KEGG   ENZYME: 1.13.12.7Help
Entry
EC 1.13.12.7                Enzyme                                 

Name
Photinus-luciferin 4-monooxygenase (ATP-hydrolysing);
firefly luciferase;
luciferase (firefly luciferin);
Photinus luciferin 4-monooxygenase (adenosine triphosphate-hydrolyzing);
firefly luciferin luciferase;
Photinus pyralis luciferase
Class
Oxidoreductases;
Acting on single donors with incorporation of molecular oxygen (oxygenases);
With incorporation of one atom of oxygen (internal monooxygenases or internal mixed-function oxidases)
BRITE hierarchy
Sysname
Photinus-luciferin:oxygen 4-oxidoreductase (decarboxylating, ATP-hydrolysing)
Reaction(IUBMB)
Photinus luciferin + O2 + ATP = oxidized Photinus luciferin + CO2 + AMP + diphosphate + hnu [RN:R04036]
Reaction(KEGG)
Substrate
Photinus luciferin [CPD:C02740];
O2 [CPD:C00007];
ATP [CPD:C00002]
Product
oxidized Photinus luciferin [CPD:C03797];
CO2 [CPD:C00011];
AMP [CPD:C00020];
diphosphate [CPD:C00013];
hnu [CPD:C00205]
Comment
Photinus (firefly) is a bioluminescent insect. The first step in the reaction is the formation of an acid anhydride between the carboxylic group and AMP, with the release of diphosphate. The enzyme may be assayed by measurement of light emission.
History
EC 1.13.12.7 created 1976, modified 1981, modified 1982
Orthology
K05914  
photinus-luciferin 4-monooxygenase (ATP-hydrolysing)
Reference
1  [PMID:6064360]
  Authors
Hopkins TA, Seliger HH, White EH, Cass MH.
  Title
The chemiluminescence of firefly luciferin. A model for the bioluminescent reaction and identification of the product excited state.
  Journal
J. Am. Chem. Soc. 89 (1967) 7148-50.
Reference
2
  Authors
White, E.H., McCapra, F., Field, G.F. and McElroy, W.D.
  Title
The structure and synthesis of firefly luciferin.
  Journal
J. Am. Chem. Soc. 83 (1961) 2402-2403.
Reference
3  [PMID:5784183]
  Authors
White EH, Rapaport E, Hopkins TA, Seliger HH.
  Title
Chemi- and bioluminescence of firefly luciferin.
  Journal
J. Am. Chem. Soc. 91 (1969) 2178-80.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
61970-00-1

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