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Entry
EC 1.3.7.7                  Enzyme                                 

Name
ferredoxin:protochlorophyllide reductase (ATP-dependent);
light-independent protochlorophyllide reductase
Class
Oxidoreductases;
Acting on the CH-CH group of donors;
With an iron-sulfur protein as acceptor
BRITE hierarchy
Sysname
ATP-dependent ferredoxin:protochlorophyllide-a 7,8-oxidoreductase
Reaction(IUBMB)
chlorophyllide a + oxidized ferredoxin + 2 ADP + 2 phosphate = protochlorophyllide a + reduced ferredoxin + 2 ATP + 2 H2O [RN:R06282]
Reaction(KEGG)
Substrate
chlorophyllide a [CPD:C02139];
oxidized ferredoxin [CPD:C00139];
ADP [CPD:C00008];
phosphate [CPD:C00009]
Product
protochlorophyllide a [CPD:C02880];
reduced ferredoxin [CPD:C00138];
ATP [CPD:C00002];
H2O [CPD:C00001]
Comment
Occurs in photosynthetic bacteria, cyanobacteria, green algae and gymnosperms. The enzyme catalyses trans-reduction of the D-ring of protochlorophyllide; the product has the (7S,8S)-configuration. Unlike EC 1.3.1.33 (protochlorophyllide reductase), light is not required. The enzyme contains a [4Fe-4S] cluster, and structurally resembles the Fe protein/MoFe protein complex of nitrogenase (EC 1.18.6.1), which catalyses an ATP-driven reduction.
History
EC 1.3.7.7 created 2011, modified 2013
Pathway
Porphyrin and chlorophyll metabolism
Metabolic pathways
Biosynthesis of secondary metabolites
Orthology
K04037  
light-independent protochlorophyllide reductase subunit L
K04038  
light-independent protochlorophyllide reductase subunit N
K04039  
light-independent protochlorophyllide reductase subunit B
Genes
SMO: 
PPP: 
CRE: 
ChreCp009(chlL) ChreCp056(chlN)
VCN: 
CSL: 
CVR: 
ALV: 
TVI: 
TMB: 
HHA: 
HHC: 
RGE: 
RGE_33460(bchN) RGE_33470(bchB) RGE_33490(bchL)
BRA: 
BRADO1639(bchN) BRADO1640(bchB) BRADO1642(chlL)
BBT: 
BBta_6414(chlL) BBta_6416(bchB) BBta_6417(bchN)
BRS: 
S23_19380(bchN) S23_19390(bchB) S23_19410(bchL)
AOL: 
RPA: 
RPA1542(bchN) RPA1543(bchB) RPA1545(chlL)
RPB: 
RPC: 
RPD: 
RPE: 
RPT: 
RPX: 
MEX: 
MEA: 
Mex_1p5270(bchL) Mex_1p5272(bchB) Mex_1p5273(bchN)
MDI: 
METDI5870(bchL) METDI5872(bchB) METDI5873(bchN)
MCH: 
MRD: 
MET: 
MPO: 
MOR: 
MOC_2249(bchL) MOC_2251(bchB) MOC_2252(bchN)
MSL: 
RVA: 
BSB: 
RSP: 
RSP_0285(bchN) RSP_0286(bchB) RSP_0288(bchL)
RSH: 
RSQ: 
RSK: 
RCP: 
JAN: 
RDE: 
RD1_0136(chlL) RD1_0138(bchB) RD1_0139(bchN)
RLI: 
DSH: 
Dshi_3534(bchN) Dshi_3535(bchB) Dshi_3537(chlL)
RED: 
PTP: 
AMV: 
ACMV_18960(bchN) ACMV_18970(bchB) ACMV_18990(bchL)
