| Entry |
|
|---|
| Name |
phenylacetone monooxygenase;
PAMO |
|---|
| Class |
Oxidoreductases;
Acting on paired donors, with O2 as oxidant and incorporation or
reduction of oxygen. The oxygen incorporated need not be derived
from O2;
With NADH or NADPH as one donor, and incorporation of one atom of
oxygen into the other donor
 |
|---|
| Sysname |
phenylacetone,NADPH:oxygen oxidoreductase |
|---|
| Reaction(IUBMB) |
phenylacetone + NADPH + H+ + O2 = benzyl acetate + NADP+ + H2O
[RN:R07201] |
|---|
| Reaction(KEGG) |
R07201
 |
|---|
| Substrate |
phenylacetone [CPD:C15512];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007] |
|---|
| Product |
benzyl acetate [CPD:C15513];
NADP+ [CPD:C00006];
H2O [CPD:C00001] |
|---|
| Comment |
A flavoprotein (FAD). NADH cannot replace NADPH as coenzyme. In
addition to phenylacetone, which is the best substrate found to
date, this Baeyer-Villiger monooxygenase can oxidize other aromatic
ketones [1-(4-hydroxyphenyl)propan-2-one,
1-(4-hydroxyphenyl)propan-2-one and 3-phenylbutan-2-one], some
alipatic ketones (e.g. dodecan-2-one) and sulfides (e.g.
1-methyl-4-(methylsulfanyl)benzene). |
|---|
Reference
Authors
Title
Journal
Organism
|
1 [PMID:15328411]
Malito E, Alfieri A, Fraaije MW, Mattevi A.
Crystal structure of a Baeyer-Villiger monooxygenase.
Proc. Natl. Acad. Sci. U. S. A. 101 (2004) 13157-62.
Thermobifida fusca [GN:tfu] |
|---|
Reference
Authors
Title
Journal
Organism
|
2 [PMID:15599520]
Fraaije MW, Wu J, Heuts DP, van Hellemond EW, Spelberg JH, Janssen
DB.
Discovery of a thermostable Baeyer-Villiger monooxygenase by genome
mining.
Appl. Microbiol. Biotechnol. 66 (2005) 393-400.
Thermobifida fusca [GN:tfu] |
|---|
| Other DBs |
ExplorEnz - The Enzyme Database: 1.14.13.92
IUBMB Enzyme Nomenclature: 1.14.13.92
ExPASy - ENZYME nomenclature database: 1.14.13.92
BRENDA, the Enzyme Database: 1.14.13.92
CAS: 1005768-90-0 |
|---|
