KEGG   ENZYME: 1.13.11.79Help
Entry
EC 1.13.11.79               Enzyme                                 

Name
5,6-dimethylbenzimidazole synthase;
BluB
Class
Oxidoreductases;
Acting on single donors with incorporation of molecular oxygen (oxygenases);
With incorporation of two atoms of oxygen
BRITE hierarchy
Sysname
FMNH2 oxidoreductase (5,6-dimethylbenzimidazole-forming)
Reaction(IUBMB)
FMNH2 + O2 = 5,6-dimethylbenzimidazole + D-erythrose 4-phosphate + other product(s) [RN:R09083]
Reaction(KEGG)
Substrate
FMNH2 [CPD:C01847];
O2 [CPD:C00007]
Product
5,6-dimethylbenzimidazole [CPD:C03114];
D-erythrose 4-phosphate [CPD:C00279];
other product(s)
Comment
The enzyme catalyses a complex oxygen-dependent conversion of reduced flavin mononucleotide to form 5,6-dimethylbenzimidazole, the lower ligand of vitamin B12. This conversion involves many sequential steps in two distinct stages, and an alloxan intermediate that acts as a proton donor, a proton acceptor, and a hydride acceptor [4]. The C-2 of 5,6-dimethylbenzimidazole is derived from C-1' of the ribityl group of FMNH2 and 2-H from the ribityl 1'-pro-S hydrogen. While D-erythrose 4-phosphate has been shown to be one of the byproducts, the nature of the other product(s) has not been verified yet.
History
EC 1.13.11.79 created 2010 as EC 1.14.99.40, transferred 2014 to EC 1.13.11.79
Pathway
ec00740  Riboflavin metabolism
ec01100  Metabolic pathways
Orthology
K04719  5,6-dimethylbenzimidazole synthase
Genes
FAU: Fraau_1297
DJA: HY57_01060
VSP: VS_II1157
VNI: VIBNI_B0592(bluB)
VCY: IX92_08570
VCT: JV59_29945
PAE: PA1274
PAEV: N297_1314(bluB)
PAEI: N296_1314(bluB)
PAU: PA14_47750
PAEP: PA1S_19535
PAEM: U769_19385
PAEL: T223_20630
PAEG: AI22_14425
PAEC: M802_1311(bluB)
PAEO: M801_1313(bluB)
PMY: Pmen_1752
PMK: MDS_3110
PPSE: BN5_1585(bluB)
PCQ: PcP3B5_15460(bluB)
PPU: PP_1674(bluB)
PPF: Pput_4045
PPT: PPS_1325
PPI: YSA_02431
PPX: T1E_3648
PPUH: B479_06405
PPUT: L483_05925
PPUN: PP4_40970
PMON: X969_04500
PMOT: X970_04475
PSB: Psyr_3679
PSYR: N018_07015
PFL: PFL_4429(bluB)
PPRO: PPC_4538
PFS: PFLU_4489
PFE: PSF113_4408(bluB)
PFC: PflA506_3794(bluB)
PFW: PF1751_v1c39830(bluB)
PFB: VO64_5046
PMAN: OU5_5263
PEN: PSEEN1380
PSA: PST_1293
PSTT: CH92_07715
PPUU: PputUW4_03773(bluB)
PKC: PKB_1569
PSES: PSCI_0381
PSEM: TO66_23155
PSOS: POS17_4501(bluB)
PANR: A7J50_4169
