KEGG   ENZYME: 1.1.3.47Help
Entry
EC 1.1.3.47                 Enzyme                                 

Name
5-(hydroxymethyl)furfural oxidase
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With oxygen as acceptor
BRITE hierarchy
Sysname
5-(hydroxymethyl)furfural:oxygen oxidoreductase
Reaction(IUBMB)
5-(hydroxymethyl)furfural + 3 O2 + 2 H2O = furan-2,5-dicarboxylate + 3 H2O2 (overall reaction) [RN:R10858];
(1a) 5-(hydroxymethyl)furfural + O2 = furan-2,5-dicarbaldehyde + H2O2 [RN:R10853];
(1b) furan-2,5-dicarbaldehyde + H2O = 5-(dihydroxymethyl)furan-2-carbaldehyde (spontaneous) [RN:R10854];
(1c) 5-(dihydroxymethyl)furan-2-carbaldehyde + O2 = 5-formylfuran-2-carboxylate + H2O2 [RN:R10855];
(1d) 5-formylfuran-2-carboxylate + H2O = 5-(dihydroxymethyl)furan-2-carboxylate (spontaneous) [RN:R10856];
(1e) 5-(dihydroxymethyl)furan-2-carboxylate + O2 = furan-2,5-dicarboxylate + H2O2 [RN:R10857]
Reaction(KEGG)
Substrate
5-hydroxymethylfurfural [CPD:C11101];
O2 [CPD:C00007];
H2O [CPD:C00001];
furan-2,5-dicarbaldehyde [CPD:C20899];
5-(dihydroxymethyl)furan-2-carbaldehyde [CPD:C20900];
5-formylfuran-2-carboxylate [CPD:C20449];
5-(dihydroxymethyl)furan-2-carboxylate [CPD:C20901]
Product
furan-2,5-dicarboxylate [CPD:C20450];
H2O2 [CPD:C00027];
furan-2,5-dicarbaldehyde [CPD:C20899];
5-(dihydroxymethyl)furan-2-carbaldehyde [CPD:C20900];
5-formylfuran-2-carboxylate [CPD:C20449];
5-(dihydroxymethyl)furan-2-carboxylate [CPD:C20901]
Comment
The enzyme, characterized from the bacterium Methylovorus sp. strain MP688, is involved in the degradation and detoxification of 5-(hydroxymethyl)furfural. The enzyme acts only on alcohol groups and requires the spontaneous hydration of aldehyde groups for their oxidation [3]. The enzyme has a broad substrate range that overlaps with EC 1.1.3.7, aryl-alcohol oxidase.
History
EC 1.1.3.47 created 2014
Pathway
Furfural degradation
Microbial metabolism in diverse environments
Orthology
K16873  
5-(hydroxymethyl)furfural/furfural oxidase
Genes
PSK: 
PRH: 
HAK: 
CNC: 
BCJ: 
BCEN: 
BUG: 
BGE: 
BGF: 
BGD: 
BGO: 
BYI: 
BUE: 
BUQ: 
BXE: 
BXB: 
BPH: 
BPX: 
BPY: 
BFN: 
BCAI: 
AMIM: 
MEI: 
MEP: 
MPQ_0130(betA)
ARA: 
ATF: 
RLT: 
BJA: 
BJU: 
BJP: 
BRS: 
BRC: 
BRAD: 
MRD: 
MET: 
MNO: 
MOR: 
MAQU: 
DEQ: 
RHZ: 
AUA: 
HAT: 
SWI: 
SCH: 
SRC: 
 » show all
Taxonomy
Reference
1  [PMID:20194784]
  Authors
Koopman F, Wierckx N, de Winde JH, Ruijssenaars HJ.
  Title
Identification and characterization of the furfural and 5-(hydroxymethyl)furfural degradation pathways of Cupriavidus basilensis HMF14.
  Journal
Proc. Natl. Acad. Sci. U. S. A. 107 (2010) 4919-24.
  Sequence
Reference
2  [PMID:24271187]
  Authors
Dijkman WP, Fraaije MW
  Title
Discovery and characterization of a 5-hydroxymethylfurfural oxidase from Methylovorus sp. strain MP688.
  Journal
Appl. Environ. Microbiol. 80 (2014) 1082-90.
  Sequence
[mep:MPQ_0130]
Reference
3  [PMID:24802551]
  Authors
Dijkman WP, Groothuis DE, Fraaije MW
  Title
Enzyme-catalyzed oxidation of 5-hydroxymethylfurfural to furan-2,5-dicarboxylic acid.
  Journal
Angew. Chem. Int. Ed. Engl. 53 (2014) 6515-8.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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