KEGG   ENZYME: 1.7.2.1Help
Entry
EC 1.7.2.1                  Enzyme                                 

Name
nitrite reductase (NO-forming);
cd-cytochrome nitrite reductase;
[nitrite reductase (cytochrome)] [misleading, see comments.];
cytochrome c-551:O2, NO2+ oxidoreductase;
cytochrome cd;
cytochrome cd1;
hydroxylamine (acceptor) reductase;
methyl viologen-nitrite reductase;
nitrite reductase (cytochrome;
NO-forming)
Class
Oxidoreductases;
Acting on other nitrogenous compounds as donors;
With a cytochrome as acceptor
BRITE hierarchy
Sysname
nitric-oxide:ferricytochrome-c oxidoreductase
Reaction(IUBMB)
nitric oxide + H2O + ferricytochrome c = nitrite + ferrocytochrome c + 2 H+ [RN:R00783]
Reaction(KEGG)
Substrate
nitric oxide [CPD:C00533];
H2O [CPD:C00001];
ferricytochrome c [CPD:C00125]
Product
nitrite [CPD:C00088];
ferrocytochrome c [CPD:C00126];
H+ [CPD:C00080]
Comment
The reaction is catalysed by two types of enzymes, found in the perimplasm of denitrifying bacteria. One type comprises proteins containing multiple copper centres, the other a heme protein, cytochrome cd1. Acceptors include c-type cytochromes such as cytochrome c-550 or cytochrome c-551 from Paracoccus denitrificans or Pseudomonas aeruginosa, and small blue copper proteins such as azurin and pseudoazurin. Cytochrome cd1 also has oxidase and hydroxylamine reductase activities. May also catalyse the reaction of hydroxylamine reductase (EC 1.7.99.1) since this is a well-known activity of cytochrome cd1.
History
EC 1.7.2.1 created 1961, modified 1976, modified 2001, modified 2002 (EC 1.7.99.3 created 1961 as EC 1.6.6.5, transferred 1964 to EC 1.7.99.3, modified 1976, incorporated 2002, EC 1.9.3.2 created 1965, incorporated 2002)
Pathway
ec00910  Nitrogen metabolism
ec01120  Microbial metabolism in diverse environments
Orthology
K00368  nitrite reductase (NO-forming)
K15864  nitrite reductase (NO-forming) / hydroxylamine reductase
Genes
HPR: PARA_18490
MSU: MS0876(sufI)
APL: APL_2035(sufI)
APJ: APJL_2086(sufI2)
APA: APP7_2122(sufI)
ASU: Asuc_0978
ASI: ASU2_07220
ASS: ASU1_07295
AEU: ACEE_07505
GAN: UMN179_01765
SACZ: AOT14_33610(aniA)
PSUW: WQ53_05830
PAE: PA0519(nirS)
PAEV: N297_531(nirS)
PAEI: N296_531(nirS)
PAU: PA14_06750(nirS)
PAP: PSPA7_0620(nirS)
PAG: PLES_05161(nirS)
PAF: PAM18_0518(nirS)
PNC: NCGM2_5677(nirS)
PAEB: NCGM1900_0530(nirS)
PAEP: PA1S_02620
PAEM: U769_02645
PAEL: T223_02620
PAEU: BN889_00600(nirS)
PAEG: AI22_01285
PAEC: M802_530(nirS)
