ID S2E6B1_9ARCH Unreviewed; 404 AA.
AC S2E6B1;
DT 18-SEP-2013, integrated into UniProtKB/TrEMBL.
DT 18-SEP-2013, sequence version 1.
DT 28-JAN-2026, entry version 32.
DE RecName: Full=DNA polymerase II large subunit {ECO:0000256|ARBA:ARBA00026137};
GN ORFNames=BG20_I1500 {ECO:0000313|EMBL:EPA06283.1};
OS Candidatus Nitrosarchaeum limnium BG20.
OC Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
OC Nitrosopumilaceae; Nitrosarchaeum.
OX NCBI_TaxID=859192 {ECO:0000313|EMBL:EPA06283.1, ECO:0000313|Proteomes:UP000014065};
RN [1] {ECO:0000313|EMBL:EPA06283.1, ECO:0000313|Proteomes:UP000014065}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BG20 {ECO:0000313|EMBL:EPA06283.1,
RC ECO:0000313|Proteomes:UP000014065};
RX PubMed=22461554; DOI=10.1128/JB.00007-12;
RA Mosier A.C., Allen E.E., Kim M., Ferriera S., Francis C.A.;
RT "Genome Sequence of "Candidatus Nitrosoarchaeum limnia" BG20, a Low-
RT Salinity Ammonia-Oxidizing Archaeon from the San Francisco Bay Estuary.";
RL J. Bacteriol. 194:2119-2120(2012).
CC -!- FUNCTION: Possesses two activities: a DNA synthesis (polymerase) and an
CC exonucleolytic activity that degrades single-stranded DNA in the 3'- to
CC 5'-direction. Has a template-primer preference which is characteristic
CC of a replicative DNA polymerase. {ECO:0000256|ARBA:ARBA00025068}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) +
CC diphosphate; Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339, Rhea:RHEA-
CC COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560, ChEBI:CHEBI:173112;
CC EC=2.7.7.7; Evidence={ECO:0000256|ARBA:ARBA00049244};
CC -!- SUBUNIT: Heterodimer of a large subunit and a small subunit.
CC {ECO:0000256|ARBA:ARBA00011315}.
CC -!- SIMILARITY: Belongs to the archaeal DNA polymerase II family.
CC {ECO:0000256|ARBA:ARBA00011053}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EPA06283.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AHJG01000077; EPA06283.1; -; Genomic_DNA.
DR RefSeq; WP_250635818.1; NZ_AHJG01000077.1.
DR AlphaFoldDB; S2E6B1; -.
DR PATRIC; fig|859192.6.peg.537; -.
DR Proteomes; UP000014065; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0004527; F:exonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR InterPro; IPR004475; PolC_DP2.
DR InterPro; IPR056171; PolC_DP2_central_dom.
DR InterPro; IPR016033; PolC_DP2_N.
DR PANTHER; PTHR42210; DNA POLYMERASE II LARGE SUBUNIT; 1.
DR PANTHER; PTHR42210:SF1; DNA POLYMERASE II LARGE SUBUNIT; 1.
DR Pfam; PF24844; PolC_DP2_central; 1.
DR Pfam; PF03833; PolC_DP2_N; 1.
PE 3: Inferred from homology;
KW DNA replication {ECO:0000256|ARBA:ARBA00022705};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Reference proteome {ECO:0000313|Proteomes:UP000014065};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 21..292
FT /note="DNA polymerase II large subunit DP2 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF03833"
FT DOMAIN 304..402
FT /note="DNA polymerase II large subunit DP2 central"
FT /evidence="ECO:0000259|Pfam:PF24844"
SQ SEQUENCE 404 AA; 44403 MW; 5713D7E375C8E114 CRC64;
MSENDAISRI SSIKMPDYYL DYYSNLSKDT YTIFEHAAFA KSTLVDSSGI IEPKIAFDLA
DRVAKMHDID IADPLRELLR IHGKELSALI ISKEIALGKY LLADATLQQK LDLAVRVGLA
IVTEGVTIAP LQGISEVTIK KNKDGSDYLS VSIAGPMRSA GGTESAVTIL IADHVRKAVG
LSKYQANCFD DETGRFVEEL RIYEREASSF QFHILDEDIE RVIANLPVEL DGVDTDPFEV
VNHKGMTRIK TDRVRGGALR VLNDGLIGRS KKLLKRIELY QLDGWEWLGD LKGAIQTGDN
QEDAAAKRMR EVITGRSVLS MPNRLGGFRL RYGRSCNTGF AAVGIHPVIA EILDHTIAVG
TQIKIDIPGK GATVAFVDSI DTPTVRLNNG DVVKIKNVKH GIEN
//
