KEGG   ENZYME: 1.14.19.2Help
Entry
EC 1.14.19.2                Enzyme                                 

Name
stearoyl-[acyl-carrier-protein] 9-desaturase;
stearyl acyl carrier protein desaturase;
stearyl-ACP desaturase;
acyl-[acyl-carrier-protein] desaturase;
acyl-[acyl-carrier protein],hydrogen-donor:oxygen oxidoreductase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
BRITE hierarchy
Sysname
stearoyl-[acyl-carrier protein],reduced ferredoxin:oxygen oxidoreductase (9,10 cis-dehydrogenating)
Reaction(IUBMB)
stearoyl-[acyl-carrier protein] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ = oleoyl-[acyl-carrier protein] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O [RN:R03370]
Reaction(KEGG)
R03370;
(other) R08161
Show
Substrate
stearoyl-[acyl-carrier protein] [CPD:C04088];
reduced ferredoxin [iron-sulfur] cluster [CPD:C00138];
O2 [CPD:C00007];
H+ [CPD:C00080]
Product
oleoyl-[acyl-carrier protein] [CPD:C01203];
oxidized ferredoxin [iron-sulfur] cluster [CPD:C00139];
H2O [CPD:C00001]
Comment
The enzyme is found in the lumen of plastids, where de novo biosynthesis of fatty acids occurs, and acts on freshly synthesized saturated fatty acids that are still linked to acyl-carrier protein. The enzyme determines the position of the double bond by its distance from the carboxylic acid end of the fatty acid. It also acts on palmitoyl-[acyl-carrier-protein] [4,5].
History
EC 1.14.19.2 created 1972 as EC 1.14.99.6, modified 2000, transferred 2000 to EC 1.14.19.2, modified 2015
Pathway
ec00061  Fatty acid biosynthesis
ec01040  Biosynthesis of unsaturated fatty acids
Orthology
K03921  acyl-[acyl-carrier-protein] desaturase
K03922  acyl-[acyl-carrier-protein] desaturase
Genes
RSS: 109441136 109441685 109442033 109442273 109442758
ATH: AT1G43800(FTM1) AT2G43710(SSI2) AT3G02610 AT3G02620 AT3G02630 AT5G16230 AT5G16240
ALY: ARALYDRAFT_477504 ARALYDRAFT_488473 ARALYDRAFT_670995 ARALYDRAFT_891368 ARALYDRAFT_903770 ARALYDRAFT_909604
CRB: 17881420 17884225 17887583 17892070 17892815 17897283
CSAT: 104703073 104705911 104705912 104735648 104735649 104742149 104744342 104744343 104757931 104763879 104763880 104769885 104769886 104777112 104777214 104782900 104784970 104793309(SAD3)
EUS: EUTSA_v10001477mg EUTSA_v10011783mg EUTSA_v10012283mg EUTSA_v10013713mg EUTSA_v10013733mg EUTSA_v10020875mg
BRP: 103843412 103846343 103846344 103858091 103858976 103866745 103870910
BNA: 106360893 106366808 106366809 106372016 106372017 106372205 106379047 106387283 106388339 106396242 106438946 106447152 111197705
BOE: 106294068 106313000 106319191 106335862 106336467 106342438
THJ: 104802328 104802432 104802433 104804087 104806847 104827165
TCC: 18592535(SAD7) 18598496(SAD2) 18601170(SAD5) 18602344(SAD1) 18602345(SAD4) 18602347(SAD3)
GMX: 100037478(SACPD-C) 100217331(SAD2) 100816146 547808(ACPD)
