KEGG   ENZYME: 2.1.1.86Help
Entry
EC 2.1.1.86                 Enzyme                                 

Name
tetrahydromethanopterin S-methyltransferase;
tetrahydromethanopterin methyltransferase;
mtrA-H (gene names);
cmtA (gene name);
N5-methyltetrahydromethanopterin---coenzyme M methyltransferase;
5-methyl-5,6,7,8-tetrahydromethanopterin:2-mercaptoethanesulfonate 2-methyltransferase
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
BRITE hierarchy
Sysname
5-methyl-5,6,7,8-tetrahydromethanopterin:CoM 2-methyltransferase (Na+-transporting)
Reaction(IUBMB)
5-methyl-5,6,7,8-tetrahydromethanopterin + CoM + 2 Na+in = 5,6,7,8-tetrahydromethanopterin + 2-(methylsulfanyl)ethane-1-sulfonate + 2 Na+out [RN:R04347]
Reaction(KEGG)
Substrate
5-methyl-5,6,7,8-tetrahydromethanopterin [CPD:C04488];
CoM [CPD:C03576];
Na+ [CPD:C01330]
Product
5,6,7,8-tetrahydromethanopterin [CPD:C01217];
2-(methylsulfanyl)ethane-1-sulfonate [CPD:C03920];
Na+ [CPD:C01330]
Comment
Involved in the formation of methane from CO2 in methanogenic archaea. The reaction involves the export of one or two sodium ions. The enzyme from the archaeon Methanobacterium thermoautotrophicum is a membrane-associated multienzyme complex composed of eight different subunits, and contains a 5'-hydroxybenzimidazolyl-cobamide prosthetic group, to which the methyl group is attached during the transfer. A soluble enzyme that is induced by the presence of CO has been reported as well [6].
History
EC 2.1.1.86 created 1989, modified 2000, modified 2017
Pathway
ec00680  Methane metabolism
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K00577  tetrahydromethanopterin S-methyltransferase subunit A
K00578  tetrahydromethanopterin S-methyltransferase subunit B
K00579  tetrahydromethanopterin S-methyltransferase subunit C
K00580  tetrahydromethanopterin S-methyltransferase subunit D
K00581  tetrahydromethanopterin S-methyltransferase subunit E
K00582  tetrahydromethanopterin S-methyltransferase subunit F
K00583  tetrahydromethanopterin S-methyltransferase subunit G
K00584  tetrahydromethanopterin S-methyltransferase subunit H
Genes
TEE: Tel_11320
SVA: SVA_2695
DAR: Daro_2280
DTI: Desti_0878 Desti_5438 Desti_5449
MCH: Mchl_5727
HMC: HYPMC_2275
CID: P73_3963
MAG: amb1203
PKU: AUO94_00060
CACE: CACET_c34490(mtrH)
DSY: DSY3157
DHD: Dhaf_4327
AWO: Awo_c07520(mttA2)
TOC: Toce_1535
ACA: ACP_1207
LFC: LFE_1243
MMP: MMP1560(mtrE) MMP1561(mtrD) MMP1562(mtrC) MMP1563(mtrB) MMP1564(mtrA) MMP1565(or900) MMP1566(mtrG) MMP1567(mtrH)
MAC: MA_0269(mtrH) MA_0270(mtrG) MA_0271(mtrF) MA_0272(mtrA) MA_0273(mtrB) MA_0274(mtrC) MA_0275(mtrD) MA_0276(mtrE) MA_1804(mtxH) MA_1805(mtxA)
MBU: Mbur_1518(mtrH) Mbur_1519(mtrG) Mbur_1520(mtrF) Mbur_1521(mtrA) Mbur_1522(mtrB) Mbur_1523(mtrC) Mbur_1524(mtrD) Mbur_1525(mtrE) Mbur_2244
MCJ: MCON_1068(mtrH) MCON_1069(mtrG) MCON_1070(mtrF) MCON_1071(mtrA) MCON_1072(mtrB) MCON_1073(mtrC) MCON_1074(mtrD) MCON_1075(mtrE) MCON_2872 MCON_2873
MBG: BN140_1739(mtrE) BN140_1740(mtrD) BN140_1741(mtrC) BN140_1742(mtrB) BN140_1743(mtrA1) BN140_1744(mtrF) BN140_1745(mtrA2) BN140_1746(mtrH)
MPD: MCP_0539(mtrE) MCP_0540(mtrD) MCP_0541(mtrC) MCP_0542(mtrB) MCP_0543(mtrA-1) MCP_0544(mtrF) MCP_0545(mtrG) MCP_0546(mtrH) MCP_0678(mtrA-2)
MEZ: Mtc_0936(mtrE) Mtc_0937(mtrD) Mtc_0938(mtrC) Mtc_0939(mtrB) Mtc_0940(mtrA-1) Mtc_0941(mtrF) Mtc_0942(mtrG) Mtc_0943(mtrH) Mtc_0962(mtrA-2)
RCI: RCIX2064(mtrE) RCIX2065(mtrD) RCIX2066(mtrC) RCIX2067(mtrB) RCIX2068(mtrA-1) RCIX2069(mtrA-2) RCIX2070(mtrG) RCIX2071(mtrH)
