ID W4PYN8_9BACI Unreviewed; 383 AA.
AC W4PYN8;
DT 19-MAR-2014, integrated into UniProtKB/TrEMBL.
DT 19-MAR-2014, sequence version 1.
DT 28-JAN-2026, entry version 49.
DE SubName: Full=Radical SAM domain heme biosynthesis protein {ECO:0000313|EMBL:GAE24847.1};
GN ORFNames=JCM9140_806 {ECO:0000313|EMBL:GAE24847.1};
OS Halalkalibacter wakoensis JCM 9140.
OC Bacteria; Bacillati; Bacillota; Bacilli; Bacillales; Bacillaceae;
OC Halalkalibacter.
OX NCBI_TaxID=1236970 {ECO:0000313|EMBL:GAE24847.1, ECO:0000313|Proteomes:UP000018890};
RN [1] {ECO:0000313|EMBL:GAE24847.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JCM 9140 {ECO:0000313|EMBL:GAE24847.1};
RA Yuki M., Oshima K., Suda W., Oshida Y., Kitamura K., Iida T., Hattori M.,
RA Ohkuma M.;
RT "Draft Genome Sequences of Three Alkaliphilic Bacillus Strains, Bacillus
RT wakoensis JCM 9140T, Bacillus akibai JCM 9157T, and Bacillus
RT hemicellulosilyticus JCM 9152T.";
RL Genome Announc. 2:e01258-13(2014).
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GAE24847.1}.
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DR EMBL; BAUT01000005; GAE24847.1; -; Genomic_DNA.
DR RefSeq; WP_052002037.1; NZ_BAUT01000005.1.
DR AlphaFoldDB; W4PYN8; -.
DR STRING; 1236970.JCM9140_806; -.
DR OrthoDB; 9782387at2; -.
DR Proteomes; UP000018890; Unassembled WGS sequence.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR CDD; cd01335; Radical_SAM; 1.
DR CDD; cd21123; SPASM_MftC-like; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR InterPro; IPR023885; 4Fe4S-binding_SPASM_dom.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR017200; PqqE-like.
DR InterPro; IPR050377; Radical_SAM_PqqE_MftC-like.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR058240; rSAM_sf.
DR NCBIfam; TIGR04053; TIGR04053 family radical SAM/SPASM domain-containing protein; 1.
DR PANTHER; PTHR11228; RADICAL SAM DOMAIN PROTEIN; 1.
DR PANTHER; PTHR11228:SF34; TUNGSTEN-CONTAINING ALDEHYDE FERREDOXIN OXIDOREDUCTASE COFACTOR MODIFYING PROTEIN; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR Pfam; PF13186; SPASM; 1.
DR PIRSF; PIRSF037420; PQQ_syn_pqqE; 1.
DR SFLD; SFLDG01386; main_SPASM_domain-containing; 1.
DR SFLD; SFLDS00029; Radical_SAM; 1.
DR SFLD; SFLDG01067; SPASM/twitch_domain_containing; 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000018890};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691}.
FT DOMAIN 7..217
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 383 AA; 43868 MW; 36786C5908DB11E1 CRC64;
MFNRDFHKNP FIVIWELTRA CELKCLHCRA EAQHHRHPHE LTFDEGKNLI HSIYEMDNPM
LVFTGGDPLM RPDMFDIVDY AVKKGVRVSI TPSATPNVTK EIIQKAKEVG LSRWAFSLDG
PTASIHDHFR GTKGSFELTI KAIHYLHKLN IPVQINTVVS KYNVNVLEEM AKVVEELNCV
LWSVFFLVPI GRGSEKDMIS PAEHERVFHW LHQLNKNVTF DIKTTAAQHY RRVVIQQKMK
ETSTFDQTVR YEDALFKGIT SPFDGLGRAP KGVNDGNGFV FISHVGDVYP SGLLPVRAGN
VRETPLAEIY RESPIFKNLR NPDKYKGKCG VCEFRYICGG SRSRAYAITG DYMESEPYCV
YIPKTLRKQK SEKHRSRSTQ IHL
//
