KEGG   ENZYME: 1.7.3.3Help
Entry
EC 1.7.3.3                  Enzyme                                 

Name
factor-independent urate hydroxylase;
uric acid oxidase;
uricase;
uricase II;
urate oxidase
Class
Oxidoreductases;
Acting on other nitrogenous compounds as donors;
With oxygen as acceptor
BRITE hierarchy
Sysname
urate:oxygen oxidoreductase
Reaction(IUBMB)
urate + O2 + H2O = 5-hydroxyisourate + H2O2 [RN:R02106]
Reaction(KEGG)
R02106;
(other) R07981
Show
Substrate
urate [CPD:C00366];
O2 [CPD:C00007];
H2O [CPD:C00001]
Product
5-hydroxyisourate [CPD:C11821];
H2O2 [CPD:C00027]
Comment
This enzyme was previously thought to be a copper protein, but it is now known that the enzymes from soy bean (Glycine max), the mould Aspergillus flavus and Bacillus subtilis contains no copper nor any other transition-metal ion. The 5-hydroxyisourate formed decomposes spontaneously to form allantoin and CO2, although there is an enzyme-catalysed pathway in which EC 3.5.2.17, hydroxyisourate hydrolase, catalyses the first step. The enzyme is different from EC 1.14.13.113 (FAD-dependent urate hydroxylase).
History
EC 1.7.3.3 created 1961, modified 2002, modified 2005, modified 2010
Pathway
ec00230  Purine metabolism
ec00232  Caffeine metabolism
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K00365  urate oxidase
K16838  urate oxidase / 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase
Genes
GGO: 101139849
NLE: 100597726
MCC: 714004(Uox)
MCF: 102117077(Uox)
CSAB: 103224536
RRO: 104677513
RBB: 108527687
CJC: 100397154
SBQ: 101040936
MMU: 22262(Uox)
RNO: 114768(Uox)
CGE: 100768251
NGI: 103728098
HGL: 101719675
CCAN: 109688705
OCU: 100009266(UOX)
TUP: 102500418
CFA: 490189(UOX)
AML: 100470134
UMR: 103662970
ORO: 101366343
FCA: 101096750
PTG: 102971100
AJU: 106973351
BTA: 514113(UOX)
BOM: 102267915
BIU: 109556136
PHD: 102330860
CHX: 102168228
OAS: 101109838
SSC: 397510(UOX)
CFR: 102505313
CDK: 105095038(uricase)
BACU: 103009966
LVE: 103070467
OOR: 101275718
ECB: 100064919
EPZ: 103557286
EAI: 106824012
MYB: 102244414
MYD: 102751767
PALE: 102878680
LAV: 100668450
TMU: 101357598
MDO: 100012925
SHR: 100917431
OAA: 100078972
GGA: 101747367
MGP: 100551010
CJO: 107317543
APLA: 101797537
ACYG: 106033995
TGU: 100189932(UOX)
GFR: 102040716
FAB: 101806442
PHI: 102109233
PMAJ: 107208239
CCW: 104695300
FPG: 101917753
FCH: 102059654
CLV: 102096622
EGZ: 104122902
AAM: 106499688
ASN: 102373915
AMJ: 102571917(UOX)
CMY: 102932244(Uricase) 102932468(Uricase)
ACS: 100552598
PVT: 110079741
PBI: 103058911
GJA: 107117302
XLA: 108714977 399031(MGC68684)
XTR: 496896(uox)
NPR: 108791380
DRE: 436604(uox)
SRX: 107742901
SGH: 107560424
CCAR: 109098556
IPU: 100528126(uric)
AMEX: 103030682
TRU: 101078943
LCO: 104931943(Uricase)
NCC: 104941345
MZE: 101475077
OLA: 101159660
XMA: 102230976
PRET: 103464157
NFU: 107392713
CSEM: 103392251
LCF: 108881276
HCQ: 109522038
BPEC: 110160092
SASA: 100136464
ELS: 105008143
SFM: 108923494
LCM: 102360643
