| Entry |
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| Name |
camphor 5-monooxygenase;
camphor 5-exo-methylene hydroxylase;
2-bornanone 5-exo-hydroxylase;
bornanone 5-exo-hydroxylase;
camphor 5-exo-hydroxylase;
camphor 5-exohydroxylase;
camphor hydroxylase;
d-camphor monooxygenase;
methylene hydroxylase;
methylene monooxygenase;
D-camphor-exo-hydroxylase;
camphor methylene hydroxylase
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| Class |
Oxidoreductases;
Acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2;
With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor
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| Sysname |
(+)-camphor,reduced putidaredoxin:oxygen oxidoreductase (5-hydroxylating)
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| Reaction(IUBMB) |
(+)-camphor + reduced putidaredoxin + O2 = (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O [RN: R03818]
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| Reaction(KEGG) |
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| Substrate |
(+)-camphor [CPD: C00808];
reduced putidaredoxin;
O2 [CPD: C00007]
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| Product |
(+)-exo-5-hydroxycamphor [CPD: C03448];
oxidized putidaredoxin [CPD: C03302];
H2O [CPD: C00001]
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| Comment |
A heme-thiolate protein (P-450). Also acts on (-)-camphor and 1,2-campholide, forming 5-exo-hydroxy-1,2-campholide.
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| Reference |
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| Authors |
Hedegaard J, Gunsalus IC. |
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| Title |
Mixed function oxidation. IV. An induced methylene hydroxylase in camphor oxidation. |
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| Journal |
J. Biol. Chem. 240 (1965) 4038-43. |
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| Organism |
Pseudomonas putida |
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| Reference |
|
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| Authors |
Tyson CA, Lipscomb JD, Gunsalus IC. |
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| Title |
The role of putidaredoxin and P450 cam in methylene hydroxylation. |
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| Journal |
J. Biol. Chem. 247 (1972) 5777-84. |
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| Organism |
Pseudomonas putida |
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| Other DBs |
ExplorEnz - The Enzyme Database: IUBMB Enzyme Nomenclature: ExPASy - ENZYME nomenclature database: UM-BBD (Biocatalysis/Biodegradation Database): BRENDA, the Enzyme Database: CAS: 9030-82-4 |
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