| Entry |
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| Name |
factor-independent urate hydroxylase;
uric acid oxidase;
uricase;
uricase II;
urate oxidase
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| Class |
Oxidoreductases;
Acting on other nitrogenous compounds as donors;
With oxygen as acceptor
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| Sysname |
urate:oxygen oxidoreductase
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| Reaction(IUBMB) |
urate + O2 + H2O = 5-hydroxyisourate + H2O2 [RN: R02106]
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| Reaction(KEGG) |
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| Substrate |
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| Product |
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| Comment |
This enzyme was previously thought to be a copper protein, but it is now known that the enzymes from soy bean (Glycine max), the mould Aspergillus flavus and Bacillus subtilis contains no copper nor any other transition-metal ion. The 5-hydroxyisourate formed decomposes spontaneously to form allantoin and CO2, although there is an enzyme-catalysed pathway in which EC 3.5.2.17, hydroxyisourate hydrolase, catalyses the first step. The enzyme is different from EC 1.14.13.113 (FAD-dependent urate hydroxylase).
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| Pathway |
| Purine metabolism | | Caffeine metabolism | | Metabolic pathways | | Microbial metabolism in diverse environments |
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| Orthology |
| urate oxidase | | urate oxidase / 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase |
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| Genes |
PTR: | | PPS: | | GGO: | | MCC: | | MMU: | | RNO: | | CFA: | | AML: | | FCA: | | BTA: | | SSC: | | ECB: | | MDO: | | SHR: | | OAA: | | MGP: | | TGU: | | ACS: | | XLA: | | XTR: | | DRE: | | TRU: | | OLA: | | BFO: | | SPU: | | DME: | | DPO: | | DAN: | | DER: | | DPE: | | DSE: | | DSI: | | DWI: | | DYA: | | DGR: | | DMO: | | DVI: | | AGA: | | AAG: | | CQU: | | NVI: | | NVE: | | ATH: | | ALY: | | GMX: | | CSV: | | RCU: | | POP: | | VVI: | | OSA: | | DOSA: | | BDI: | | ZMA: | | SMO: | | PPP: | | CRE: | | VCN: | | KLA: | | LTH: | | PPA: | | ZRO: | | TDL: | | DHA: | | PIC: | | PGU: | | LEL: | | CAL: | | CTP: | | CDU: | | COT: | | YLI: | | CLU: | | NCR: | | SMP: | | PAN: | | TTT: | | MTM: | | MGR: | | FGR: | | NHE: | | VAL: | | SSL: | | BFU: | | ANI: | | NFI: | | AFM: | | AOR: | | ANG: | | AFV: | | ACT: | | PCS: | | CIM: | | CPW: | | PBL: | | URE: | | ABE: | | TVE: | | AJE: | | PNO: | | PTE: | | ZTR: | | TML: | | SPO: | | CNE: | | CNB: | | CGI: | | LBC: | | MPR: | | CCI: | | UMA: | | PGR: | | NGR: | | DDI: | | DPP: | | PTI: | | PIF: | | SCL: | | SME: | | SNO: | | MRD: | | MET: | | MNO: | | BSU: | | BSR: | | BSL: | | BSS: | | BST: | | BSN: | | BSQ: | | BSUB: | | BHA: | | BCL: | | BSE: | | BJS: | | GGH: | | PJD: | | PMS: | | PMQ: | | PMW: | | BTS: | | MSM: | | MSG: | | MAB: | | MMV: | | ASD: | | NFA: | | NBR: | | RHA: | | RER: | | ROP: | | SCO: | | SMA: | | SGR: | | SCB: | | SSX: | | SVL: | | SCT: | | SCY: | | SFA: | | SBH: | | SHY: | | SHO: | | SVE: | | SDV: | | SALB: | | STRP: | | KSK: | | CMC: | | ART: | | AAU: | | ACH: | | AAI: | | APN: | | ARR: | | KRH: | | BFA: | | CFL: | | MPH: | | NCA: | | NDA: | | NAL: | | SRO: | | FRA: | | FRE: | | FRI: | | FAL: | | FSY: | | NML: | | GOB: | | MMAR: | | KRA: | | SEN: | | TBI: | | AMD: | | AMN: | | AMM: | | PDX: | | AMI: | | SESP: | | AMS: | | CAI: | | SNA: | | RXY: | | ACM: | | GMA: | | TSA: | | TRS: | | SUS: | | CPI: | | NKO: | | SACI: | | PHM: | | DRA: | | DGE: | | DGO: | | DPD: | | TRA: | | HWC: | | NMO: | | HVO: | | HJE: | | NOU: | |
» show all
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| Reference |
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| Authors |
HUDSON PB, LONDON M. |
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| Title |
Purification and properties of solubilized uricase. |
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| Journal |
Biochim. Biophys. Acta. 21 (1956) 290-8. |
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| Organism |
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| Reference |
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| Authors |
MAHLER HR, HUBSCHER G, BAUM R. |
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| Title |
Studies on uricase. I. Preparation, purification, and properties of a cuproprotein. |
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| Journal |
J. Biol. Chem. 216 (1955) 625-41. |
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| Organism |
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| Reference |
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| Authors |
ROBBINS KC, BARNETT EL, GRANT NH. |
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| Title |
Partial pruficiation of procine liver uricase. |
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| Journal |
J. Biol. Chem. 216 (1955) 27-35. |
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| Organism |
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| Reference |
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| Authors |
Kahn K, Tipton PA. |
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| Title |
Spectroscopic characterization of intermediates in the urate oxidase reaction. |
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| Journal |
Biochemistry. 37 (1998) 11651-9. |
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| Organism |
Glycine max |
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| Reference |
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| Authors |
Colloc'h N, el Hajji M, Bachet B, L'Hermite G, Schiltz M, Prange T, Castro B, Mornon JP. |
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| Title |
Crystal structure of the protein drug urate oxidase-inhibitor complex at 2.05 A resolution. |
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| Journal |
Nat. Struct. Biol. 4 (1997) 947-52. |
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| Organism |
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| Reference |
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| Authors |
Imhoff RD, Power NP, Borrok MJ, Tipton PA. |
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| Title |
General base catalysis in the urate oxidase reaction: evidence for a novel Thr-Lys catalytic diad. |
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| Journal |
Biochemistry. 42 (2003) 4094-100. |
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| Organism |
Bacillus subtilis [GN: bsu] |
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| Other DBs |
ExplorEnz - The Enzyme Database: IUBMB Enzyme Nomenclature: ExPASy - ENZYME nomenclature database: UM-BBD (Biocatalysis/Biodegradation Database): BRENDA, the Enzyme Database: CAS: 9002-12-4 |
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