KEGG   ENZYME: 2.1.1.156Help
Entry
EC 2.1.1.156                Enzyme                                 

Name
glycine/sarcosine N-methyltransferase;
ApGSMT;
glycine-sarcosine methyltransferase;
GSMT;
GMT;
glycine sarcosine N-methyltransferase;
S-adenosyl-L-methionine:sarcosine N-methyltransferase
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
BRITE hierarchy
Sysname
S-adenosyl-L-methionine:glycine(or sarcosine) N-methyltransferase [sarcosine(or N,N-dimethylglycine)-forming]
Reaction(IUBMB)
2 S-adenosyl-L-methionine + glycine = 2 S-adenosyl-L-homocysteine + N,N-dimethylglycine (overall reaction) [RN:R10060];
(1a) S-adenosyl-L-methionine + glycine = S-adenosyl-L-homocysteine + sarcosine [RN:R00367];
(1b) S-adenosyl-L-methionine + sarcosine = S-adenosyl-L-homocysteine + N,N-dimethylglycine [RN:R07243]
Reaction(KEGG)
Substrate
S-adenosyl-L-methionine [CPD:C00019];
glycine [CPD:C00037];
sarcosine [CPD:C00213]
Product
S-adenosyl-L-homocysteine [CPD:C00021];
N,N-dimethylglycine [CPD:C01026];
sarcosine [CPD:C00213]
Comment
Cells of the oxygen-evolving halotolerant cyanobacterium Aphanocthece halophytica synthesize betaine from glycine by a three-step methylation process. This is the first enzyme and it leads to the formation of either sarcosine or N,N-dimethylglycine, which is further methylated to yield betaine (N,N,N-trimethylglycine) by the action of EC 2.1.1.157, sarcosine/dimethylglycine N-methyltransferase. Differs from EC 2.1.1.20, glycine N-methyltransferase, as it can further methylate the product of the first reaction. Acetate, dimethylglycine and S-adenosyl-L-homocysteine can inhibit the reaction [3].
History
EC 2.1.1.156 created 2005
Pathway
ec00260  Glycine, serine and threonine metabolism
Orthology
K18896  glycine/sarcosine N-methyltransferase
Genes
PFS: PFLU_2095
PFW: PF1751_v1c19160
PFF: PFLUOLIPICF727140
PFX: A7318_08950
PTV: AA957_04385
PAZO: AYR47_17170
TIG: THII_1900 THII_1901
TMB: Thimo_1798 Thimo_2027
MPUR: MARPU_03465
TEE: Tel_06780
TGR: Tgr7_1181
TKM: TK90_0179
TVR: TVD_00875
HOH: Hoch_6748
PLA: Plav_2856
AAY: WYH_00219
MAGQ: MGMAQ_3234
MMI: MMAR_0861
SEN: SACE_3901 SACE_5862(gsmT)
SNA: Snas_2966
SYW: SYNW1914(gsmt)
SYNR: KR49_11730
SYNW: SynWH8103_02187(gbmt1)
PMT: PMT_0552
CTS: Ctha_2188
MMH: Mmah_0524
 » show all
Taxonomy
Reference
1  [PMID:10896953]
  Authors
Nyyssola A, Kerovuo J, Kaukinen P, von Weymarn N, Reinikainen T.
  Title
Extreme halophiles synthesize betaine from glycine by methylation.
  Journal
J. Biol. Chem. 275 (2000) 22196-201.
  Sequence
Reference
2  [PMID:11319079]
  Authors
Nyyssola A, Reinikainen T, Leisola M.
  Title
Characterization of glycine sarcosine N-methyltransferase and sarcosine dimethylglycine N-methyltransferase.
  Journal
Appl. Environ. Microbiol. 67 (2001) 2044-50.
  Sequence
Reference
3  [PMID:12466265]
  Authors
Waditee R, Tanaka Y, Aoki K, Hibino T, Jikuya H, Takano J, Takabe T, Takabe T.
  Title
Isolation and functional characterization of N-methyltransferases that catalyze betaine synthesis from glycine in a halotolerant photosynthetic organism Aphanothece halophytica.
  Journal
J. Biol. Chem. 278 (2003) 4932-42.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.156
IUBMB Enzyme Nomenclature: 2.1.1.156
ExPASy - ENZYME nomenclature database: 2.1.1.156
BRENDA, the Enzyme Database: 2.1.1.156
CAS: 294210-82-5

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