PAEO: M801_531(nirS)
PMK: MDS_0146
PCQ: PcP3B5_02670(nirK)
PFL: PFL_5501(nirK)
PPRC: PFLCHA0_c54550(nirK)
PPRO: PPC_5453
PFE: PSF113_3754(nirS)
PMAN: OU5_0457(nirS) OU5_P0207(nirS)
PEN: PSEEN5226
PSA: PST_3532(nirS)
PSZ: PSTAB_3504(nirS)
PSR: PSTAA_3629(nirS)
PSTT: CH92_04195
PSEM: TO66_27805
PSEC: CCOS191_0291(nirU)
PSOS: POS17_5442
PSIL: PMA3_16305
PSYC: DABAL43B_1588(aniA)
MCT: MCR_0135(aniA)
MCS: DR90_1762(nirK)
MCAT: MC25239_00170(aniA)
SDN: Sden_3482
SAZ: Sama_2681
SLO: Shew_3335
SWD: Swoo_1417
SMAV: CFF01_16430(nirK)
ILO: IL0762
CPS: CPS_4220(nirS)
PHA: PSHAa1477
PTN: PTRA_a1866(nirK)
PNG: PNIG_a1970(nirK)
MAQ: Maqu_3125
MHC: MARHY3064(nirS)
MBS: MRBBS_1013(nirK)
MEC: Q7C_88
TIG: THII_2875
NOC: Noc_0089
NHL: Nhal_1082
TEE: Tel_15465
TKM: TK90_1652
TVR: TVD_03855
HCH: HCH_04416
HCO: LOKO_01665(nirS_1)
ADI: B5T_01320
AXE: P40_06210
OCE: GU3_12660
SLIM: SCL_0264
SVA: SVA_0350
NMA: NMA1887
NME: NMB1623(pan1)
NMP: NMBB_1863
NMH: NMBH4476_0603(aniA)
NMC: NMC1549(aniA)
NMI: NMO_1452(aniA)
NMM: NMBM01240149_0554(nirK)
NMS: NMBM01240355_1553(aniA)
NMW: NMAA_1353(aniA)
NMZ: NMBNZ0533_1600(aniA)
NMX: NMA510612_2118(aniA)
NGO: NGO1276
NGK: NGK_1490
NLA: NLA_6490(aniA)
NWE: SAMEA3174300_1210(aniA)
NSI: A6J88_07705(nirK)
KKI: KKKWG1_1535(aniA)
CVI: CV_2007(aniA)
PSE: NH8B_1422(nirS)
RSO: RSp1503
RSL: RPSI07_3198(aniA)
RSN: RSPO_m00044(copA)
RSM: CMR15_mp30147(aniA)
RSE: F504_4973
RPI: Rpic_4015
REH: H16_B2277(nirS)
CNC: CNE_2c22450(nirS1)
RME: Rmet_3172(nirS)
CTI: RALTA_B2049(nirS)
BMAL: DM55_4646 DM55_4685(nirK)
BMAE: DM78_3373(nirK) DM78_3411
BMAQ: DM76_3599 DM76_3638(nirK)
BMAF: DM51_4456(nirK) DM51_4494
BMAZ: BM44_4638 BM44_4677(nirK)
BMAB: BM45_3181(nirK) BM45_3220
BPSE: BDL_4759 BDL_4797(nirK)
BPSM: BBQ_4659(nirK) BBQ_4696
BPSU: BBN_4897 BBN_4934(nirK)
BPSD: BBX_3921 BBX_3959(nirK)
BPK: BBK_4062 BBK_4100(nirK)
BPSH: DR55_3787(nirK) DR55_3823
BPSA: BBU_4548(nirK) BBU_4584
BPSO: X996_3573(nirK) X996_3610
BUT: X994_5630(nirK) X994_5666
BTQ: BTQ_4169(nirK) BTQ_4232
BTJ: BTJ_5201(nirK) BTJ_5270
BTZ: BTL_3671(nirK) BTL_3731
BTD: BTI_3972(nirK)
BTV: BTHA_4151 BTHA_4216(nirK)
BTHE: BTN_3926 BTN_5654(nirK)
BTHM: BTRA_3665(nirK) BTRA_3729
BTHL: BG87_3625(nirK) BG87_3688
BOK: DM82_5662(nirK)
BOC: BG90_4972(nirK)
BUL: BW21_5966(nirK)
BPT: Bpet4054(nirS)
AXY: AXYL_02390(nirK)
AXX: ERS451415_02178(nirK)
ACHR: C2U31_17265(nirK)
TEA: KUI_1530(nirK2)
TEG: KUK_0832(nirK2)
TAS: TASI_1505
TAT: KUM_0196(nirK2)
BPSI: IX83_07800
ODI: ODI_R3332
OUR: CEQ07_04645(nirK)
