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Database: UniProt
Entry: TCPF_VIBCH
LinkDB: TCPF_VIBCH
Original site: TCPF_VIBCH 
ID   TCPF_VIBCH              Reviewed;         338 AA.
AC   P0C6Q5; P29488; Q9KTQ9;
DT   18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT   18-MAR-2008, sequence version 1.
DT   03-AUG-2022, entry version 50.
DE   RecName: Full=Toxin coregulated pilus biosynthesis protein F;
DE   AltName: Full=TCP pilus biosynthesis protein TcpF;
DE   Flags: Precursor;
GN   Name=tcpF; OrderedLocusNames=VC_0837;
OS   Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=243277;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1400247; DOI=10.1128/jb.174.21.6974-6980.1992;
RA   Higgins D.E., Nazareno E., DiRita V.J.;
RT   "The virulence gene activator ToxT from Vibrio cholerae is a member of the
RT   AraC family of transcriptional activators.";
RL   J. Bacteriol. 174:6974-6980(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Classical Inaba Z17561 / Serotype O1;
RX   PubMed=8097178; DOI=10.1016/0378-1119(93)90589-u;
RA   Ogierman M.A., Zabihi S., Mourtzios L., Manning P.A.;
RT   "Genetic organization and sequence of the promoter-distal region of the tcp
RT   gene cluster of Vibrio cholerae.";
RL   Gene 126:51-60(1993).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8097177; DOI=10.1016/0378-1119(93)90588-t;
RA   Kaufman M.R., Shaw C.E., Jones I.D., Taylor R.K.;
RT   "Biogenesis and regulation of the Vibrio cholerae toxin-coregulated pilus:
RT   analogies to other virulence factor secretory systems.";
RL   Gene 126:43-49(1993).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39315 / El Tor Inaba N16961;
RX   PubMed=10952301; DOI=10.1038/35020000;
RA   Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L.,
RA   Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R.,
RA   Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D.,
RA   Vamathevan J.J., Bass S., Qin H., Dragoi I., Sellers P., McDonald L.A.,
RA   Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L.,
RA   Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M.;
RT   "DNA sequence of both chromosomes of the cholera pathogen Vibrio
RT   cholerae.";
RL   Nature 406:477-483(2000).
CC   -!- FUNCTION: Involved in TCP pilus biogenesis. May be a channel protein.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000305}; Peripheral
CC       membrane protein {ECO:0000305}.
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DR   EMBL; L01623; AAA27566.1; -; Genomic_DNA.
DR   EMBL; X64098; CAA45464.1; -; Genomic_DNA.
DR   EMBL; M93963; AAA27564.1; -; Genomic_DNA.
DR   EMBL; AE003852; AAF94000.1; -; Genomic_DNA.
DR   PIR; JN0526; JN0526.
DR   RefSeq; NP_230485.1; NC_002505.1.
DR   PDB; 3OC5; X-ray; 2.40 A; A=21-338.
DR   PDB; 3OC8; X-ray; 2.10 A; A=206-338.
DR   PDBsum; 3OC5; -.
DR   PDBsum; 3OC8; -.
DR   AlphaFoldDB; P0C6Q5; -.
DR   SMR; P0C6Q5; -.
DR   STRING; 243277.VC_0837; -.
DR   PRIDE; P0C6Q5; -.
DR   DNASU; 2614504; -.
DR   EnsemblBacteria; AAF94000; AAF94000; VC_0837.
DR   KEGG; vch:VC_0837; -.
DR   PATRIC; fig|243277.26.peg.798; -.
DR   HOGENOM; CLU_839222_0_0_6; -.
DR   OMA; KPYQPSD; -.
DR   BioCyc; VCHO:VC0837-MON; -.
DR   EvolutionaryTrace; P0C6Q5; -.
DR   Proteomes; UP000000584; Chromosome 1.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   Gene3D; 2.60.40.3240; -; 1.
DR   InterPro; IPR009405; TcpF.
