KEGG   ENZYME: 1.3.99.5Help
Entry
EC 1.3.99.5                 Enzyme                                 
Name
3-oxo-5alpha-steroid 4-dehydrogenase (acceptor);
steroid 5alpha-reductase;
3-oxosteroid Delta4-dehydrogenase;
3-oxo-5alpha-steroid Delta4-dehydrogenase;
steroid Delta4-5alpha-reductase;
Delta4-3-keto steroid 5alpha-reductase;
Delta4-3-oxo steroid reductase;
Delta4-3-ketosteroid5alpha-oxidoreductase;
Delta4-3-oxosteroid-5alpha-reductase;
3-keto-Delta4-steroid-5alpha-reductase;
5alpha-reductase;
testosterone 5alpha-reductase;
4-ene-3-ketosteroid-5alpha-oxidoreductase;
Delta4-5alpha-dehydrogenase;
3-oxo-5alpha-steroid:(acceptor) Delta4-oxidoreductase;
tesI (gene name)
Class
Oxidoreductases;
Acting on the CH-CH group of donors;
With other acceptors
BRITE hierarchy
Sysname
3-oxo-5alpha-steroid:acceptor Delta4-oxidoreductase
Reaction(IUBMB)
a 3-oxo-5alpha-steroid + acceptor = a 3-oxo-Delta4-steroid + reduced acceptor [RN:R02643]
Reaction(KEGG)
Substrate
3-oxo-5alpha-steroid [CPD:C02940];
acceptor [CPD:C00028]
Product
3-oxo-Delta4-steroid [CPD:C00619];
reduced acceptor [CPD:C00030]
Comment
A flavoprotein. This bacterial enzyme, characterized from Comamonas testosteroni, is involved in androsterone degradation. cf. EC 1.3.1.22, 3-oxo-5alpha-steroid 4-dehydrogenase (NADP+).
Pathway
Steroid degradation
Microbial metabolism in diverse environments
Orthology
K16051  
3-oxo-5alpha-steroid 4-dehydrogenase
Genes
PPUU: 
GPB: 
REH: 
CTI: 
CNC: 
CTT: 
NAR: 
NPP: 
SWI: 
SPHM: 
MPA: 
MAP1447c
MAV: 
MAV_3022
MIT: 
OCO_28580
MIR: 
OCQ_27160
MIA: 
OCU_28450
MID: 
MIP_03716
MSM: 
MSMEG_2873
MSG: 
MSMEI_2799
MSA: 
Mycsm_04160
MUL: 
MUL_4900
MVA: 
Mvan_2890
MGI: 
Mflv_4550 Mflv_4564
MSP: 
Mspyr1_39500 Mspyr1_39640
MAB: 
MAB_0623
MMV: 
MYCMA_0323(frdA)
MMC: 
Mmcs_0302 Mmcs_0316
MKM: 
Mkms_0312 Mkms_0326
MJL: 
Mjls_0293 Mjls_0307
MJD: 
JDM601_2019 JDM601_4251
MMI: 
MMAR_0205
MRH: 
MycrhN_4258
MMM: 
W7S_13850
MCB: 
Mycch_1589
MLI: 
MULP_00179
NFA: 
nfa30830 nfa4640 nfa46590
NCY: 
NOCYR_0491 NOCYR_3994 NOCYR_4393
NBR: 
O3I_002615 O3I_020135
RHA: 
RHA1_ro05698
RER: 
RER_07380 RER_33860 RER_43230
ROP: 
ROP_57660
REQ: 
REQ_07930 REQ_08850 REQ_12430 REQ_13730
TPR: 
Tpau_3846
TCU: 
Tcur_3515
FAL: 
FRAAL3038
AMD: 
AMED_2521
AMN: 
RAM_12815
AMM: 
AMES_2493
STP: 
Strop_2675
SAQ: 
Sare_2883
AMS: 
AMIS_19130
 » show all
Taxonomy
Reference
1  [PMID:13673006]
  Authors
LEVY HR, TALALAY P.
  Title
Bacterial oxidation of steroids. II. Studies on the enzymatic mechanism of ring A dehydrogenation.
  Journal
J. Biol. Chem. 234 (1959) 2014-21.
  Organism
Pseudomonas testosteroni
Reference
2  [PMID:8655514]
  Authors
Florin C, Kohler T, Grandguillot M, Plesiat P
  Title
Comamonas testosteroni 3-ketosteroid-delta 4(5 alpha)-dehydrogenase: gene and protein characterization.
  Journal
J. Bacteriol. 178 (1996) 3322-30.
Reference
3  [PMID:12902225]
  Authors
Horinouchi M, Hayashi T, Yamamoto T, Kudo T
  Title
A new bacterial steroid degradation gene cluster in Comamonas testosteroni TA441  which consists of aromatic-compound degradation genes for seco-steroids and 3-ketosteroid dehydrogenase genes.
  Journal
Appl. Environ. Microbiol. 69 (2003) 4421-30.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
9036-43-5
DBGET integrated database retrieval system