PNA: Pnap_1326
AJS: Ajs_1912
OTK: C6570_07175(nirK)
HAR: HEAR3245
JSV: CNX70_25620(nirK)
MASS: CR152_22600(nirK)
MASZ: C9I28_16400(nirK)
LCH: Lcho_1442
RGE: RGE_06580(nirS)
NEU: NE0924(aniA)
NET: Neut_1403
NLC: EBAPG3_001590(nirK) EBAPG3_003135(nirK)
SHD: SUTH_02586(nirS) SUTH_02890(nirS)
TBD: Tbd_0077
MMB: Mmol_1061
SLT: Slit_1129
SDR: SCD_n00366(napG) SCD_n02111(nirS)
EBA: ebA888(nirS)
AZA: AZKH_3548(nirS) AZKH_p0630(nirK_aniA)
TCL: Tchl_2591
NIS: NIS_1794
BBA: Bd2608
BBAT: Bdt_2523
BBW: BDW_09585
BBAC: EP01_09160
PLA: Plav_1009
SME: SMa1250(nirK)
SMI: BN406_04400(nirK)
SMEL: SM2011_a1250(nirK)
SMER: DU99_22655
SMD: Smed_6278
RHI: NGR_c09950(nirK)
SFH: SFHH103_00930(nirK)
SFD: USDA257_c38590(nirK)
SIX: BSY16_4428(nirK)
SAME: SAMCFNEI73_Ch1322(nirK)
EAD: OV14_b0141(nirK)
ATU: Atu4382(nirK)
AGC: BSY240_110(nirK) BSY240_80(nirK)
RET: RHE_PF00525(nirK)
REL: REMIM1_PF00193(nirK)
RIR: BN877_p0528(nirK)
RGA: RGR602_PC02109(nirK)
RHN: AMJ98_PB00153(nirK)
RPHA: AMC79_PA00191(nirK)
RHT: NT26_0628 NT26_3653(nirK)
RHX: AMK02_PB00167(nirK)
NGL: RG1141_CH34420(nirK)
NGG: RG540_CH35240(nirK)
SHZ: shn_16520
BME: BMEII0988
BMEL: DK63_2268(nirK)
BMI: BMEA_B0261(nirK)
BMZ: BM28_B0251(nirK)
BMG: BM590_B0251(nirK)
BMEE: DK62_3139(nirK)
BMF: BAB2_0943
BABO: DK55_2887(nirK)
BABR: DO74_2202(nirK)
BABT: DK49_2352(nirK)
BABB: DK48_2910(nirK)
BABU: DK53_2890(nirK)
BABS: DK51_2375(nirK)
BABC: DO78_2167(nirK)
BMS: BRA0260
BSUI: BSSP1_II0222(nirK)
BSUP: BSPT1_II0222(nirK)
BSUV: BSPT2_II0223(nirK)
BSUC: BSSP2_II0227(nirK)
BMT: BSUIS_B0265(nirK)
BSZ: DK67_2279(nirK)
BOV: BOV_A0236(nirK)
BCS: BCAN_B0261(nirK)
BCAR: DK60_3132(nirK)
BCAS: DA85_11745
BMR: BMI_II254(nirK)
BPP: BPI_II255(nirK)
BPV: DK65_2218(nirK)
BVL: BF3285c2_0930(nirK)
OAH: DR92_3865(nirK) DR92_659(nirK)
OCH: CES85_5169(nirK)
BJA: blr7089(nirK)
BRA: BRADO1227(nirK)
BBT: BBta_6826(nirK)
BRS: S23_12320(nirK)
AOL: S58_13390(nirK) S58_68210
BRAD: BF49_4443
BRO: BRAD285_0763(nirK)
RPA: RPA3306(nirK1) RPA4145(nirK2)
RPE: RPE_4071
NWI: Nwi_2648
NHA: Nham_3281
OCG: OCA5_c08660(aniA1) OCA5_c25200(aniA2)
OCO: OCA4_c08650(aniA1) OCA4_c25190(aniA2)
SNO: Snov_1147
MSL: Msil_1519
HDN: Hden_0591
DEI: C4375_00345(nirK)
RBM: TEF_02750
CSE: Cseg_3038
PZU: PHZ_c0614
SIL: SPOA0220(nirS)
RDE: RD1_1565(nirS)
RLI: RLO149_c031380(nirS)
PDE: Pden_2487
DSH: Dshi_3180(nirS)
PSF: PSE_0898(nirS)
PGA: PGA1_262p01260(nirK)
PGL: PGA2_239p1440(nirK)
PGD: Gal_03862
PPIC: PhaeoP14_03617(nirK)
CID: P73_0921