CMK: 103174706
SPU: 588974
SKO: 100368654
DME: Dmel_CG7171(Uro)
DPO: Dpse_GA20153(Dpse_Uro)
DSI: Dsimw501_GD23486(Dsim_GD23486)
DVI: Dvir_GJ10793(Dvir_Uro) Dvir_GJ26133
MDE: 101898809
AAG: 5573896
SOC: 105195412
AEC: 105151023
ACEP: 105619788
PBAR: 105424989
CFO: 105250801
NVI: 100123613
NVL: 108565014
BMOR: 692784
PMAC: 106714268
PRAP: 110994260
PXY: 105386323
FCD: 110846031
CRG: 105344267
MYI: 110442106
EPA: 110235296
ADF: 107344624
ATH: AT2G26230
CRB: 17894740
BRP: 103828734
BOE: 106301645
CPAP: 110814496
CIT: 102631080
TCC: 18586835
GRA: 105779785
DZI: 111296600
EGR: 104421156
GMX: 100037445(UOX) 547453(N-35)
VRA: 106770791
VAR: 108326630
CCAJ: 109815339
CAM: 101500535(nodulin-35)
LJA: Lj6g3v0143890.1(Lj6g3v0143890.1)
FVE: 101304417
PPER: 18778286
PMUM: 103330981
PAVI: 110758511
PXB: 103933611
ZJU: 107413131
CSV: 101206975
CMO: 103489372
MCHA: 111018658
RCU: 8272393
JCU: 105649139
JRE: 109002171
VVI: 100232988
SLY: 100037732
SPEN: 107002894
SOT: 102581180
CANN: 107851994
NSY: 104211356
NTO: 104091371
INI: 109147930
SIND: 105161011
OEU: 111375160
HAN: 110925667
LSV: 111916296
DCR: 108205953
BVG: 104907803
SOE: 110804304
OSA: 4324793
DOSA: Os01t0865100-01(Os01g0865100)
OBR: 102704100
BDI: 100845980
ATS: 109743862(LOC109743862)
SBI: 110433901
ZMA: 100274529
SITA: 101785812
PDA: 103721005
MUS: 103979093
AOF: 109836810
ATR: 18444423
TDL: TDEL_0F02670(TDEL0F02670)
PIC: PICST_40781(URO1)
CAL: CAALFM_C200180CA(CaO19.2114)
CAUR: QG37_07102
NCR: NCU07853
NTE: NEUTE1DRAFT62263(NEUTE1DRAFT_62263)
MGR: MGG_05283
MAW: MAC_01658
MAJ: MAA_02492
CMT: CCM_09432
MBE: MBM_07067
ANI: AN9470.2
ANG: ANI_1_1150034(An03g02330) ANI_1_826024(An02g06030)
ABE: ARB_05777
TVE: TRV_04465
PTE: PTT_12595
CNE: CNI02420
CNB: CNBH2300
ABP: AGABI1DRAFT42333(AGABI1DRAFT_42333)
ABV: AGABI2DRAFT65743(AGABI2DRAFT_65743)
DDI: DDB_G0286427(uox)
DFA: DFA_04065(uox)
SPAR: SPRG_09685
SCL: sce4023
SME: SM_b21284
VGO: GJW-30_1_02473(uox)
SNO: Snov_3388
MET: M446_5249
MNO: Mnod_5813
MOR: MOC_0208(pucL)
MEE: CS521_04795(pucL)
BSU: BSU32450(pucL)
BSR: I33_3345(pucL)
BSL: A7A1_2459
BSH: BSU6051_32450(pucL)
BSUT: BSUB_03463(pucL)
BSUL: BSUA_03463(pucL)
BSUS: Q433_17750
BSS: BSUW23_15825(pucL)
BST: GYO_3538(pucL)
BSO: BSNT_09727(pucL)
BSQ: B657_32450(pucL)
BSX: C663_3103(pucL)
BHA: BH0759
BCL: ABC3735
BJS: MY9_3258
BACY: QF06_14540
BACL: BS34A_35410(pucL)
BALM: BsLM_3241
BEO: BEH_09915
BBEV: BBEV_2689(uao)
BKO: CKF48_06250(uraD)
GGH: GHH_c21820(pucL)
GEA: GARCT_02054(uao)
BSE: Bsel_0582
PMS: KNP414_03236(uao)
PMW: B2K_17880
PSWU: SY83_16740
BTS: Btus_2019
MAB: MAB_2929c
MABB: MASS_2864
MABO: NF82_14655
MCHE: BB28_14755
MSTE: MSTE_02898
ASD: AS9A_4198
NFA: NFA_52420
NFR: ERS450000_04639(uox)
NTP: CRH09_09400(pucL) CRH09_28995(pucL)
RER: RER_21140
REY: O5Y_10090
RFA: A3L23_00088(uox)
RHS: A3Q41_03326(uox)
RQI: C1M55_10755(pucL)
SCO: SCO6211(SC2G5.32)
SMA: SAVERM_2018(pucL)
SGR: SGR_1309
SGB: WQO_28725
SCT: SCAT_4779
SFA: Sfla_1052
SBH: SBI_02950
SHY: SHJG_7271
