KEGG   ENZYME: 1.3.99.5Help
Entry
EC 1.3.99.5                 Enzyme                                 

Name
3-oxo-5alpha-steroid 4-dehydrogenase (acceptor);
steroid 5alpha-reductase;
3-oxosteroid Delta4-dehydrogenase;
3-oxo-5alpha-steroid Delta4-dehydrogenase;
steroid Delta4-5alpha-reductase;
Delta4-3-keto steroid 5alpha-reductase;
Delta4-3-oxo steroid reductase;
Delta4-3-ketosteroid5alpha-oxidoreductase;
Delta4-3-oxosteroid-5alpha-reductase;
3-keto-Delta4-steroid-5alpha-reductase;
5alpha-reductase;
testosterone 5alpha-reductase;
4-ene-3-ketosteroid-5alpha-oxidoreductase;
Delta4-5alpha-dehydrogenase;
3-oxo-5alpha-steroid:(acceptor) Delta4-oxidoreductase;
tesI (gene name)
Class
Oxidoreductases;
Acting on the CH-CH group of donors;
With unknown physiological acceptors
BRITE hierarchy
Sysname
3-oxo-5alpha-steroid:acceptor Delta4-oxidoreductase
Reaction(IUBMB)
a 3-oxo-5alpha-steroid + acceptor = a 3-oxo-Delta4-steroid + reduced acceptor [RN:R02643]
Reaction(KEGG)
Substrate
3-oxo-5alpha-steroid [CPD:C02940];
acceptor [CPD:C00028]
Product
3-oxo-Delta4-steroid [CPD:C00619];
reduced acceptor [CPD:C00030]
Comment
A flavoprotein. This bacterial enzyme, characterized from Comamonas testosteroni, is involved in androsterone degradation. cf. EC 1.3.1.22, 3-oxo-5alpha-steroid 4-dehydrogenase (NADP+).
History
EC 1.3.99.5 created 1965, modified 2012
Pathway
ec00984  Steroid degradation
ec01120  Microbial metabolism in diverse environments
Orthology
K16051  3-oxo-5alpha-steroid 4-dehydrogenase
Genes
PRE: PCA10_43670
PPUT: L483_14100
PPUN: PP4_27190
PPUU: PputUW4_01680
PKC: PKB_4321
PFZ: AV641_10570
PPSY: AOC04_07870
PTN: PTRA_a2599
PNG: PNIG_a2794
ZAL: AZF00_05200
GPB: HDN1F_19870(tesI)
REH: H16_B0641(h16_B0641)
BFN: OI25_4613
CTES: O987_07765
NAR: Saro_3721
SWI: Swit_1860
SPHM: G432_07425
SSAN: NX02_12730
MPA: MAP_1447c
MAO: MAP4_2397
MAVI: RC58_11915
MAVU: RE97_11925
MAV: MAV_3022
MAVD: NF84_13530
MAVR: LA63_13690
MAVA: LA64_13685
MIT: OCO_28580
MIA: OCU_28450
MIR: OCQ_27160
MID: MIP_03716
MYO: OEM_27040
MVA: Mvan_2890
MUL: MUL_4900
MAB: MAB_0623
MABB: MASS_0593
MABO: NF82_03145
MCHE: BB28_02985
MSTE: MSTE_00582
MMI: MMAR_0205
MMM: W7S_13850
NFR: ERS450000_00982(ifcA_1) ERS450000_03773(ifcA_3) ERS450000_05269(ifcA_4)
ROP: ROP_57660
TPR: Tpau_3846
SALU: DC74_8142
SALL: SAZ_42040
NDK: I601_0577(urdA)
PSIM: KR76_13710
TCU: Tcur_3515
FAL: FRAAL3038
AMD: AMED_2521
AMN: RAM_12815
AMM: AMES_2493
AMZ: B737_2494
AOI: AORI_2483
KAL: KALB_5139
ALL: CRK59602
SAQ: Sare_2883
 » show all
Taxonomy
Reference
1  [PMID:13673006]
  Authors
LEVY HR, TALALAY P.
  Title
Bacterial oxidation of steroids. II. Studies on the enzymatic mechanism of ring A dehydrogenation.
  Journal
J. Biol. Chem. 234 (1959) 2014-21.
Reference
2  [PMID:8655514]
  Authors
Florin C, Kohler T, Grandguillot M, Plesiat P
  Title
Comamonas testosteroni 3-ketosteroid-delta 4(5 alpha)-dehydrogenase: gene and protein characterization.
  Journal
J. Bacteriol. 178 (1996) 3322-30.
  Sequence
Reference
3  [PMID:12902225]
  Authors
Horinouchi M, Hayashi T, Yamamoto T, Kudo T
  Title
A new bacterial steroid degradation gene cluster in Comamonas testosteroni TA441  which consists of aromatic-compound degradation genes for seco-steroids and 3-ketosteroid dehydrogenase genes.
  Journal
Appl. Environ. Microbiol. 69 (2003) 4421-30.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.3.99.5
IUBMB Enzyme Nomenclature: 1.3.99.5
ExPASy - ENZYME nomenclature database: 1.3.99.5
BRENDA, the Enzyme Database: 1.3.99.5
CAS: 9036-43-5

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