| Entry |
|
|---|
| Name |
biphenyl 2,3-dioxygenase;
biphenyl dioxygenase |
|---|
| Class |
Oxidoreductases;
Acting on paired donors, with O2 as oxidant and incorporation or
reduction of oxygen. The oxygen incorporated need not be derived
from O2;
With NADH or NADPH as one donor, and incorporation of two atoms of
oxygen into the other donor
 |
|---|
| Sysname |
biphenyl,NADH:oxygen oxidoreductase (2,3-hydroxylating) |
|---|
| Reaction(IUBMB) |
biphenyl + NADH + H+ + O2 =
(1S,2R)-3-phenylcyclohexa-3,5-diene-1,2-diol + NAD+ [RN:R05263] |
|---|
| Reaction(KEGG) |
R05263;
(other) R05261 R05262 R05264
 |
|---|
| Substrate |
biphenyl [CPD:C06588];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007] |
|---|
| Product |
(1S,2R)-3-phenylcyclohexa-3,5-diene-1,2-diol [CPD:C06589];
NAD+ [CPD:C00003] |
|---|
| Comment |
Requires Fe2+. The enzyme from Burkholderia fungorum LB400
(previously Pseudomonas sp.) is part of a multicomponent system
composed of an NADH:ferredoxin oxidoreductase (FAD cofactor), a
[2Fe-2S] Rieske-type ferredoxin, and a terminal oxygenase that
contains a [2Fe-2S] Rieske-type iron-sulfur cluster and a catalytic
mononuclear nonheme iron centre. Chlorine-substituted biphenyls can
also act as substrates. Similar to the three-component enzyme
systems EC 1.14.12.3 (benzene 1,2-dioxygenase) and EC 1.14.12.11
(toluene dioxygenase). |
|---|
| Pathway |
PATH: ec00621 Biphenyl degradation
PATH: ec01100 Metabolic pathways |
|---|
| Orthology |
KO: K08689 biphenyl 2,3-dioxygenase |
|---|
| Genes |
BXE: Bxe_C1194(bphA3) Bxe_C1196(bphA2) Bxe_C1197(bphA1)
PNA: Pnap_4149 Pnap_4151 Pnap_4152
RHA: RHA1_ro08058(bphAc) RHA1_ro08059(bphAb) RHA1_ro08060(bphAa)
RHA1_ro10133(etbAa1) RHA1_ro10134(etbAb1) RHA1_ro10143(etbAa2)
RHA1_ro10144(etbAb2) RHA1_ro10145(etbAc)
 |
|---|
| Structures |
PDB: 1ULI 1ULJ |
|---|
Reference
Authors
Title
Journal
Organism
|
1 [PMID:7592331]
Haddock JD, Gibson DT.
Purification and characterization of the oxygenase component of
biphenyl 2,3-dioxygenase from Pseudomonas sp. strain LB400.
J. Bacteriol. 177 (1995) 5834-9.
Pseudomonas sp. |
|---|
Reference
Authors
Title
Journal
Organism
|
2 [PMID:9451832]
Haddock JD, Pelletier DA, Gibson DT.
Purification and properties of ferredoxinBPH, a component of
biphenyl 2,3-dioxygenase of Pseudomonas sp strain LB400.
J. Ind. Microbiol. Biotechnol. 19 (1997) 355-9.
Pseudomonas sp. |
|---|
Reference
Authors
Title
Journal
Organism
|
3 [PMID:9683647]
Broadus RM, Haddock JD.
Purification and characterization of the NADH:ferredoxinBPH
oxidoreductase component of biphenyl 2,3-dioxygenase from
Pseudomonas sp. strain LB400.
Arch. Microbiol. 170 (1998) 106-12.
Pseudomonas sp. |
|---|
| Other DBs |
ExplorEnz - The Enzyme Database: 1.14.12.18
IUBMB Enzyme Nomenclature: 1.14.12.18
ExPASy - ENZYME nomenclature database: 1.14.12.18
UM-BBD (Biocatalysis/Biodegradation Database): 1.14.12.18
BRENDA, the Enzyme Database: 1.14.12.18
CAS: 103289-55-0 |
|---|