SPSE: SULPSESMR1_04749(nirK)
AHT: ANTHELSMS3_03928(nirK)
SAGU: CDO87_23285(nirK)
SWI: Swit_1793
SPHD: HY78_16620
SPHC: CVN68_17600(nirK)
SPHT: K426_03035
MGY: MGMSRv2__1403(nirS)
MAGX: XM1_0428(nirS) XM1_2022(nirS)
ALI: AZOLI_p30082(nirK)
ABS: AZOBR_p310167(aniA)
TXI: TH3_16160
THAC: CSC3H3_22140(nirK)
PGV: SL003B_0576(nirS)
GKA: GK0767
GTN: GTNG_0650(nirK)
STH: STH741
TMR: Tmar_1506
SAY: TPY_3652
LPIL: LIP_3346
MID: MIP_02743
MIR: OCQ_18170
MVA: Mvan_3626
MGI: Mflv_3489
CEF: CE0272
CDI: DIP1876
CDIP: ERS451417_01876(aniA_2) ERS451417_01877(aniA_3)
CUR: cu0227
CPL: Cp3995_0569(aniA)
CPG: Cp316_0577(aniA)
CPP: CpP54B96_0569(aniA)
CPK: Cp1002_0560(aniA)
CPQ: CpC231_0563(aniA)
CPX: CpI19_0562(aniA)
CPZ: CpPAT10_0562(aniA)
COR: Cp267_0584(aniA)
COP: Cp31_0566(aniA)
COD: Cp106_0545(aniA)
COS: Cp4202_0554(aniA)
COI: CpCIP5297_0569(aniA)
COE: Cp258_0563(aniA)
COU: Cp162_0556(aniA)
CPSE: CPTA_01110
CPSU: CPTB_00423
CPSF: CPTC_00082
COA: DR71_1140
CSP: WM42_0304
CPHO: CPHO_07585
JDE: Jden_0150
XCE: Xcel_1641
CFL: Cfla_1768
CFI: Celf_1338
SERJ: SGUI_1956
PAC: PPA0092
PACC: PAC1_00475
PACH: PAGK_0092
CACN: RN83_00890
PFR: PFREUD_03790(aniA)
MPH: MLP_11520
NCA: Noca_2101
TFU: Tfu_2794
SRO: Sros_4252
ACE: Acel_0503
BSD: BLASA_4885(aniA)
AMI: Amir_4752
MIL: ML5_4465
BAST: BAST_1175
BCOR: BCOR_1023
SIJ: SCIP_0489
PDO: PSDT_0983
TBI: Tbis_2351
RCA: Rcas_3430
CAU: Caur_1570
CAG: Cagg_1796
HAU: Haur_1295
STI: Sthe_0153
ATM: ANT_27710(nirS)
TBC: A0O31_00427(aniA)
TTR: Tter_2149
OTE: Oter_1019
OBG: Verru16b_00368(aniA)
MIN: Minf_0929(sufI)
LBI: LEPBI_I1063(aniA)
LBF: LBF_1028
GAU: GAU_2766(nirK)
GBA: J421_0402
SMIZ: 4412673_03220(aniA)
GRS: C7S20_04845(nirK) C7S20_18960(nirK)
FJO: Fjoh_2418
CAPN: CBG49_08855(nirK)
CGH: CGC50_01910(nirK)
CLK: CGC53_00800(nirK)
CSPU: CGC55_00410(nirK)
LACI: CW733_03040(nirK)
POA: CW731_01540(nirK)
MPW: MPR_3003
AALG: AREALGSMS7_01838(aniA)
FEK: C1H87_09905(nirK)
FBA: FIC_00388
FBU: UJ101_02130(nirK)
HTH: HTH_0150(nirS)
NDE: NIDE2534 NIDE4252(nirK)
NIO: NITINOP_3146(nirK)
MOX: DAMO_2415(nirS)
HHB: Hhub_2693(nirK)
HMA: rrnAC2853(nirK)
HHI: HAH_0129(nirK)
NPH: NP_1598A(nirK)
HUT: Huta_0035
HMU: Hmuk_1016
HVO: HVO_2141(nirK)
HME: HFX_2183(aniA)
SRUB: C2R22_01020(nirK) C2R22_12205(nirK)
HTU: Htur_3087
SALI: L593_03900
ACJ: ACAM_0018
NGA: Ngar_c04260(nirK) Ngar_c23400(nirK1)
NVN: NVIE_000530(nirK)
NEV: NTE_01403
TAA: NMY3_00439(aniA_1)
NBV: T478_1026
 » show all
Taxonomy
Reference
1  [PMID:5354021]
  Authors
Miyata M, Mori T.
