KEGG   ENZYME: 2.1.1.169Help
Entry
EC 2.1.1.169                Enzyme                                 

Name
tricetin 3',4',5'-O-trimethyltransferase;
FOMT;
TaOMT1;
TaCOMT1;
TaOMT2
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
BRITE hierarchy
Sysname
S-adenosyl-L-methionine:tricetin 3',4',5'-O-trimethyltransferase
Reaction(IUBMB)
3 S-adenosyl-L-methionine + tricetin = 3 S-adenosyl-L-homocysteine + 3',4',5'-O-trimethyltricetin (overall reaction) [RN:R09679];
(1a) S-adenosyl-L-methionine + tricetin = S-adenosyl-L-homocysteine + 3'-O-methyltricetin [RN:R09612];
(1b) S-adenosyl-L-methionine + 3'-O-methyltricetin = S-adenosyl-L-homocysteine + 3',5'-O-dimethyltricetin [RN:R09613];
(1c) S-adenosyl-L-methionine + 3',5'-O-dimethyltricetin = S-adenosyl-L-homocysteine + 3',4',5'-O-trimethyltricetin [RN:R09678]
Reaction(KEGG)
Substrate
S-adenosyl-L-methionine [CPD:C00019];
tricetin [CPD:C10192];
3'-O-methyltricetin [CPD:C19739];
3',5'-O-dimethyltricetin [CPD:C10193]
Product
S-adenosyl-L-homocysteine [CPD:C00021];
3',4',5'-O-trimethyltricetin [CPD:C19807];
3'-O-methyltricetin [CPD:C19739];
3',5'-O-dimethyltricetin [CPD:C10193]
Comment
The enzyme from Triticum aestivum catalyses the sequential O-methylation of tricetin via 3'-O-methyltricetin, 3',5'-O-methyltricetin to 3',4',5'-O-trimethyltricetin [2].
History
EC 2.1.1.169 created 2010
Reference
1  [PMID:18397325]
  Authors
Kornblatt JA, Zhou JM, Ibrahim RK
  Title
Structure-activity relationships of wheat flavone O-methyltransferase: a homodimer of convenience.
  Journal
FEBS. J. 275 (2008) 2255-66.
  Sequence
[up:Q38J50]
Reference
2  [PMID:16730127]
  Authors
Zhou JM, Gold ND, Martin VJ, Wollenweber E, Ibrahim RK
  Title
Sequential O-methylation of tricetin by a single gene product in wheat.
  Journal
Biochim. Biophys. Acta. 1760 (2006) 1115-24.
  Sequence
[up:Q38J50]
Reference
3  [PMID:19211254]
  Authors
Zhou JM, Seo YW, Ibrahim RK
  Title
Biochemical characterization of a putative wheat caffeic acid O-methyltransferase.
  Journal
Plant. Physiol. Biochem. 47 (2009) 322-6.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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