KEGG   ENZYME: 1.14.13.84Help
Entry
EC 1.14.13.84               Enzyme                                 

Name
4-hydroxyacetophenone monooxygenase;
HAPMO
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
(4-hydroxyphenyl)ethan-1-one,NADPH:oxygen oxidoreductase (ester-forming)
Reaction(IUBMB)
(4-hydroxyphenyl)ethan-1-one + NADPH + H+ + O2 = 4-hydroxyphenyl acetate + NADP+ + H2O [RN:R06892]
Reaction(KEGG)
Substrate
(4-hydroxyphenyl)ethan-1-one [CPD:C10700];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
4-hydroxyphenyl acetate [CPD:C13636];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
Contains FAD. The enzyme from Pseudomonas fluorescens ACB catalyses the conversion of a wide range of acetophenone derivatives. Highest activity occurs with compounds bearing an electron-donating substituent at the para position of the aromatic ring [1]. In the absence of substrate, the enzyme can act as an NAD(P)H oxidase (EC 1.6.3.1).
History
EC 1.14.13.84 created 2004
Pathway
Bisphenol degradation
Metabolic pathways
Microbial metabolism in diverse environments
Orthology
K14520  
4-hydroxyacetophenone monooxygenase
Genes
PALK: 
GAG: 
GPS: 
BGE: 
BXE: 
BXB: 
PVE: 
BHZ: 
ACR54_00614(hapE_3)
AXY: 
RHI: 
REL: 
REI: 
RTR: 
RGA: 
BJA: 
BJU: 
BJP: 
BRS: 
BRC: 
PZU: 
NAR: 
NPN: 
SPHP: 
STER: 
SWI: 
SPHM: 
SJP: 
SCH: 
AEP: 
TMO: 
MCX: 
MPA: 
MAO: 
MAVI: 
MAVU: 
MAV: 
MAVD: 
MAVR: 
MAVA: 
MIT: 
MIR: 
MIA: 
MIE: 
MID: 
MYO: 
MSA: 
MVA: 
MGI: 
MSP: 
MMC: 
MKM: 
MJL: 
MJD: 
MMI: 
MRH: 
MMM: 
MCB: 
MLI: 
MKN: 
MKS: 
MKI: 
MNE: 
MYV: 
MYE: 
MFT: 
MPHL: 
NNO: 
RHA: 
REY: 
ROP: 
REQ: 
RHB: 
GOR: 
MIO: 
FRI: 
PDX: 
AYM: 
ABAI: 
 » show all
Taxonomy
Reference
1  [PMID:11322873]
  Authors
Kamerbeek NM, Moonen MJ, Van Der Ven JG, Van Berkel WJ, Fraaije MW, Janssen DB.
  Title
4-Hydroxyacetophenone monooxygenase from Pseudomonas fluorescens ACB. A novel flavoprotein catalyzing Baeyer-Villiger oxidation of aromatic compounds.
  Journal
Eur. J. Biochem. 268 (2001) 2547-57.
  Sequence
Reference
2  [PMID:12514023]
  Authors
Kamerbeek NM, Olsthoorn AJ, Fraaije MW, Janssen DB.
  Title
Substrate specificity and enantioselectivity of 4-hydroxyacetophenone monooxygenase.
  Journal
Appl. Environ. Microbiol. 69 (2003) 419-26.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
156621-13-5

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