KEGG   ENZYME: 1.14.20.1Help
Entry
EC 1.14.20.1                Enzyme                                 

Name
deacetoxycephalosporin-C synthase;
DAOCS;
penicillin N expandase;
DAOC synthase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and the other dehydrogenated
BRITE hierarchy
Sysname
penicillin-N,2-oxoglutarate:oxygen oxidoreductase (ring-expanding)
Reaction(IUBMB)
penicillin N + 2-oxoglutarate + O2 = deacetoxycephalosporin C + succinate + CO2 + H2O [RN:R05301]
Reaction(KEGG)
Substrate
penicillin N [CPD:C06564];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007]
Product
deacetoxycephalosporin C [CPD:C06565];
succinate [CPD:C00042];
CO2 [CPD:C00011];
H2O [CPD:C00001]
Comment
Forms part of the penicillin biosynthesis pathway (for pathway, click here).
History
EC 1.14.20.1 created 2002
Pathway
Penicillin and cephalosporin biosynthesis
Metabolic pathways
Biosynthesis of antibiotics
Orthology
K12744  
deacetoxycephalosporin-C synthase
Genes
SCT: 
SCAT_5687(cefE)
SCY: 
Taxonomy
Reference
1  [PMID:1354366]
  Authors
Cantwell C, Beckmann R, Whiteman P, Queener SW, Abraham EP.
  Title
Isolation of deacetoxycephalosporin C from fermentation broths of Penicillium chrysogenum transformants: construction of a new fungal biosynthetic pathway.
  Journal
Proc. R. Soc. Lond. B. Biol. Sci. 248 (1992) 283-9.
Reference
2  [PMID:11352583]
  Authors
Lee HJ, Lloyd MD, Harlos K, Clifton IJ, Baldwin JE, Schofield CJ.
  Title
Kinetic and crystallographic studies on deacetoxycephalosporin C synthase (DAOCS).
  Journal
J. Mol. Biol. 308 (2001) 937-48.
Reference
3
  Authors
Yeh, W.K., Ghag, S.K. and Queener, S.W.
  Title
Enzymes for epimerization of isopenicillin N, ring expansion of penicillin N, and 3'-hydroxylation of deacetoxycephalosporin C. Function, evolution, refolding, and enzyme engineering.
  Journal
Ann. N.Y. Acad. Sci. 672 (1992) 396-408.
Reference
4  [PMID:9723623]
  Authors
Valegard K, van Scheltinga AC, Lloyd MD, Hara T, Ramaswamy S, Perrakis A, Thompson A, Lee HJ, Baldwin JE, Schofield CJ, Hajdu J, Andersson I.
  Title
Structure of a cephalosporin synthase.
  Journal
Nature. 394 (1998) 805-9.
Reference
5  [PMID:2656705]
  Authors
Dotzlaf JE, Yeh WK.
  Title
Purification and properties of deacetoxycephalosporin C synthase from recombinant Escherichia coli and its comparison with the native enzyme purified from Streptomyces clavuligerus.
  Journal
J. Biol. Chem. 264 (1989) 10219-27.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
85746-10-7

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