methylenetetrahydrofolate---tRNA-(uracil54-C5)-methyltransferase (FADH2-oxidizing);
folate-dependent ribothymidyl synthase;
methylenetetrahydrofolate-transfer ribonucleate uracil 5-methyltransferase;
5,10-methylenetetrahydrofolate:tRNA-UPsiC (uracil-5-)-methyl-transferase;
5,10-methylenetetrahydrofolate:tRNA (uracil-5-)-methyl-transferase;
TrmFO;
folate/FAD-dependent tRNA T54 methyltransferase

Transferases;
Transferring one-carbon groups;
Methyltransferases

5,10-methylenetetrahydrofolate:tRNA (uracil54-C5)-methyltransferase

5,10-methylenetetrahydrofolate + uridine54 in tRNA + FADH2 = tetrahydrofolate + 5-methyluridine54 in tRNA + FAD [RN:R03704]

R03704

5,10-methylenetetrahydrofolate [CPD:C00143];
uridine54 in tRNA;
FADH2 [CPD:C01352]

Up to 25% of the bases in mature tRNA are post-translationally modified or hypermodified. One almost universal post-translational modification is the conversion of U54 into ribothymidine in the TPsiC loop, and this modification is found in most species studied to date [2]. Unlike this enzyme, which uses 5,10-methylenetetrahydrofolate and FADH2 to supply the atoms for methylation of U54, EC 2.1.1.35, tRNA (uracil54-C5)-methyltransferase, uses S-adenosyl-L-methionine.

1  [PMID:6768721]

Delk AS, Nagle DP Jr, Rabinowitz JC.

Methylenetetrahydrofolate-dependent biosynthesis of ribothymidine in transfer RNA of Streptococcus faecalis. Evidence for reduction of the 1-carbon unit by FADH2.

J. Biol. Chem. 255 (1980) 4387-90.

Streptococcus faecalis

2  [PMID:9417931]

Becker HF, Motorin Y, Sissler M, Florentz C, Grosjean H.

Major identity determinants for enzymatic formation of ribothymidine and pseudouridine in the T psi-loop of yeast tRNAs.

J. Mol. Biol. 274 (1997) 505-18.

Saccharomyces cerevisiae [GN:sce]

3  [PMID:19416846]

Nishimasu H, Ishitani R, Yamashita K, Iwashita C, Hirata A, Hori H, Nureki O

Atomic structure of a folate/FAD-dependent tRNA T54 methyltransferase.

Proc. Natl. Acad. Sci. U. S. A. 106 (2009) 8180-5.