SDV: BN159_2098(uox)
SALB: XNR_0633
SALS: SLNWT_0615
STRP: F750_5793
SFI: SFUL_6171
SALU: DC74_6400
SALL: SAZ_32150
STRE: GZL_02614
SLD: T261_1848
SAMB: SAM23877_5951(uox)
SPRI: SPRI_1552
SRW: TUE45_06834(uox)
SLE: sle_14680(sle_14680)
STRD: NI25_08290
SMAL: SMALA_6025
SLAU: SLA_6161
SALJ: SMD11_1431
SLX: SLAV_08750(uox1) SLAV_19850(uox2)
KSK: KSE_63370
CMC: CMN_00267
CRY: B7495_07110(pucL)
ART: Arth_3427
ARR: ARUE_c35390(uox)
ARN: CGK93_19080(pucL)
AAU: AAur_3402
ACH: Achl_3208
KRH: KRH_19780
BRV: CFK39_12195(pucL)
BRZ: CFK38_15305(pucL)
LMOI: VV02_25695
CFL: Cfla_2783
SERJ: SGUI_2186
BLIN: BLSMQ_0835
MPH: MLP_22140
NCA: Noca_1142
NDA: Ndas_0718
NAL: B005_3625(pucL)
NGV: CDO52_20165(pucL)
SRO: Sros_2773
FAL: FRAAL4508
GOB: Gobs_2838
MMAR: MODMU_0113(uox)
KRA: Krad_2071
SEN: SACE_6736
AMD: AMED_1926
AMN: RAM_09770
AMM: AMES_1910
AMZ: B737_1912
AOI: AORI_5946
AJA: AJAP_09695(uox)
AMYC: CU254_07830(pucL) CU254_30640(pucL)
PDX: Psed_1766
PSEA: WY02_01130
PSEE: FRP1_13340
PSEH: XF36_13125
AMI: Amir_5939
APRE: CNX65_29160(pucL)
SESP: BN6_71160(uox)
KAL: KALB_4480
ALL: CRK55966
ACTN: L083_6156
AFS: AFR_31535
CAI: Caci_4737
SNA: Snas_2538
TBI: Tbis_2592
AEY: CDG81_19595(pucL)
RXY: Rxyl_2843
DRA: DR_1160
DGE: Dgeo_2601
TRA: Trad_1503
SACI: Sinac_4646
SUS: Acid_0359
ABAC: LuPra_02263(pucL_1)
CPI: Cpin_6768
NMO: Nmlp_3624(pucL1)
HWC: Hqrw_2223(pucL1)
HVO: HVO_B0300
 » show all
Taxonomy
Reference
1  [PMID:13363909]
  Authors
HUDSON PB, LONDON M.
  Title
Purification and properties of solubilized uricase.
  Journal
Biochim. Biophys. Acta. 21 (1956) 290-8.
Reference
2  [PMID:13271340]
  Authors
MAHLER HR, HUBSCHER G, BAUM R.
  Title
Studies on uricase.  I.  Preparation, purification, and properties of a cuproprotein.
  Journal
J. Biol. Chem. 216 (1955) 625-41.
Reference
3  [PMID:13252004]
  Authors
ROBBINS KC, BARNETT EL, GRANT NH.
  Title
Partial pruficiation of procine liver uricase.
  Journal
J. Biol. Chem. 216 (1955) 27-35.
Reference
4  [PMID:9709003]
  Authors
Kahn K, Tipton PA.
  Title
Spectroscopic characterization of intermediates in the urate oxidase reaction.
  Journal
Biochemistry. 37 (1998) 11651-9.
Reference
5  [PMID:9360612]
  Authors
Colloc'h N, el Hajji M, Bachet B, L'Hermite G, Schiltz M, Prange T, Castro B, Mornon JP.
  Title
Crystal structure of the protein drug urate oxidase-inhibitor complex at 2.05 A resolution.
  Journal
Nat. Struct. Biol. 4 (1997) 947-52.
  Sequence
Reference
6  [PMID:12680763]
  Authors
Imhoff RD, Power NP, Borrok MJ, Tipton PA.
  Title
General base catalysis in the urate oxidase reaction: evidence for a novel Thr-Lys catalytic diad.
  Journal
Biochemistry. 42 (2003) 4094-100.
Other DBs
ExplorEnz - The Enzyme Database: 1.7.3.3
IUBMB Enzyme Nomenclature: 1.7.3.3
ExPASy - ENZYME nomenclature database: 1.7.3.3
UM-BBD (Biocatalysis/Biodegradation Database): 1.7.3.3
BRENDA, the Enzyme Database: 1.7.3.3
CAS: 9002-12-4

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