| Entry |
|
|---|
| Name |
3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione
4,5-dioxygenase;
steroid 4,5-dioxygenase;
3-alkylcatechol 2,3-dioxygenase |
|---|
| Class |
Oxidoreductases;
Acting on single donors with O2 as oxidant and incorporation of
oxygen into the substrate (oxygenases). The oxygen incorporated need
not be derived from O2;
With incorporation of two atoms of oxygen
 |
|---|
| Sysname |
3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione:oxygen
4,5-oxidoreductase (decyclizing) |
|---|
| Reaction(IUBMB) |
3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + O2 =
3-hydroxy-5,9,17-trioxo-4,5:9,10-disecoandrosta-1(10),2-dien-4-oate
[RN:R04597] |
|---|
| Reaction(KEGG) |
R04597
 |
|---|
| Substrate |
3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione
[CPD:C04793];
O2 [CPD:C00007] |
|---|
| Product |
3-hydroxy-5,9,17-trioxo-4,5:9,10-disecoandrosta-1(10),2-dien-4-oate
[CPD:C04844] |
|---|
| Cofactor |
Iron [CPD:C00023] |
|---|
| Comment |
Requires Fe2+. Also acts on 3-isopropylcatechol and
3-tert-butyl-5-methylcatechol. |
|---|
Reference
Authors
Title
Journal
Organism
|
1 [PMID:5908121]
Gibson DT, Wang KC, Sih CJ, Whitlock H Jr.
Mechanisms of steroid oxidation by microorganisms. IX. On the
mechanism of ring A cleavage in the degradation of 9,10-seco
steroids by microorganisms.
J. Biol. Chem. 241 (1966) 551-9.
Nacardia restrictus |
|---|
| Other DBs |
ExplorEnz - The Enzyme Database: 1.13.11.25
IUBMB Enzyme Nomenclature: 1.13.11.25
ExPASy - ENZYME nomenclature database: 1.13.11.25
UM-BBD (Biocatalysis/Biodegradation Database): 1.13.11.25
BRENDA, the Enzyme Database: 1.13.11.25
CAS: 37256-63-6 |
|---|
|