RRU: 
RRF: 
RCE: 
RC1_2112(chlL) RC1_2114(bchB) RC1_2115(bchN)
RPM: 
HMO: 
HM1_0685(bchB) HM1_0686(bchN) HM1_0687(chlL)
RXY: 
RRD: 
CTM: 
SYN: 
slr0749(chlL) slr0750(chlN) slr0772(chlB)
SYZ: 
MYO_121700(chlB) MYO_130750(chlL) MYO_130760(chlN)
SYY: 
SYT: 
SYS: 
SYQ: 
SYJ: 
SYW: 
SYNW1723(chlN) SYNW1724(chlB) SYNW1725(chlL)
SYC: 
syc0136_c(chlN) syc0137_c(chlL) syc2256_d(chlB)
SYF: 
SYD: 
SYE: 
SYG: 
sync_1973(chlN) sync_1974(chlB) sync_1975(chlL)
SYR: 
SYX: 
SYP: 
CYA: 
CYA_0895(chlB) CYA_2382(chlL) CYA_2385(chlN)
CYB: 
CYB_1013(chlN) CYB_1016(chlL) CYB_2193(chlB)
SYNE: 
SYNP: 
SYNK: 
SYNR: 
SYND: 
TEL: 
tll2345(chlN) tll2347(chlL) tll2392(chlB)
THN: 
MAR: 
MAE_16230(chlL) MAE_16250(chlN) MAE_53860(chlB)
CYT: 
cce_1954(chlB) cce_4532(chlL) cce_4534(chlN)
CYP: 
CYC: 
CYN: 
CYH: 
CYJ: 
AMR: 
AM1_1444(chlN) AM1_1445(chlL) AM1_1539(chlB)
CGC: 
CAN: 
CSN: 
DSL: 
HAO: 
GLP: 
CMP: 
CYU: 
TER: 
LEP: 
GEI: 
OAC: 
ONI: 
PSEU: 
CEP: 
MIC: 
ARP: 
GVI: 
gvip017(chlB) gvip327(chlN) gvip328(chlL)
GLJ: 
GKIL_1146(chlB) GKIL_3933(chlN) GKIL_3934(chlL)
ANA: 
all5076(chlN) all5078(chlL) alr3441(chlB)
NPU: 
NOS: 
NOP: 
AVA: 
ANB: 
ACY: 
NAZ: 
CSG: 
CALO: 
CALT: 
RIV: 
PMA: 
Pro_0544(chlL) Pro_0545(chlB) Pro_0546(chlN)
PMM: 
PMM0543(chlL) PMM0544(chlB) PMM0545(chlN)
PMT: 
PMT1215(chlN) PMT1216(chlB) PMT1217(chlL)
PMN: 
PMI: 
PMB: 
PMC: 
PMF: 
PMG: 
PMH: 
PMJ: 
PME: 
PRC: 
PRM: 
CTHE: 
PLP: 
SCS: 
CTE: 
CT2150(chlL) CT2151(bchB) CT2152(bchN)
CPC: 
CCH: 
CPH: 
CPB: 
CLI: 
PVI: 
PLT: 
PPH: 
PAA: 
CTS: 
RRS: 
RCA: 
CAU: 
CAG: 
CHL: 
 » show all
Taxonomy
Reference
1  [PMID:8199775]
  Authors
Fujita Y, Matsumoto H, Takahashi Y, Matsubara H
  Title
Identification of a nifDK-like gene (ORF467) involved in the biosynthesis of chlorophyll in the cyanobacterium Plectonema boryanum.
  Journal
Plant. Cell. Physiol. 34 (1993) 305-14.
  Sequence
[up:Q04607]
Reference
2  [PMID:18358835]
  Authors
Nomata J, Ogawa T, Kitashima M, Inoue K, Fujita Y
  Title
NB-protein (BchN-BchB) of dark-operative protochlorophyllide reductase is the catalytic component containing oxygen-tolerant Fe-S clusters.
  Journal
FEBS. Lett. 582 (2008) 1346-50.
  Sequence
Reference
3  [PMID:20400946]
  Authors
Muraki N, Nomata J, Ebata K, Mizoguchi T, Shiba T, Tamiaki H, Kurisu G, Fujita Y
  Title
X-ray crystal structure of the light-independent protochlorophyllide reductase.
  Journal
Nature. 465 (2010) 110-4.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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