KEGG   ENZYME: 1.13.11.63Help
Entry
EC 1.13.11.63               Enzyme                                 

Name
beta-carotene 15,15'-dioxygenase;
blh (gene name)
Class
Oxidoreductases;
Acting on single donors with incorporation of molecular oxygen (oxygenases);
With incorporation of two atoms of oxygen
BRITE hierarchy
Sysname
beta-carotene:oxygen 15,15'-dioxygenase (bond-cleaving)
Reaction(IUBMB)
beta-carotene + O2 = 2 all-trans-retinal [RN:R00032]
Reaction(KEGG)
Substrate
beta-carotene [CPD:C02094];
O2 [CPD:C00007]
Product
all-trans-retinal [CPD:C00376]
Comment
Requires Fe2+. The enzyme, which has been characterized from the uncultured marine bacterium 66A03, is involved in the proteorhodopsin system, which uses retinal as its chromophore. The enzyme cleaves beta-carotene symmetrically, producing two molecules of all-trans-retinal. Both atoms of the oxygen molecule are incorporated into the products. It requires a substrate greater than C35 that contains at least one unsubstituted beta-end group. cf. EC 1.14.99.36, beta-carotene 15,15-monooxygenase.
History
EC 1.13.11.63 created 2012
Pathway
Retinol metabolism
Metabolic pathways
Orthology
K00515  
beta-carotene 15,15'-dioxygenase
Genes
HSA: 
53630(BCO1)
PTR: 
468043(BCO1)
PPS: 
100987572(BCO1)
GGO: 
101132534(BCMO1)
PON: 
100454129(BCMO1)
NLE: 
100603806(BCO1)
MCC: 
MCF: 
102121880(BCMO1)
RRO: 
104671629(BCO1)
CJC: 
100393264(BCO1)
MMU: 
63857(Bco1)
RNO: 
114106(Bco1)
CGE: 
100760350(Bcmo1)
NGI: 
103732128(Bco1)
HGL: 
101724039(Bco1)
OCU: 
100358087(BCMO1)
TUP: 
102484616(BCMO1)
CFA: 
489695(BCMO1)
AML: 
100483748(BCO1)
UMR: 
103662084(BCO1)
FCA: 
101101606(BCO1)
PTG: 
102959940(BCMO1)
BTA: 
534696(BCO1)
BOM: 
102266127(BCMO1)
PHD: 
102315733(BCMO1)
CHX: 
102179507(BCMO1)
OAS: 
100294509(BCMO1) 101121875(BCO1)
SSC: 
100192320(BCMO1)
CFR: 
102513983(BCMO1)
BACU: 
103015035(BCMO1)
LVE: 
103072015(BCMO1)
ECB: 
100069873(BCMO1)
MYB: 
102263079(BCMO1)
MYD: 
102771959(BCMO1)
PALE: 
102897588(BCMO1)
MDO: 
100031779(BCMO1)
SHR: 
100920512(BCO1)
OAA: 
103171493(BCMO1)
GGA: 
395346(BCMO1)
MGP: 
100549225(BCO1)
APLA: 
101798614(BCO1)
TGU: 
100228904(BCO1)
GFR: 
102040064(BCMO1)
FAB: 
101812113(BCMO1)
PHI: 
102103988(BCMO1)
CCW: 
104691821(BCO1)
FPG: 
101914506(BCMO1)
FCH: 
102058462(BCMO1)
CLV: 
102086476(BCMO1)
ASN: 
102378066(BCMO1)
AMJ: 
102573601(BCMO1)
PSS: 
102455966(BCMO1)
CMY: 
102929893(BCMO1)
ACS: 
100563614(bcmo1)
XTR: 
100486582(bco1)
DRE: 
393580(bco1l) 84039(bco1)
TRU: 
MZE: 
OLA: 
XMA: 
LCM: 
102362963(BCMO1)
CMK: 
103175958(bcmo1)
BFO: 
CIN: 
778841(bco)
SPU: 
ISC: 
LGI: 
HWA: 
 » show all
Taxonomy
Reference
1  [PMID:19366683]
  Authors
Kim YS, Kim NH, Yeom SJ, Kim SW, Oh DK
  Title
In vitro characterization of a recombinant Blh protein from an uncultured marine  bacterium as a beta-carotene 15,15'-dioxygenase.
  Journal
J. Biol. Chem. 284 (2009) 15781-93.
Reference
2  [PMID:20229064]
  Authors
Kim YS, Park CS, Oh DK
  Title
Retinal production from beta-carotene by beta-carotene 15,15'-dioxygenase from an unculturable marine bacterium.
  Journal
Biotechnol. Lett. 32 (2010) 957-61.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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