KofamKOALA - KEGG Orthology Search
K number assignment based on KO-dependent scoring criteria
BlastKOALA
GhostKOALA
KofamKOALA
DeepKOALA
KofamKOALA assigns K numbers to the user's sequence data by HMMER/HMMSEARCH against KOfam (a customized HMM database of KEGG Orthologs (KOs)). K number assignments with scores above the predefined thresholds for individual KOs are more reliable than other proposed assignments. Such high score assignments are highlighted with asterisks '*' in the output. The K number assignments facilitate the interpretation of the annotation results by linking the user's sequence data to the KEGG pathways and EC numbers.
KOfam Search
(keyword search for IDs or Definitions)
Enter FASTA Sequences
or upload a sequence file
E-value
Hits with scores above the predefined adaptive thresholds and E-values
lower than or equal to the specified threshold will be reported with '*'.
E-mail
Current release
ver. 2026-05-01
KEGG release 118.0
Download
KOfam - HMM profiles for KEGG/KO with predefined score thresholds (download by [
FTP
][
HTTPS
])
KofamScan - Software to search KOfam (download by [
FTP
][
HTTPS
])
Reference
Aramaki T., Blanc-Mathieu R., Endo H., Ohkubo K., Kanehisa M., Goto S., Ogata H.
KofamKOALA: KEGG ortholog assignment based on profile HMM and adaptive score threshold.
Bioinformatics. 2019 Nov 19. pii: btz859. doi:
10.1093/bioinformatics/btz859
.
Last updated: November 25, 2019
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