S0: Osaka Bay Oct.
    56 pairs of primer + 26 & 38 PCR cycles of PP16 & PP45
S1: Osaka Bay Aug.
S2: Japan Sea
S3: Miyuki hot spring
S4: Ishigaki Island

References:

Li Y., Hingamp P., Watai H., Endo H., Yoshida T., Ogata H.
Degenerate PCR primers to reveal the diversity of giant viruses in coastal waters.
Viruses, 10, 496 (2018).

Yanze Li, Hisashi Endo, Yasuhiro Goto, Hiroyasu Watai, Nana Ogawa, Romain Blanc-Mathieu, Takashi Yoshida, Hiroyuki Ogata
The earth is small for leviathans: long distance dispersal of giant viruses across aquatic environments.
(manuscript in prep.)