KEGG   ENZYME: 1.7.3.1Help
Entry
EC 1.7.3.1                  Enzyme                                 

Name
nitroalkane oxidase;
nitroethane oxidase;
NAO;
nitroethane:oxygen oxidoreductase
Class
Oxidoreductases;
Acting on other nitrogenous compounds as donors;
With oxygen as acceptor
BRITE hierarchy
Sysname
nitroalkane:oxygen oxidoreductase
Reaction(IUBMB)
a nitroalkane + H2O + O2 = an aldehyde or ketone + nitrite + H2O2 [RN:R10388 R10389]
Reaction(KEGG)
Substrate
nitroalkane [CPD:C06058];
H2O [CPD:C00001];
O2 [CPD:C00007]
Product
aldehyde [CPD:C00071];
ketone [CPD:C01450];
nitrite [CPD:C00088];
H2O2 [CPD:C00027]
Comment
Has an absolute requirement for FAD [4]. While nitroethane may be the physiological substrate [2], the enzyme also acts on several other nitroalkanes, including 1-nitropropane, 2-nitropropane, 1-nitrobutane, 1-nitropentane, 1-nitrohexane, nitrocyclohexane and some nitroalkanols [4]. Differs from EC 1.13.11.16, nitronate monooxygenase, in that the preferred substrates are neutral nitroalkanes rather than anionic nitronates [4].
History
EC 1.7.3.1 created 1961, modified 2006, modified 2009
Pathway
Nitrogen metabolism
Reference
1  [PMID:14907722]
  Authors
LITTLE HN.
  Title
Oxidation of nitroethane by extracts from Neurospora.
  Journal
J. Biol. Chem. 193 (1951) 347-58.
Reference
2  [PMID:22538]
  Authors
Kido T, Hashizume K, Soda K.
  Title
Purification and properties of nitroalkane oxidase from Fusarium oxysporum.
  Journal
J. Bacteriol. 133 (1978) 53-8.
  Sequence
[up:Q8X1D8]
Reference
3  [PMID:11867731]
  Authors
Daubner SC, Gadda G, Valley MP, Fitzpatrick PF.
  Title
Cloning of nitroalkane oxidase from Fusarium oxysporum identifies a new member of the acyl-CoA dehydrogenase superfamily.
  Journal
Proc. Natl. Acad. Sci. U. S. A. 99 (2002) 2702-7.
  Sequence
[up:Q8X1D8]
Reference
4  [PMID:15581574]
  Authors
Fitzpatrick PF, Orville AM, Nagpal A, Valley MP.
  Title
Nitroalkane oxidase, a carbanion-forming flavoprotein homologous to acyl-CoA dehydrogenase.
  Journal
Arch. Biochem. Biophys. 433 (2005) 157-65.
Reference
5  [PMID:15713081]
  Authors
Valley MP, Tichy SE, Fitzpatrick PF.
  Title
Establishing the kinetic competency of the cationic imine intermediate in nitroalkane oxidase.
  Journal
J. Am. Chem. Soc. 127 (2005) 2062-6.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
UM-BBD (Biocatalysis/Biodegradation Database): 
BRENDA, the Enzyme Database: 
CAS: 
9029-36-1 65802-82-6

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