SPL: Spea_0684
SHL: Shal_0741
SWD: Swoo_1863
SWP: swp_4403
SVO: SVI_0196
SPSW: Sps_00268
CPS: CPS_2468
MHC: MARHY0223
MBS: MRBBS_0221(bluB)
MVS: MVIS_3206
MCA: MCA2289
MAH: MEALZ_0954(bluB)
MPSY: CEK71_07165(bluB)
MEJ: Q7A_2235(bluB)
TKM: TK90_1459
TVR: TVD_07100
HCH: HCH_06460
HEL: HELO_1843
HAM: HALO1452
KAK: Kalk_03885(bluB)
OME: OLMES_4860(bluB)
OCE: GU3_05380
CVI: CV_1555
CHRO: CXB49_14740(bluB)
RSO: RSp0614
RSN: RSPO_m00962(nfnB)
RSE: F504_4611
RIN: ACS15_1510(bluB)
CNC: CNE_1c18130(bluB)
RME: Rmet_2213
BMA: BMA2051
BMAL: DM55_2403(bluB)
BMAE: DM78_2620(bluB)
BMAQ: DM76_2388(bluB)
BMAI: DM57_840
BMAF: DM51_1710(bluB)
BMAZ: BM44_1298(bluB)
BMAB: BM45_860(bluB)
BPS: BPSL2735
BPL: BURPS1106A_3208(bluB)
BPD: BURPS668_3169(bluB)
BPR: GBP346_A3349(bluB)
BPSE: BDL_2711(bluB)
BPSM: BBQ_576(bluB)
BPSU: BBN_704(bluB)
BPSD: BBX_1109(bluB)
BPK: BBK_2197(bluB)
BPSH: DR55_1783(bluB)
BPSA: BBU_2828(rfaE)
BPSO: X996_1424(bluB)
BUT: X994_3423(bluB)
BTE: BTH_I1401
BTQ: BTQ_2531(bluB)
BTJ: BTJ_3163(bluB)
BTZ: BTL_1094(bluB)
BTD: BTI_869(bluB)
BTV: BTHA_1186(bluB)
BTHE: BTN_294(bluB)
BTHM: BTRA_1302(bluB)
BTHA: DR62_460
BTHL: BG87_1291(bluB)
BOK: DM82_2401(bluB)
BOC: BG90_2332(bluB)
BVE: AK36_44(bluB)
BCN: Bcen_0341
BCJ: BCAL3180
BCEN: DM39_635(bluB)
BCEW: DM40_1490(bluB)
BCEO: I35_0677
BAM: Bamb_0704
BMU: Bmul_2559
BMK: DM80_861(bluB)
BMUL: NP80_840(bluB)
BCT: GEM_2704
BCED: DM42_1005(bluB)
BDL: AK34_2348(bluB)
BCON: NL30_11665
BUB: BW23_912(bluB)
BLAT: WK25_03835
BTEI: WS51_14400
BSEM: WJ12_03730
BPSL: WS57_21530
BMEC: WJ16_03765
BSTG: WT74_04030
BGP: BGL_1c06890(bluB)
BGU: KS03_1624(bluB)
BGO: BM43_2137(bluB)
BUK: MYA_0715
BUL: BW21_964(bluB)
BXE: Bxe_B1657
BXB: DR64_6965(bluB)
BPH: Bphy_3158
BFN: OI25_4089(bluB)
PARB: CJU94_21265(bluB)
PHS: C2L64_26605(bluB)
PTER: C2L65_21780(bluB)
RFR: Rfer_2617
POL: Bpro_2763
PNA: Pnap_2149
AAV: Aave_1544
AJS: Ajs_3177
AAA: Acav_1584
DAC: Daci_2803
VBO: CKY39_15830(bluB)
CTES: O987_11520
CBAB: SMCB_0328
MPT: Mpe_B0452(cysG) Mpe_B0487(cysG)
HAR: HEAR0425
MMS: mma_2554
HSE: Hsero_2645(nfnB)
HRB: Hrubri_2734(nfnB)
HEE: hmeg3_12380(bluB)
MASS: CR152_18195(bluB)
RGE: RGE_38910
TBD: Tbd_2717
MFA: Mfla_0115
MMB: Mmol_0273
MEH: M301_0171
MEP: MPQ_0158
EBA: ebA4029
DSU: Dsui_0051
DAR: Daro_0141
AZO: azo3524
AZA: AZKH_0971(bluB)
AOA: dqs_3667
TCL: Tchl_0969
SUA: Saut_0152