MWO: MWSIV6_1448(mtrA2) MWSIV6_1548(mtrH) MWSIV6_1549(mtrG) MWSIV6_1550(mtrF) MWSIV6_1551(mtrA1) MWSIV6_1552(mtrB) MWSIV6_1553(mtrC) MWSIV6_1554(mtrD) MWSIV6_1555(mtrE)
MST: Msp_0300(mtrE) Msp_0301(mtrD) Msp_0302(mtrC) Msp_0303(mtrB) Msp_0304(mtrA) Msp_0305(mtrF) Msp_0306(mtrG) Msp_0307(mtrH)
MRU: mru_0441(mtrA2) mru_1916(mtrH) mru_1917(mtrG) mru_1918(mtrF) mru_1919(mtrA1) mru_1920(mtrB) mru_1921(mtrC) mru_1922(mtrD) mru_1923(mtrE)
MEB: Abm4_1518(mtrH) Abm4_1519(mtrG) Abm4_1520(mtrF) Abm4_1521(mtrA) Abm4_1522(mtrB) Abm4_1523(mtrC) Abm4_1524(mtrD) Abm4_1525(mtrE)
MMIL: sm9_0659(mtrA2) sm9_1915(mtrH2) sm9_2019(mtrH1) sm9_2020(mtrG) sm9_2021(mtrF) sm9_2022(mtrA1) sm9_2023(mtrB) sm9_2024(mtrC) sm9_2025(mtrD) sm9_2026(mtrE)
MFC: BRM9_0927(mtrH) BRM9_0928(mtrG) BRM9_0929(mtrF) BRM9_0930(mtrA1) BRM9_0931(mtrB) BRM9_0932(mtrC) BRM9_0933(mtrD) BRM9_0934(mtrE) BRM9_1713(mtrA2) BRM9_2057(mtrA3)
MCUB: MCBB_1144(mtrA2) MCBB_1977(mtrH) MCBB_1978(mtrG) MCBB_1979(mtrF) MCBB_1980(mtrA1) MCBB_1981(mtrB) MCBB_1982(mtrC) MCBB_1983(mtrD) MCBB_1984(mtrE)
MKA: MK0656(mtrE) MK0657(mtrD) MK0658(mtrC) MK0659(mtrB) MK0660(mtrA) MK0661(mtrG) MK0662(mtrH) MK1485(mtrF)
GAC: GACE_1353
GAH: GAH_01245
HALH: HTSR_1745(mtrH)
HHSR: HSR6_1811(mtrH)
SMR: Smar_0413
IAG: Igag_0398
NMR: Nmar_1747
NCT: NMSP_1666(mtrA1)
NEV: NTE_01720
NCV: NCAV_0248
NDV: NDEV_2077
KCR: Kcr_0196
BARC: AOA65_0679(mtrH_1) AOA65_1010(mtrH_2) AOA65_2047(mtrH_3)
LOKI: Lokiarch_10270(mtrH_1) Lokiarch_42890(mtrH_2) Lokiarch_51490(mtrH_3)
 » show all
Taxonomy
Reference
1  [PMID:3085670]
  Authors
Sauer FD.
  Title
Tetrahydromethanopterin methyltransferase, a component of the methane synthesizing complex of Methanobacterium thermoautotrophicum.
  Journal
Biochem. Biophys. Res. Commun. 136 (1986) 542-7.
Reference
2  [PMID:8477726]
  Authors
Gartner P, Ecker A, Fischer R, Linder D, Fuchs G, Thauer RK
  Title
Purification and properties of N5-methyltetrahydromethanopterin:coenzyme M methyltransferase from Methanobacterium thermoautotrophicum.
  Journal
Eur. J. Biochem. 213 (1993) 537-45.
Reference
3  [PMID:7813469]
  Authors
Weiss DS, Gartner P, Thauer RK
  Title
The energetics and sodium-ion dependence of N5-methyltetrahydromethanopterin:coenzyme M methyltransferase studied with cob(I)alamin as methyl acceptor and methylcob(III)alamin as methyl donor.
  Journal
Eur. J. Biochem. 226 (1994) 799-809.
Reference
4  [PMID:7737157]
  Authors
Harms U, Weiss DS, Gartner P, Linder D, Thauer RK
  Title
The energy conserving N5-methyltetrahydromethanopterin:coenzyme M methyltransferase complex from Methanobacterium thermoautotrophicum is composed of eight different subunits.
  Journal
Eur. J. Biochem. 228 (1995) 640-8.
Reference
5  [PMID:11248186]
  Authors
Gottschalk G, Thauer RK
  Title
The Na(+)-translocating methyltransferase complex from methanogenic archaea.
  Journal
Biochim. Biophys. Acta. 1505 (2001) 28-36.
Reference
6  [PMID:22636775]
  Authors
Vepachedu VR, Ferry JG
  Title
Role of the fused corrinoid/methyl transfer protein CmtA during CO-dependent growth of Methanosarcina acetivorans.
  Journal
J. Bacteriol. 194 (2012) 4161-8.
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.86
IUBMB Enzyme Nomenclature: 2.1.1.86
ExPASy - ENZYME nomenclature database: 2.1.1.86
UM-BBD (Biocatalysis/Biodegradation Database): 2.1.1.86
BRENDA, the Enzyme Database: 2.1.1.86
CAS: 103406-60-6

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