KEGG   ENZYME: 1.14.13.81Help
Entry
EC 1.14.13.81               Enzyme                                 

Name
magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase;
Mg-protoporphyrin IX monomethyl ester (oxidative) cyclase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
magnesium-protoporphyrin-IX 13-monomethyl ester,NADPH:oxygen oxidoreductase (hydroxylating)
Reaction(IUBMB)
magnesium-protoporphyrin IX 13-monomethyl ester + 3 NADPH + 3 H+ + 3 O2 = divinylprotochlorophyllide + 3 NADP+ + 5 H2O (overall reaction) [RN:R10068];
(1a) magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + H+ + O2 = 131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + H2O [RN:R06265];
(1b) 131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + H+ + O2 = 131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + 2 H2O [RN:R06266];
(1c) 131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + H+ + O2 = divinylprotochlorophyllide + NADP+ + 2 H2O [RN:R06267]
Reaction(KEGG)
Substrate
magnesium-protoporphyrin IX 13-monomethyl ester [CPD:C04536];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007];
131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester;
131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester
Product
divinylprotochlorophyllide [CPD:C11831];
NADP+ [CPD:C00006];
H2O [CPD:C00001];
131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester;
131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester
Comment
Requires Fe(II) for activity. The cyclase activity in Chlamydomonas reinhardtii is associated exclusively with the membranes, whereas that from cucumber cotyledons requires both membrane and soluble fractions for activity.
History
EC 1.14.13.81 created 2003
Pathway
Porphyrin and chlorophyll metabolism
Metabolic pathways
Biosynthesis of secondary metabolites
Orthology
K04035  
magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase
Genes
ATH: 
AT3G56940(CRD1)
ALY: 
CRB: 
EUS: 
BRP: 
BNA: 
THJ: 
CIT: 
CIC: 
TCC: 
EGR: 
GMX: 
PVU: 
MTR: 
CAM: 
ADU: 
AIP: 
FVE: 
PPER: 
PMUM: 
PXB: 
CSV: 
CMO: 
RCU: 
JCU: 
POP: 
POPTR_0006s02810g(POPTRDRAFT_761926) POPTR_0016s02570g(POPTRDRAFT_734671)
VVI: 
SLY: 
SOT: 
102600334(at103)
BVG: 
OSA: 
4326901(P0003H10.2)
DOSA: 
Os01t0279100-01(Os01g0279100)
OBR: 
BDI: 
SBI: 
SORBI_03g011270(SORBIDRAFT_03g011270)
ZMA: 
103634652(GRMZM2G081462)
SITA: 
PDA: 
EGU: 
MUS: 
ATR: 
SMO: 
PPP: 
CRE: 
VCN: 
OLU: 
OTA: 
BPG: 
MIS: 
MPP: 
CSL: 
CVR: 
NOC: 
BUR: 
RGE: 
RGE_33550(acsF)
AZO: 
DVL: 
BRA: 
BRADO1649(acsF)
BBT: 
BBta_6407(acsF)
BRS: 
S23_19480(acsF)
AOL: 
RPA: 
RPB: 
RPC: 
RPD: 
RPE: 
RPT: 
RPX: 
MEX: 
MEA: 
MDI: 
METDI5863(acsF)
MCH: 
MRD: 
MET: 
MPO: 
MSL: 
BSB: 
RSP: 
RSH: 
RSQ: 
RSK: 
JAN: 
Jann_0151(acsF)
RDE: 
RD1_0129(acsF)
RLI: 
DSH: 
Dshi_3544(acsF)
AMV: 
RCE: 
RC1_2105(acsF)
FRA: 
SYN: 
SYZ: 
SYY: 
SYNGTS_0005(sll1214) SYNGTS_1645(chlAII)
SYT: 
SYNGTI_0005(sll1214) SYNGTI_1645(chlAII)
SYS: 
SYNPCCN_0005(sll1214) SYNPCCN_1644(chlAII)
SYQ: 
SYNPCCP_0005(sll1214) SYNPCCP_1644(chlAII)
SYW: 
SYNW1198(PNIL34)
SYC: 
syc2188_d(ycf59)
SYF: 
SYD: 
SYE: 
SYG: 
sync_1304(acsF)
SYR: 
SYX: 
SYP: 
CYA: 
CYA_2703(acsF)
CYB: 
CYB_1254(acsF)
SYNE: 
SYNP: 
TEL: 
CGC: 
DSL: 
CMP: 
LEP: 
PSEU: 
PMA: 
Pro_0992(acsF)
PMM: 
PMM0844(PNIL34)
PMT: 
PMN: 
PMI: 
PMB: 
A9601_10251(pniL34)
PMC: 
P9515_09201(pnilL34)
PMF: 
P9303_29201(pniL34)
PMG: 
P9301_10241(pniL34)
PMH: 
P9215_10571(pniL34)
PMJ: 
P9211_09191(pniIL34)
PME: 
NATL1_08941(pnil34)
AMR: 
AM1_0465(acsF) AM1_2295(acsF)
MAR: 
CAN: 
CSN: 
HAO: 
CYU: 
GLP: 
CYT: 
CYP: 
CYC: 
CYN: 
CYH: 
CYJ: 
TER: 
MIC: 
ARP: 
GEI: 
OAC: 
ONI: 
CEP: 
GVI: 
gvip493(ycf59)
ANA: 
NPU: 
NOS: 
NOP: 
AVA: 
ANB: 
ACY: 
NAZ: 
CSG: 
CALO: 
CALT: 
RIV: 
CTHE: 
PLP: 
SCS: 
RRS: 
RCA: 
CAU: 
CAG: 
CHL: 
CTM: 
CCH: 
CPH: 
PVI: 
PLT: 
MBU: 
SMR: 
 » show all
Taxonomy
Reference
1  [PMID:12226378]
  Authors
Bollivar DW, Beale SI.
  Title
The Chlorophyll Biosynthetic Enzyme Mg-Protoporphyrin IX Monomethyl Ester (Oxidative) Cyclase (Characterization and Partial Purification from Chlamydomonas reinhardtii and Synechocystis sp. PCC 6803).
  Journal
Plant. Physiol. 112 (1996) 105-114.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
92353-62-3

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