ARC: ABLL_0134
MLO: mll7468
SME: SMc00166
SMX: SM11_chr1558(bluB)
SMEL: SM2011_c00166(bluB)
SMER: DU99_10045
SMD: Smed_1562
RHI: NGR_c16920(bluB)
SFD: USDA257_c41170(bluB)
SIX: BSY16_896(bluB)
SAME: SAMCFNEI73_Ch2071(bluB)
ATU: Atu1654
ARA: Arad_3252
AVI: Avi_2042
AGC: BSY240_349(bluB)
REL: REMIM1_CH02380(bluB)
REI: IE4771_CH02490(bluB)
REP: IE4803_CH02402(bluB)
RLE: RL2681
RLG: Rleg_2211
RTR: RTCIAT899_CH12935(bluB)
RIR: BN877_I1614(bluB)
RHL: LPU83_2251(bluB)
RGA: RGR602_CH02141(bluB)
RHN: AMJ98_CH02418(bluB)
RPHA: AMC79_CH02487(bluB)
RHX: AMK02_CH02433(bluB)
SHZ: shn_09920
BME: BMEII0246
BMEL: DK63_2996(bluB)
BMI: BMEA_B1040(bluB)
BMEE: DK62_2402(bluB)
BMF: BAB2_1015
BABO: DK55_2957(bluB)
BABR: DO74_2595(bluB)
BABT: DK49_3055(bluB)
BABB: DK48_2839(bluB)
BABU: DK53_2187(bluB)
BABS: DK51_2767(bluB)
BABC: DO78_2874(bluB)
BMS: BRA1054
BMT: BSUIS_B1050(bluB)
BSZ: DK67_3001(bluB)
BCS: BCAN_B1076(bluB)
BCAR: DK60_2738(bluB)
BCAS: DA85_15545
BPV: DK65_2602(bluB)
OAN: Oant_1328
OAH: DR92_891(bluB)
OCH: CES85_0268(bluB)
BJA: bll3254
BRA: BRADO4896
BBT: BBta_3155
BRS: S23_47500
AOL: S58_27940(BluB)
BRAD: BF49_1444
BRO: BRAD285_2340(bluB)
BRK: CWS35_19560(bluB)
RPA: RPA0711
RPB: RPB_0743
RPC: RPC_3749
RPD: RPD_0641
RPE: RPE_2232
RPT: Rpal_0782
BOS: BSY19_867(bluB)
XAU: Xaut_2810
AZC: AZC_4661
SNO: Snov_0877
MEX: Mext_2003
MDI: METDI2769
MET: M446_3671
MPO: Mpop_1959
MNO: Mnod_4520
MOR: MOC_1239(bliB)
META: Y590_09565
MAQU: Maq22A_c24535(nfnB)
MEE: CS521_24095(bluB)
BID: Bind_0168
MSL: Msil_1629
RVA: Rvan_1099
FIL: BN1229_v1_1057(bluB)
FIY: BN1229_v1_1059(bluB)
BVR: BVIR_899
MSC: BN69_2844
MTW: CQW49_01800(bluB)
MCG: GL4_2686
HDI: HDIA_2853(nox)
SIL: SPO3254(bluB)
RSP: RSP_3218
RCP: RCAP_rcc02052(bluB)
RDE: RD1_3815(bluB)
RLI: RLO149_c006290(bluB)
PDE: Pden_2527
PZH: CX676_12530(bluB)
PARO: CUV01_05585(bluB)
DSH: Dshi_0347(bluB)
PSF: PSE_4445(blub)
PGD: Gal_00189
OAT: OAN307_c11000(bluB1) OAN307_c37400(bluB2)
OAR: OA238_c14410(bluB)
OTM: OSB_01690
CID: P73_0743
CEH: CEW89_16045(bluB)
MALG: MALG_01223
RSU: NHU_03432(bluB)
RHC: RGUI_2964
LABR: CHH27_12970(bluB)
LVS: LOKVESSMR4R_00075(bluB)
AHT: ANTHELSMS3_03563(bluB)
RBG: BG454_16785(bluB)
SAGU: CDO87_04175(bluB)
NAR: Saro_0330
SPHP: LH20_21890
SPHM: G432_07700
STAX: MC45_16140
SJP: SJA_C2-03090(bluB)
SPMI: K663_18386
SPHB: EP837_03436(bluB)