LJA: Lj0g3v0353059.1(Lj0g3v0353059.1) Lj1g3v0264200.1(Lj1g3v0264200.1) Lj4g3v0510250.1(Lj4g3v0510250.1)
RCU: 107262125 8263458(SAD2) 8271760 8277360(SAD3)
DOSA: Os01t0880800-01(Os01g0880800) Os01t0919900-01(Os01g0919900) Os02t0504800-01(Os02g0504800) Os03t0423300-01(Os03g0423300) Os03t0741000-00(Os03g0741000) Os04t0379900-01(Os04g0379900) Os06t0503800-00(Os06g0503800) Os08t0199400-01(Os08g0199400) Os08t0200100-01(Os08g0200100)
ATS: 109737747(LOC109737747) 109742804(LOC109742804) 109743731(LOC109743731) 109747362(LOC109747362) 109748642(LOC109748642) 109748975(LOC109748975) 109749248(LOC109749248) 109759486(LOC109759486) 109763251(LOC109763251) 109763252(LOC109763252) 109766563(LOC109766563) 109779328(LOC109779328) 109782689(LOC109782689)
TPS: THAPSDRAFT_bd1192(DES7)
SCL: sce2527
BCZ: BCE33L2881(staD)
BTI: BTG_03995
BWW: bwei_3845(staD)
BMYC: DJ92_5455
BMYO: BG05_2756
ESI: Exig_2392
EAN: Eab7_2235
PMW: B2K_01300
ASOC: CB4_01449(desA1)
BTS: Btus_3008
SAY: TPY_2839
MTU: Rv0824c(desA1) Rv1094(desA2)
MRA: MRA_0834(desA1) MRA_1105(desA2)
MTUR: CFBS_0865(desA1) CFBS_1161(desA2)
MTO: MTCTRI2_0841(desA1) MTCTRI2_1124(desA2)
MTD: UDA_0824c(desA1) UDA_1094(desA2)
MTN: ERDMAN_0907(desA1) ERDMAN_1226(desA2)
MTUT: HKBT1_0866(desA1) HKBT1_1159(desA2)
MTUU: HKBT2_0866(desA1) HKBT2_1165(desA2)
MTQ: HKBS1_0865(desA1) HKBS1_1161(desA2)
MBO: BQ2027_MB0847C(desA1) BQ2027_MB1124(desA2)
MBB: BCG_0877c(desA1) BCG_1154(desA2)
MBT: JTY_0847(desA1) JTY_1127(desA2)
MBM: BCGMEX_0848c(desA1) BCGMEX_1126(desA2)
MAF: MAF_08340(desA1) MAF_11090(desA2)
MCE: MCAN_08271(desA1) MCAN_11031(desA2)
MCQ: BN44_10898(desA) BN44_11218(desA)
MCV: BN43_20267(desA) BN43_30150(desA)
MCX: BN42_20586(desA) BN42_20941(desA)
MCZ: BN45_20093(desA) BN45_30136(desA)
MLE: ML1952(desA2) ML2185(desA1)
MLB: MLBr01952(desA2) MLBr02185(desA1)
MPA: MAP_0658c(desA1) MAP_2698c(desA2)
MUL: MUL_0187(desA2) MUL_0445(desA1) MUL_3646(desA1_1)
MMI: MMAR_3704(desA1_1) MMAR_4374(desA2) MMAR_4856(desA1)
MMAE: MMARE11_36110(desA1_1) MMARE11_41940(desA2) MMARE11_46640(desA1)
MLI: MULP_03579(desA2) MULP_03954(desA1_1) MULP_04589(desA2_1) MULP_05089(desA1)
MSG: MSMEI_5110(desA2) MSMEI_5619(des)
MPHL: MPHLCCUG_00726(desA1_1) MPHLCCUG_04245(desA2) MPHLCCUG_05145(desA1_2)
MJD: JDM601_0873(desA1_1) JDM601_1040(desA2) JDM601_3526(desA1)
NFA: NFA_6080
RER: RER_47900(desA1)
REY: O5Y_22685
ROP: ROP_49510(desA1)
REQ: REQ_08730
RFA: A3L23_00640(desA1_1) A3L23_00758(desA1_2) A3L23_01498(desA1_3) A3L23_01832(desA1_4) A3L23_03015(desA1_5)
RHS: A3Q41_00308(desA1_1) A3Q41_01533(desA1_2) A3Q41_01860(desA1_3) A3Q41_02594(desA1_4) A3Q41_02715(desA1_5)
RHU: A3Q40_02386(desA1_1) A3Q40_02652(desA1_2) A3Q40_02779(desA1_3)
SCO: SCO6717(SC4C6.27c)
SMA: SAVERM_1691(desA)
SGR: SGR_1008
SGB: WQO_30535
SFA: Sfla_0689
SHY: SHJG_7615
SVE: SVEN_6398
SDV: BN159_1702(desA1)
SALB: XNR_0316
SALS: SLNWT_0409
STRP: F750_6176
SFI: SFUL_6491
SALU: DC74_3164
SALL: SAZ_16890
