Multiple Sequence Alignment by MAFFT
ETE3
MAFFT
CLUSTALW
PRRN
Multiple sequence alignment by MAFFT v6.864
Basic settings
Input
:
Paste protein or DNA sequences in fasta format
or upload a file:
Advanced settings
Output order
:
Same as input
Aligned
Strategy
:
Moderately accurate (FFT-NS-2, FFT-NS-i or L-INS-i; depends on data size)
FFT-NS-1 (Very fast; progressive method)
FFT-NS-2 (Fast; progressive method)
(Medium; iterative refinement method, two cycles only)
FFT-NS-i (Slow; iterative refinement method)
L-INS-i (Very slow; probably most accurate; recommended for <200 sequences)
G-INS-i (Very slow; suitable for sequences of similar lengths; recommended for <200 sequences)
E-INS-i (Very slow; suitable for sequences containing large unalignable regions; recommended for <200 sequences)
Parameters
:
Scoring matrix (for amino acid sequences):
BLOSUM30
BLOSUM45
BLOSUM62
BLOSUM80
JTT100
JTT200
Gap opening penalty:
(1.0-3.0)
Offset value:
(0.0-1.0; functions like gap extension penalty)
mafftE (aligns input sequences together with their homologues collected from SwissProt by BLAST)
:
On
Show collected homologues also (if any)
Number of homologues:
(5-200)
Threshold:
(1e-5 - 1e-40)
KEGG
GenomeNet
Kyoto University Bioinformatics Center