SINB: SIDU_11840
SPHT: K426_22454
SYA: A6768_22445(bluB)
BLAS: BSY18_2561(bluB)
GOH: B932_2921
GDI: GDI0656
GDJ: Gdia_1350
GXY: GLX_15140
GXL: H845_259
KEU: S101446_02107(bluB)
ASZ: ASN_886
APOM: CPF11_09385(bluB)
ATO: CIW82_07665(bluB)
ROS: CTJ15_04825(bluB)
RRU: Rru_A3536
RRF: F11_18105
RCE: RC1_3717(bluB)
MAG: amb1227
MAGX: XM1_1861(bluB)
MAGN: WV31_17055
ALI: AZOLI_p40236(bluB)
ABS: AZOBR_p140044(bluB)
TMO: TMO_c0843
TXI: TH3_17990
THAC: CSC3H3_17110(bluB)
MAGQ: MGMAQ_2057
NAO: Y958_27810(bluB)
MGM: Mmc1_1683
BMQ: BMQ_0968(bluB)
BMD: BMD_0969(bluB)
BMEG: BG04_3258(bluB)
BACO: OXB_2234
BEO: BEH_16050
BKW: BkAM31D_09100(bluB)
LSP: Bsph_2918
PMW: B2K_36545
ASOC: CB4_00860(bluB)
BTS: Btus_0240
KYR: CVV65_04120(bluB)
TAB: CIG75_03810(bluB)
LFB: C1X05_02805(bluB)
MTU: Rv0306
MTV: RVBD_0306
MTC: MT0319(bluB)
MRA: MRA_0315
MTUR: CFBS_0323
MTD: UDA_0306
MTUC: J113_02240
MTUE: J114_01665
MTUL: TBHG_00302
MTUT: HKBT1_0323
MTUU: HKBT2_0323
MBB: BCG_0346
MBT: JTY_0316
MBX: BCGT_0075
MAF: MAF_03080
MMIC: RN08_0350
MPA: MAP_3792c
MAO: MAP4_3902
MAVI: RC58_19380
MAVU: RE97_19430
MAV: MAV_4857(bluB)
MAVD: NF84_22465
MAVR: LA63_22720
MAVA: LA64_22660
MIT: OCO_47660
MIA: OCU_47580
MIR: OCQ_48670
MID: MIP_07209
MYO: OEM_47810
MMAL: CKJ54_22675(bluB)
MSM: MSMEG_6053(bluB)
MSG: MSMEI_5893(bluB)
MVA: Mvan_0381
MGI: Mflv_0353
MPHL: MPHLCCUG_00478(bluB)
MVQ: MYVA_0352
MUL: MUL_1218
MAB: MAB_3351c
MABB: MASS_3291
MABO: NF82_16780
MCHE: BB28_16885
MSTE: MSTE_03317
MMC: Mmcs_4721
MJL: Mjls_5106
MMI: MMAR_0555
MMM: W7S_23895
MHAD: B586_00820
ASD: AS9A_3944
COA: DR71_711(bluB)
CAQU: CAQU_10690
NFA: NFA_46050
NFR: ERS450000_05323(nox_2)
NSR: NS506_02094(bluB)
RER: RER_23360
REY: O5Y_10925
ROP: ROP_64780
REQ: REQ_32250
RHB: NY08_4457
RFA: A3L23_02553(bluB_1)
RHS: A3Q41_00810(bluB_1)
RRZ: CS378_20560(bluB)
RHU: A3Q40_03153(bluB)
RQI: C1M55_12190(bluB)
GPO: GPOL_c29810(bluB)
GOR: KTR9_2026
GOC: CXX93_12090(bluB)
TPR: Tpau_0087
PPC: HMPREF9154_2365(bluB)
NCA: Noca_3407
NDK: I601_3845(bluB)
PSIM: KR76_17070
FAL: FRAAL4424
SEN: SACE_0750
AMD: AMED_7059
AMN: RAM_36220
AMM: AMES_6951
AMZ: B737_6951
AOI: AORI_4778(bluB)
AMYC: CU254_28560(bluB)
AMI: Amir_0603
APRE: CNX65_02995(bluB)
SESP: BN6_06460
KAL: KALB_710
ALL: CRK56139
SAQ: Sare_4962
ASE: ACPL_4734
ACTN: L083_1238
AFS: AFR_28530
AEY: CDG81_03400(bluB)
AFO: Afer_0271
TER: Tery_4461
