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EGassembler

Aligns and merges sequence fragments resulting from shotgun sequencing or gene transcripts (EST) fragments in order to reconstruct the original segment or gene
Home One-Click Assembly Step-by-Step Assembly Stand-Alone Processing Help

PROJECT NAME EMAIL ADDRESS

Query sequences
TEXT DATA , Please paste your sequences (FASTA format)
FILE UPLOAD(FASTA format)

Enable Sequence Cleaning Process

Options

number of CPUs
minimum percent identity for an alignemnt with a contaminant

Enable Repeat Masking Process

Options

number of CPUs
Repeats Library
Human
Using your custom repeats library
Using EGrep repeats library (Our custom repeats library)
Using RepBase repeats library
Using TREP repeats library
Using TIGR repeats library

Enable Vector Masking Process

Options

number of CPUs
Vector Library
Using your custom vector library
Using EGvec vector library (Our custom vectors library)
Using NCBI's vector library
Using EMBL vector library

Enable Organelle Masking Process

Options

number of CPUs
Organelle Library
Using your custom organelle library
Using Plastids library
Using Mitochondria library

Enable Sequence Assembly Process

Options

overlap percent identity cutoff N > 65
Home KEGG GenomeNet Kanehisa Laboratory

Reference:
Masoudi-Nejad A, Tonomura K, Kawashima S, Moriya Y, Suzuki M, Itoh M, Kanehisa M, Endo T, Goto S (2006)
EGassembler: online bioinformatics service for large-scale processing, clustering and assembling ESTs and genomic DNA fragments. Nucleic Acids Res. 34:W459-462.