KEGG   ENZYME: 1.13.11.63Help
Entry
EC 1.13.11.63               Enzyme                                 

Name
beta-carotene 15,15'-dioxygenase;
blh (gene name)
Class
Oxidoreductases;
Acting on single donors with incorporation of molecular oxygen (oxygenases);
With incorporation of two atoms of oxygen
BRITE hierarchy
Sysname
beta-carotene:oxygen 15,15'-dioxygenase (bond-cleaving)
Reaction(IUBMB)
beta-carotene + O2 = 2 all-trans-retinal [RN:R00032]
Reaction(KEGG)
Substrate
beta-carotene [CPD:C02094];
O2 [CPD:C00007]
Product
all-trans-retinal [CPD:C00376]
Comment
Requires Fe2+. The enzyme, which has been characterized from the uncultured marine bacterium 66A03, is involved in the proteorhodopsin system, which uses retinal as its chromophore. The enzyme cleaves beta-carotene symmetrically, producing two molecules of all-trans-retinal. Both atoms of the oxygen molecule are incorporated into the products. It requires a substrate greater than C35 that contains at least one unsubstituted beta-end group. cf. EC 1.14.99.36, beta-carotene 15,15-monooxygenase.
History
EC 1.13.11.63 created 2012
Reference
1  [PMID:19366683]
  Authors
Kim YS, Kim NH, Yeom SJ, Kim SW, Oh DK
  Title
In vitro characterization of a recombinant Blh protein from an uncultured marine  bacterium as a beta-carotene 15,15'-dioxygenase.
  Journal
J. Biol. Chem. 284 (2009) 15781-93.
  Organism
Uncultured marine bacterium
  Sequence
[up:Q4PNI0]
Reference
2  [PMID:20229064]
  Authors
Kim YS, Park CS, Oh DK
  Title
Retinal production from beta-carotene by beta-carotene 15,15'-dioxygenase from an unculturable marine bacterium.
  Journal
Biotechnol. Lett. 32 (2010) 957-61.
  Organism
Uncultured marine bacterium
  Sequence
[up:Q4PNI0]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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