TTH: TT_P0018
TTJ: TTHB061
SACI: Sinac_2349
TJE: TJEJU_2902(bluB)
FEK: C1H87_22350(bluB)
TAJ: C1A40_12050(bluB)
CTE: CT0937
CPC: Cpar_1182
CCH: Cag_1013
CLI: Clim_1065
PLT: Plut_1141
PPH: Ppha_1263
PAA: Paes_1270
PROS: CHL67_05125(bluB)
CTS: Ctha_2225
NDE: NIDE2104(bluB) NIDE2667
LFC: LFE_0654
MOX: DAMO_2090
HHI: HAH_1104(nfnB)
HVO: HVO_0641(bluB)
HME: HFX_2169(nfnB)
NAT: NJ7G_1056
FAI: FAD_1404(bluB)
SSO: SSO2560
SOL: Ssol_0371
SSOA: SULA_0365
SSOL: SULB_0367
SSOF: SULC_0365
STO: STK_06010
SAI: Saci_0250
SID: M164_2727
SII: LD85_3070
SIH: SiH_2702
SIR: SiRe_2639
SIC: SiL_2588
MSE: Msed_1226
MCN: Mcup_1028
AHO: Ahos_2079
TUZ: TUZN_1096
NMR: Nmar_1736
NID: NPIRD3C_0186(bluB)
NIN: NADRNF5_0135(bluB)
NCT: NMSP_1655
NEV: NTE_00833
TAA: NMY3_02515(bluB_2)
NCV: NCAV_1669(bluB)
NBV: T478_1447(bluB)
NDV: NDEV_2067(bluB)
 » show all
Taxonomy
Reference
1  [PMID:17301238]
  Authors
Gray MJ, Escalante-Semerena JC
  Title
Single-enzyme conversion of FMNH2 to 5,6-dimethylbenzimidazole, the lower ligand  of B12.
  Journal
Proc. Natl. Acad. Sci. U. S. A. 104 (2007) 2921-6.
  Sequence
[rru:Rru_A3536]
Reference
2  [PMID:17377573]
  Authors
Ealick SE, Begley TP
  Title
Biochemistry: molecular cannibalism.
  Journal
Nature. 446 (2007) 387-8.
Reference
3  [PMID:17377583]
  Authors
Taga ME, Larsen NA, Howard-Jones AR, Walsh CT, Walker GC
  Title
BluB cannibalizes flavin to form the lower ligand of vitamin B12.
  Journal
Nature. 446 (2007) 449-53.
  Sequence
[sme:SMc00166]
Reference
4  [PMID:21344938]
  Authors
Wang XL, Quan JM
  Title
Intermediate-assisted multifunctional catalysis in the conversion of flavin to 5,6-dimethylbenzimidazole by BluB: a density functional theory study.
  Journal
J. Am. Chem. Soc. 133 (2011) 4079-91.
Reference
5  [PMID:23457476]
  Authors
Collins HF, Biedendieck R, Leech HK, Gray M, Escalante-Semerena JC, McLean KJ, Munro AW, Rigby SE, Warren MJ, Lawrence AD
  Title
Bacillus megaterium has both a functional BluB protein required for DMB synthesis and a related flavoprotein that forms a stable radical species.
  Journal
PLoS. One. 8 (2013) e55708.
Other DBs
ExplorEnz - The Enzyme Database: 1.13.11.79
IUBMB Enzyme Nomenclature: 1.13.11.79
ExPASy - ENZYME nomenclature database: 1.13.11.79
BRENDA, the Enzyme Database: 1.13.11.79

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