SLV: SLIV_04925(desA1)
SGU: SGLAU_28080(desA1)
STRE: GZL_05766
STRM: M444_27940
SAMB: SAM23877_6369(desA)
SPRI: SPRI_1049
SRW: TUE45_07262(desA1)
SLE: sle_11110(sle_11110)
SRN: A4G23_05001(desA1)
SNR: SNOUR_27115(desA1)
SMAL: SMALA_2648
SLAU: SLA_6346
SALJ: SMD11_4487
SLX: SLAV_07635(desA1)
KSK: KSE_54350(desA)
LMOI: VV02_01125
NDK: I601_2941(desA1)
KFL: Kfla_7030
TCU: Tcur_3578
SRO: Sros_1903
FAL: FRAAL0670
NML: Namu_0992
MMAR: MODMU_5493
AMD: AMED_4117
AMN: RAM_20980
AMM: AMES_4069
AMZ: B737_4069
AOI: AORI_1632
SAQ: Sare_1811
MIL: ML5_2412
CAI: Caci_2173
AYM: YM304_36700(desA)
HAU: Haur_0733
DGE: Dgeo_0121
DGO: DGo_CA2715(staD)
TRA: Trad_2440
PLH: VT85_13645(desA1_1) VT85_24455(desA1_2)
GES: VT84_22280(desA1)
PBOR: BSF38_01549(desA1)
ABAC: LuPra_05096(desA1)
GAU: GAU_3746
GBA: J421_1125
PHE: Phep_1163
SMIZ: 4412673_02968(desA1)
MUC: MuYL_4091
MGOT: MgSA37_04262(desA1)
DFE: Dfer_5690
SLI: Slin_4583
LBY: Lbys_3166
FAE: FAES_2147
HSW: Hsw_2548
GFO: GFO_3404
GFL: GRFL_1514
FJO: Fjoh_0968
FJG: BB050_01640(desA1)
FIN: KQS_13090
ZPR: ZPR_0309
MARM: YQ22_07090
CBAL: M667_03965
CBAT: M666_03910
ZGA: ZOBELLIA_1485(ACPD)
NDO: DDD_1638
EAO: BD94_2620
ELB: VO54_00092(desA1)
MYR: MYRA21_1005(desA)
MPW: MPR_3539
CHZ: CHSO_2306
WIN: WPG_1455
TMAR: MARIT_0197
AALG: AREALGSMS7_01036(desA1)
FBA: FIC_01332
IAL: IALB_0537
 » show all
Taxonomy
Reference
1  [PMID:4831331]
  Authors
Jaworski JG, Stumpf PK.
  Title
Fat metabolism in higher plants. Properties of a soluble stearyl-acyl carrier protein desaturase from maturing Carthamus tinctorius.
  Journal
Arch. Biochem. Biophys. 162 (1974) 158-65.
Reference
2  [PMID:4300868]
  Authors
Nagai J, Bloch K.
  Title
Enzymatic desaturation of stearyl acyl carrier protein.
  Journal
J. Biol. Chem. 243 (1968) 4626-33.
Reference
3  [PMID:2006187]
  Authors
Shanklin J, Somerville C
  Title
Stearoyl-acyl-carrier-protein desaturase from higher plants is structurally unrelated to the animal and fungal homologs.
  Journal
Proc. Natl. Acad. Sci. U. S. A. 88 (1991) 2510-4.
  Sequence
[rcu:8271760]
Reference
4  [PMID:9144157]
  Authors
Cahoon EB, Lindqvist Y, Schneider G, Shanklin J
  Title
Redesign of soluble fatty acid desaturases from plants for altered substrate specificity and double bond position.
  Journal
Proc. Natl. Acad. Sci. U. S. A. 94 (1997) 4872-7.
Reference
5  [PMID:19716420]
  Authors
Cao Y, Xian M, Yang J, Xu X, Liu W, Li L
  Title
Heterologous expression of stearoyl-acyl carrier protein desaturase (S-ACP-DES) from Arabidopsis thaliana in Escherichia coli.
  Journal
Protein. Expr. Purif. 69 (2010) 209-14.
  Sequence
[ath:AT2G43710]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.19.2
IUBMB Enzyme Nomenclature: 1.14.19.2
ExPASy - ENZYME nomenclature database: 1.14.19.2
BRENDA, the Enzyme Database: 1.14.19.2
CAS: 37256-86-3

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