DR   InterPro; IPR043125; TcpF_C.
DR   Pfam; PF06340; TcpF; 1.
DR   PIRSF; PIRSF020763; TcpF; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cell outer membrane; Membrane; Reference proteome; Signal.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   CHAIN           21..338
FT                   /note="Toxin coregulated pilus biosynthesis protein F"
FT                   /id="PRO_0000022477"
FT   CONFLICT        21..23
FT                   /note="FND -> INE (in Ref. 2; CAA45464)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        40
FT                   /note="D -> E (in Ref. 2; CAA45464)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        236
FT                   /note="N -> S (in Ref. 4; AAF94000)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        281
FT                   /note="N -> G (in Ref. 4; AAF94000)"
FT                   /evidence="ECO:0000305"
FT   TURN            47..50
FT                   /evidence="ECO:0007829|PDB:3OC5"
FT   HELIX           51..56
FT                   /evidence="ECO:0007829|PDB:3OC5"
FT   HELIX           61..68
FT                   /evidence="ECO:0007829|PDB:3OC5"
FT   HELIX           78..82
FT                   /evidence="ECO:0007829|PDB:3OC5"
FT   HELIX           96..104
FT                   /evidence="ECO:0007829|PDB:3OC5"
FT   HELIX           108..114
FT                   /evidence="ECO:0007829|PDB:3OC5"
FT   HELIX           116..118
FT                   /evidence="ECO:0007829|PDB:3OC5"
FT   HELIX           132..143
FT                   /evidence="ECO:0007829|PDB:3OC5"
FT   HELIX           149..154
FT                   /evidence="ECO:0007829|PDB:3OC5"
FT   STRAND          156..164
FT                   /evidence="ECO:0007829|PDB:3OC5"
FT   STRAND          167..175
FT                   /evidence="ECO:0007829|PDB:3OC5"
FT   TURN            190..192
FT                   /evidence="ECO:0007829|PDB:3OC5"
FT   STRAND          200..204
FT                   /evidence="ECO:0007829|PDB:3OC5"
FT   STRAND          228..234
FT                   /evidence="ECO:0007829|PDB:3OC8"
FT   HELIX           235..240
FT                   /evidence="ECO:0007829|PDB:3OC8"
FT   STRAND          248..256
FT                   /evidence="ECO:0007829|PDB:3OC8"
FT   TURN            260..262
FT                   /evidence="ECO:0007829|PDB:3OC8"
FT   STRAND          269..274
FT                   /evidence="ECO:0007829|PDB:3OC8"
FT   STRAND          286..292
FT                   /evidence="ECO:0007829|PDB:3OC8"
FT   HELIX           295..297
FT                   /evidence="ECO:0007829|PDB:3OC8"
FT   HELIX           301..306
FT                   /evidence="ECO:0007829|PDB:3OC8"
FT   STRAND          313..320
FT                   /evidence="ECO:0007829|PDB:3OC8"
FT   HELIX           335..337
FT                   /evidence="ECO:0007829|PDB:3OC8"
SQ   SEQUENCE   338 AA;  38211 MW;  82D6E9B660506939 CRC64;
     MRYKKTLMLS IMITSFNSFA FNDNYSSTST VYATSNEATD SRGSEHLRYP YLECIKIGMS
     RDYLENCVKV SFPTSQDMFY DAYPSTESDG AKTRTKEDFS ARLLAGDYDS LQKLYIDFYL
     AQTTFDWEIP TRDQIETLVN YANEGKLSTA LNQEYITGRF LTKENGRYDI VNVGGVPDNT
     PVKLPAIVSK RGLMGTTSVV NAIPNEIYPH IKVYEGTLSR LKPGGAMIAV LEYDVNELSK
     HGYTNLWDVQ FKVLVGVPHA ETGVIYDPVY EETVKPYQPS NNLTGKKLYN VSTNDMHNGY
     KWSNTMFSNS NYKTQILLTK GDGSGVKLYS KAYSENFK
//
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