KEGG   ENZYME: 1.14.13.142Help
Entry
EC 1.14.13.142              Enzyme                                 

Name
3-ketosteroid 9alpha-monooxygenase;
KshAB;
3-ketosteroid 9alpha-hydroxylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
androsta-1,4-diene-3,17-dione,NADH:oxygen oxidoreductase (9alpha-hydroxylating)
Reaction(IUBMB)
androsta-1,4-diene-3,17-dione + NADH + H+ + O2 = 9alpha-hydroxyandrosta-1,4-diene-3,17-dione + NAD+ + H2O [RN:R09860]
Reaction(KEGG)
Substrate
androsta-1,4-diene-3,17-dione [CPD:C20144];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
9alpha-hydroxyandrosta-1,4-diene-3,17-dione [CPD:C14909];
NAD+ [CPD:C00003];
H2O [CPD:C00001]
Comment
The enzyme is involved in the cholesterol degradation pathway of several bacterial pathogens, such as Mycobacterium tuberculosis. It is a two-component system consisting of a terminal oxygenase (KshA) and a ferredoxin reductase (KshB). The oxygenase contains a Rieske-type iron-sulfur center and non-heme iron. The reductase component is a flavoprotein containing an NAD-binding domain and a plant-type iron-sulfur cluster. The product of the enzyme is unstable, and spontaneously converts to 3-hydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione.
History
EC 1.14.13.142 created 2012
Pathway
Steroid degradation
Metabolic pathways
Microbial metabolism in diverse environments
Orthology
K15982  
3-ketosteroid 9alpha-monooxygenase subunit A
K15983  
3-ketosteroid 9alpha-monooxygenase subunit B
Genes
PSG: 
PRE: 
PPUT: 
PPUN: 
PP4_26740(kshA) PP4_27180(kshB)
PSC: 
PSH: 
PSTU: 
PBM: 
PPUU: 
PKC: 
PKB_4313(ksha1) PKB_4319(kshB) PKB_4338(ksha3)
PFZ: 
PPSY: 
SPL: 
SHL: 
PHA: 
PTN: 
ZAL: 
GPB: 
REU: 
REH: 
H16_B0643(h16_B0643) H16_B0672(hcaE)
CNC: 
CTI: 
CBW: 
BUR: 
BCJ: 
BCEN: 
BCEO: 
BCT: 
BCED: 
DM42_6314(hmp) DM42_6338(kshA)
BPYR: 
BCON: 
BFN: 
PVE: 
CTT: 
CTES: 
AZI: 
THU: 
NAR: 
NPP: 
SPHK: 
SWI: 
SPHM: 
SSAN: 
SYB: 
MTU: 
Rv3526(kshA) Rv3571(kshB)
MTV: 
MTC: 
MRA: 
MTF: 
MTB: 
MTK: 
MTZ: 
MTG: 
MTE: 
MTUR: 
MTL: 
MTO: 
MTD: 
MTN: 
MTJ: 
MTUB: 
MTUC: 
MTUE: 
MTX: 
MTUH: 
MTUL: 
MTUT: 
MTUU: 
MTQ: 
MBO: 
MBB: 
MBT: 
MBM: 
MBK: 
MBX: 
MBZ: 
MAF: 
MCE: 
MCQ: 
MCV: 
MCX: 
MCZ: 
MMIC: 
MPA: 
MAO: 
MAVI: 
MAVU: 
MAV: 
MAVD: 
MAVR: 
MAVA: 
MIT: 
MIR: 
MIA: 
MIE: 
MID: 
MYO: 
MSM: 
MSG: 
MSB: 
MSN: 
MSH: 
MSA: 
MUL: 
MVA: 
MGI: 
MSP: 
MAB: 
MABB: 
MMV: 
MAY: 
MABO: 
MABL: 
MAZ: 
MAK: 
MYS: 
MYC: 
MMC: 
MKM: 
MJL: 
MJD: 
MMI: 
MRH: 
MMM: 
MCB: 
MLI: 
MKN: 
MKS: 
MKI: 
MNE: 
MYV: 
MYE: 
MGO: 
MFT: 
MHAD: 
MPHL: 
ASD: 
NFA: 
NCY: 
NBR: 
NNO: 
RHA: 
RER: 
RER_07540(kshA) RER_13800(kshA) RER_17750(kshB) RER_51130(kshA)
REY: 
REB: 
ROP: 
ROP_44530(kshA) ROP_58730(kshA2) ROP_58950(kshB) ROP_61150(kshB)
ROA: 
REQ: 
RPY: 
RHB: 
RAV: 
GBR: 
GPO: 
GOR: 
GOQ: 
TPR: 
SRT: 
SALU: 
SALL: 
SVT: 
SLD: 
SRW: 
LXY: 
ARL: 
ARY: 
NCA: 
PSIM: 
TCU: 
SVI: 
AMD: 
AMN: 
AMM: 
AMZ: 
AOI: 
AJA: 
AMQ: 
ALU: 
KAL: 
KPHY: 
STP: 
SAQ: 
AMS: 
 » show all
Taxonomy
Reference
1  [PMID:19561185]
  Authors
Petrusma M, Dijkhuizen L, van der Geize R
  Title
Rhodococcus rhodochrous DSM 43269 3-ketosteroid 9alpha-hydroxylase, a two-component iron-sulfur-containing monooxygenase with subtle steroid substrate  specificity.
  Journal
Appl. Environ. Microbiol. 75 (2009) 5300-7.
Reference
2  [PMID:19234303]
  Authors
Capyk JK, D'Angelo I, Strynadka NC, Eltis LD
  Title
Characterization of 3-ketosteroid 9{alpha}-hydroxylase, a Rieske oxygenase in the cholesterol degradation pathway of Mycobacterium tuberculosis.
  Journal
J. Biol. Chem. 284 (2009) 9937-46.
  Sequence
Reference
3  [PMID:21987574]
  Authors
Capyk JK, Casabon I, Gruninger R, Strynadka NC, Eltis LD
  Title
Activity of 3-ketosteroid 9alpha-hydroxylase (KshAB) indicates cholesterol side chain and ring degradation occur simultaneously in Mycobacterium tuberculosis.
  Journal
J. Biol. Chem. 286 (2011) 40717-24.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
UM-BBD (Biocatalysis/Biodegradation Database): 
BRENDA, the Enzyme Database: 

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