KEGG   ENZYME: 1.14.11.44Help
Entry
EC 1.14.11.44               Enzyme                                 

Name
(R)-dichlorprop dioxygenase (2-oxoglutarate);
RdpA;
alpha-ketoglutarate-dependent (R)-dichlorprop dioxygenase;
(R)-phenoxypropionate/alpha-ketoglutarate-dioxygenase;
2-oxoglutarate-dependent (R)-dichlorprop dioxygenase;
(R)-mecoprop dioxygenase;
2-oxoglutarate-dependent (R)-mecoprop dioxygenase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
BRITE hierarchy
Sysname
(R)-2-(4-chloro-2-methylphenoxy)propanoate,2-oxoglutarate:oxygen oxidoreductase (pyruvate-forming)
Reaction(IUBMB)
(1) (R)-2-(4-chloro-2-methylphenoxy)propanoate + 2-oxoglutarate + O2 = 4-chloro-2-methylphenol + pyruvate + succinate + CO2 [RN:R10539];
(2) (R)-(2,4-dichlorophenoxy)propanoate + 2-oxoglutarate + O2 = 2,4-dichlorophenol + pyruvate + succinate + CO2 [RN:R10542]
Reaction(KEGG)
Substrate
(R)-2-(4-chloro-2-methylphenoxy)propanoate [CPD:C18608];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007];
(R)-(2,4-dichlorophenoxy)propanoate [CPD:C20685]
Product
4-chloro-2-methylphenol [CPD:C03359];
pyruvate [CPD:C00022];
succinate [CPD:C00042];
CO2 [CPD:C00011];
2,4-dichlorophenol [CPD:C02625]
Comment
Fe2+-dependent enzyme. The enzymes from the Gram-negative bacteria Delftia acidovorans MC1 and Sphingomonas herbicidovorans MH are involved in the degradation of the (R)-enantiomer of the phenoxyalkanoic acid herbicides mecoprop and dichlorprop [1,2].
History
EC 1.14.11.44 created 2013
Reference
1  [PMID:12501996]
  Authors
Westendorf A, Benndorf D, Muller RH, Babel W
  Title
The two enantiospecific dichlorprop/alpha-ketoglutarate-dioxygenases from Delftia acidovorans MC1--protein and sequence data of RdpA and SdpA.
  Journal
Microbiol. Res. 157 (2002) 317-22.
  Sequence
Reference
2  [PMID:16820480]
  Authors
Muller TA, Fleischmann T, van der Meer JR, Kohler HP
  Title
Purification and characterization of two enantioselective alpha-ketoglutarate-dependent dioxygenases, RdpA and SdpA, from Sphingomonas herbicidovorans MH.
  Journal
Appl. Environ. Microbiol. 72 (2006) 4853-61.
Reference
3  [PMID:16731970]
  Authors
Muller TA, Zavodszky MI, Feig M, Kuhn LA, Hausinger RP
  Title
Structural basis for the enantiospecificities of R- and S-specific phenoxypropionate/alpha-ketoglutarate dioxygenases.
  Journal
Protein. Sci. 15 (2006) 1356-68.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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