KEGG   ENZYME: 1.14.12.18Help
Entry
EC 1.14.12.18               Enzyme                                 

Name
biphenyl 2,3-dioxygenase;
biphenyl dioxygenase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
BRITE hierarchy
Sysname
biphenyl,NADH:oxygen oxidoreductase (2,3-hydroxylating)
Reaction(IUBMB)
biphenyl + NADH + H+ + O2 = (1S,2R)-3-phenylcyclohexa-3,5-diene-1,2-diol + NAD+ [RN:R05263]
Reaction(KEGG)
Substrate
biphenyl [CPD:C06588];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
(1S,2R)-3-phenylcyclohexa-3,5-diene-1,2-diol [CPD:C06589];
NAD+ [CPD:C00003]
Comment
Requires Fe2+. The enzyme from Burkholderia fungorum LB400 (previously Pseudomonas sp.) is part of a multicomponent system composed of an NADH:ferredoxin oxidoreductase (FAD cofactor), a [2Fe-2S] Rieske-type ferredoxin, and a terminal oxygenase that contains a [2Fe-2S] Rieske-type iron-sulfur cluster and a catalytic mononuclear nonheme iron centre. Chlorine-substituted biphenyls can also act as substrates. Similar to the three-component enzyme systems EC 1.14.12.3 (benzene 1,2-dioxygenase) and EC 1.14.12.11 (toluene dioxygenase).
History
EC 1.14.12.18 created 2001
Pathway
Dioxin degradation
Metabolic pathways
Microbial metabolism in diverse environments
Orthology
K08689  
biphenyl 2,3-dioxygenase subunit alpha
K15750  
biphenyl 2,3-dioxygenase subunit beta
Genes
PSD: 
BXE: 
Bxe_C1196(bphA2) Bxe_C1197(bphA1)
BXB: 
DR64_8608(bphA) DR64_8609(bphE)
PNA: 
ACK: 
C380_15925(bphA1) C380_15930(bphA2)
CTES: 
PGV: 
SL003B_4095(bphAa) SL003B_4096(bphAb)
RHA: 
ROP: 
Taxonomy
Reference
1  [PMID:7592331]
  Authors
Haddock JD, Gibson DT.
  Title
Purification and characterization of the oxygenase component of biphenyl 2,3-dioxygenase from Pseudomonas sp. strain LB400.
  Journal
J. Bacteriol. 177 (1995) 5834-9.
  Sequence
Reference
2  [PMID:9451832]
  Authors
Haddock JD, Pelletier DA, Gibson DT.
  Title
Purification and properties of ferredoxinBPH, a component of biphenyl 2,3-dioxygenase of Pseudomonas sp strain LB400.
  Journal
J. Ind. Microbiol. Biotechnol. 19 (1997) 355-9.
Reference
3  [PMID:9683647]
  Authors
Broadus RM, Haddock JD.
  Title
Purification and characterization of the NADH:ferredoxinBPH oxidoreductase component of biphenyl 2,3-dioxygenase from Pseudomonas sp. strain LB400.
  Journal
Arch. Microbiol. 170 (1998) 106-12.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
UM-BBD (Biocatalysis/Biodegradation Database): 
BRENDA, the Enzyme Database: 
CAS: 
103289-55-0

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