
RA Vengadesan K., Ma X., Dwivedi P., Ton-That H., Narayana S.V.;
RT "A model for group B Streptococcus pilus type 1: the structure of a 35-kDa
RT C-terminal fragment of the major pilin GBS80.";
RL J. Mol. Biol. 407:731-743(2011).
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DR EMBL; AE009948; AAM99537.1; -; Genomic_DNA.
DR RefSeq; NP_687665.1; NC_004116.1.
DR RefSeq; WP_000777402.1; NC_004116.1.
DR PDB; 3PF2; X-ray; 1.70 A; A=200-518.
DR PDB; 3PG2; X-ray; 1.80 A; A/B=200-518.
DR PDBsum; 3PF2; -.
DR PDBsum; 3PG2; -.
DR AlphaFoldDB; Q8E0S9; -.
DR SMR; Q8E0S9; -.
DR STRING; 208435.SAG0645; -.
DR GeneID; 66885554; -.
DR KEGG; sag:SAG0645; -.
DR PATRIC; fig|208435.3.peg.647; -.
DR HOGENOM; CLU_029024_1_1_9; -.
DR OrthoDB; 2249722at2; -.
DR EvolutionaryTrace; Q8E0S9; -.
DR Proteomes; UP000000821; Chromosome.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR Gene3D; 2.60.40.740; -; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 2.
DR InterPro; IPR026466; Fim_isopep_form_D2_dom.
DR InterPro; IPR048052; FM1-like.
DR InterPro; IPR032334; GramPos_pilinBB.
DR InterPro; IPR032364; GramPos_pilinD1_N.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR019931; LPXTG_anchor.
DR InterPro; IPR041033; SpaA_PFL_dom_1.
DR NCBIfam; NF033902; iso_D2_wall_anc; 1.
DR NCBIfam; TIGR01167; LPXTG_anchor; 1.
DR NCBIfam; TIGR04226; RrgB_K2N_iso_D2; 1.
DR Pfam; PF00746; Gram_pos_anchor; 1.
DR Pfam; PF16569; GramPos_pilinBB; 1.
DR Pfam; PF16555; GramPos_pilinD1; 1.
DR Pfam; PF17802; SpaA; 1.
PE 1: Evidence at protein level;
KW 3D-structure {ECO:0007829|PDB:3PF2, ECO:0007829|PDB:3PG2};
KW Calcium {ECO:0007829|PDB:3PF2, ECO:0007829|PDB:3PG2};
KW Cell wall {ECO:0000256|ARBA:ARBA00022512};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Metal-binding {ECO:0007829|PDB:3PF2, ECO:0007829|PDB:3PG2};
KW Peptidoglycan-anchor {ECO:0000256|ARBA:ARBA00023088};
KW Reference proteome {ECO:0000313|Proteomes:UP000000821};
KW Secreted {ECO:0000256|ARBA:ARBA00022525};
KW Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|SAM:SignalP};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT SIGNAL 1..22
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 23..554
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5038497120"
FT TRANSMEM 525..544
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 46..201
FT /note="Gram-positive pilin subunit D1 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF16555"
FT DOMAIN 224..361
FT /note="Gram-positive pilin backbone subunit 2 Cna-B-like"
FT /evidence="ECO:0000259|Pfam:PF16569"
FT DOMAIN 385..495
FT /note="SpaA-like prealbumin fold"
FT /evidence="ECO:0000259|Pfam:PF17802"
FT DOMAIN 514..550
FT /note="Gram-positive cocci surface proteins LPxTG"
FT /evidence="ECO:0000259|Pfam:PF00746"
FT REGION 358..391
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 209
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0007829|PDB:3PF2, ECO:0007829|PDB:3PG2"
FT BINDING 210
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0007829|PDB:3PF2, ECO:0007829|PDB:3PG2"
FT BINDING 211
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0007829|PDB:3PF2, ECO:0007829|PDB:3PG2"
FT BINDING 218
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0007829|PDB:3PF2, ECO:0007829|PDB:3PG2"
FT BINDING 244
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0007829|PDB:3PF2, ECO:0007829|PDB:3PG2"
FT BINDING 358
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0007829|PDB:3PF2, ECO:0007829|PDB:3PG2"
FT BINDING 360
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0007829|PDB:3PF2, ECO:0007829|PDB:3PG2"
FT BINDING 362
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0007829|PDB:3PF2, ECO:0007829|PDB:3PG2"
FT BINDING 364
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0007829|PDB:3PF2, ECO:0007829|PDB:3PG2"
FT BINDING 366
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0007829|PDB:3PF2, ECO:0007829|PDB:3PG2"
SQ SEQUENCE 554 AA; 60168 MW; 64498D4B49729AD1 CRC64;
MKLSKKLLFS AAVLTMVAGS TVEPVAQFAT GMSIVRAAEV SQERPAKTTV NIYKLQADSY
KSEITSNGGI ENKDGEVISN YAKLGDNVKG LQGVQFKRYK VKTDISVDEL KKLTTVEAAD
AKVGTILEEG VSLPQKTNAQ GLVVDALDSK SNVRYLYVED LKNSPSNITK AYAVPFVLEL
PVANSTGTGF LSEINIYPKN VVTDEPKTDK DVKKLGQDDA GYTIGEEFKW FLKSTIPANL
GDYEKFEITD KFADGLTYKS VGKIKIGSKT LNRDEHYTID EPTVDNQNTL KITFKPEKFK
EIAELLKGMT LVKNQDALDK ATANTDDAAF LEIPVASTIN EKAVLGKAIE NTFELQYDHT
PDKADNPKPS NPPRKPEVHT GGKRFVKKDS TETQTLGGAE FDLLASDGTA VKWTDALIKA
NTNKNYIAGE AVTGQPIKLK SHTDGTFEIK GLAYAVDANA EGTAVTYKLK ETKAPEGYVI
PDKEIEFTVS QTSYNTKPTD ITVDSADATP DTIKNNKRPS IPNTGGIGTA IFVAIGAAVM
AFAVKGMKRR TKDN
//