  Title
Studies on denitrification. X. The "denitrifying enzyme" as a nitrite reductase and the electron donating system for denitrification.
  Journal
J. Biochem. (Tokyo). 66 (1969) 463-71.
Reference
2  [PMID:13295215]
  Authors
CHUNG CW, NAJJAR VA.
  Title
Cofactor requirements for enzymatic denitrification.  I.  Nitrite reductase.
  Journal
J. Biol. Chem. 218 (1956) 617-25.
Reference
3  [PMID:13782716]
  Authors
WALKER GC, NICHOLAS DJ.
  Title
Nitrite reductase from Pseudomonas aeruginosa.
  Journal
Biochim. Biophys. Acta. 49 (1961) 350-60.
Reference
4
  Authors
Singh, J.
  Title
Cytochrome oxidase from Pseudomonas aeruginosa. III. Reduction of hydroxylamine.
  Journal
Biochim. Biophys. Acta 333 (1974) 28-36.
Reference
5
  Authors
Michalski, W.P. and Nicholas, D.J.D.
  Title
Molecular characterization of a copper-containing nitrite reductase from Rhodopseudomonas sphaeriodes forma sp. Denitrificans.
  Journal
Biochim. Biophys. Acta 828 (1985) 130-137.
Reference
6  [PMID:1862344]
  Authors
Godden JW, Turley S, Teller DC, Adman ET, Liu MY, Payne WJ, LeGall J.
  Title
The 2.3 angstrom X-ray structure of nitrite reductase from Achromobacter cycloclastes.
  Journal
Science. 253 (1991) 438-42.
  Sequence
Reference
7  [PMID:7583671]
  Authors
Williams PA, Fulop V, Leung YC, Chan C, Moir JW, Howlett G, Ferguson SJ, Radford SE, Hajdu J.
  Title
Pseudospecific docking surfaces on electron transfer proteins as illustrated by pseudoazurin, cytochrome c550 and cytochrome cd1 nitrite reductase.
  Journal
Nat. Struct. Biol. 2 (1995) 975-82.
Reference
8  [PMID:8639023]
  Authors
Hole UH, Vollack KU, Zumft WG, Eisenmann E, Siddiqui RA, Friedrich B, Kroneck PM.
  Title
Characterization of the membranous denitrification enzymes nitrite reductase (cytochrome cd1) and copper-containing nitrous oxide reductase from Thiobacillus denitrificans.
  Journal
Arch. Microbiol. 165 (1996) 55-61.
  Sequence
[tbd:Tbd_0077]
Reference
9  [PMID:9409151]
  Authors
Zumft WG.
  Title
Cell biology and molecular basis of denitrification.
  Journal
Microbiol. Mol. Biol. Rev. 61 (1997) 533-616.
  Sequence
Reference
10 [PMID:9667932]
  Authors
Ferguson SJ.
  Title
Nitrogen cycle enzymology.
  Journal
Curr. Opin. Chem. Biol. 2 (1998) 182-93.
Reference
11 [PMID:9308169]
  Authors
Vijgenboom E, Busch JE, Canters GW.
  Title
In vivo studies disprove an obligatory role of azurin in denitrification in Pseudomonas aeruginosa and show that azu expression is under control of rpoS and ANR.
  Journal
Microbiology. 143 ( Pt 9) (1997) 2853-63.
Other DBs
ExplorEnz - The Enzyme Database: 1.7.2.1
IUBMB Enzyme Nomenclature: 1.7.2.1
ExPASy - ENZYME nomenclature database: 1.7.2.1
UM-BBD (Biocatalysis/Biodegradation Database): 1.7.2.1
BRENDA, the Enzyme Database: 1.7.2.1
CAS: 9080-03-9

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