KEGG   ENZYME: 2.1.1.86Help
Entry
EC 2.1.1.86                 Enzyme                                 

Name
tetrahydromethanopterin S-methyltransferase;
tetrahydromethanopterin methyltransferase;
mtrA-H (gene names);
cmtA (gene name);
N5-methyltetrahydromethanopterin---coenzyme M methyltransferase;
5-methyl-5,6,7,8-tetrahydromethanopterin:2-mercaptoethanesulfonate 2-methyltransferase
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
BRITE hierarchy
Sysname
5-methyl-5,6,7,8-tetrahydromethanopterin:coenzyme-M 2-methyltransferase (Na+-transporting)
Reaction(IUBMB)
5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M + 2 Na+in = 5,6,7,8-tetrahydromethanopterin + 2-(methylsulfanyl)ethane-1-sulfonate + 2 Na+out [RN:R04347]
Reaction(KEGG)
Substrate
5-methyl-5,6,7,8-tetrahydromethanopterin [CPD:C04488];
coenzyme M [CPD:C03576];
Na+ [CPD:C01330]
Product
5,6,7,8-tetrahydromethanopterin [CPD:C01217];
2-(methylsulfanyl)ethane-1-sulfonate [CPD:C03920];
Na+ [CPD:C01330]
Comment
Involved in the formation of methane from CO2 in methanogenic archaea. The reaction involves the export of one or two sodium ions. The enzyme from the archaeon Methanobacterium thermoautotrophicum is a membrane-associated multienzyme complex composed of eight different subunits, and contains a 5'-hydroxybenzimidazolyl-cobamide prosthetic group, to which the methyl group is attached during the transfer. A soluble enzyme that is induced by the presence of CO has been reported as well [6].
History
EC 2.1.1.86 created 1989, modified 2000, modified 2017
Pathway
Methane metabolism
Metabolic pathways
Microbial metabolism in diverse environments
Orthology
K00577  
tetrahydromethanopterin S-methyltransferase subunit A
K00578  
tetrahydromethanopterin S-methyltransferase subunit B
K00579  
tetrahydromethanopterin S-methyltransferase subunit C
K00580  
tetrahydromethanopterin S-methyltransferase subunit D
K00581  
tetrahydromethanopterin S-methyltransferase subunit E
K00582  
tetrahydromethanopterin S-methyltransferase subunit F
K00583  
tetrahydromethanopterin S-methyltransferase subunit G
K00584  
tetrahydromethanopterin S-methyltransferase subunit H
Genes
TEE: 
DAR: 
DTI: 
MCH: 
HMC: 
CID: 
MAG: 
PKU: 
CACE: 
GFE: 
DSY: 
DHD: 
DDH: 
DKU: 
TJR: 
DOR: 
DMI: 
DED: 
DEC: 
DRS: 
AWO: 
Awo_c07520(mttA2)
TOC: 
ACA: 
NIA: 
LFC: 
LFI: 
MJA: 
MFE: 
MVU: 
MFS: 
MIF: 
MJH: 
MIG: 
MMP: 
MMP1560(mtrE) MMP1561(mtrD) MMP1562(mtrC) MMP1563(mtrB) MMP1564(mtrA) MMP1565(or900) MMP1566(mtrG) MMP1567(mtrH)
MMQ: 
MMX: 
MMZ: 
MMD: 
MMAK: 
MMAO: 
MAE: 
MVN: 
MVO: 
MOK: 
MAC: 
MA_0269(mtrH) MA_0270(mtrG) MA_0271(mtrF) MA_0272(mtrA) MA_0273(mtrB) MA_0274(mtrC) MA_0275(mtrD) MA_0276(mtrE) MA_1804(mtxH) MA_1805(mtxA)
MBA: 
MBY: 
MBW: 
MBAR: 
MBAK: 
MMA: 
MMAZ: 
MMJ: 
MMAC: 
MVC: 
MEK: 
MLS: 
METM: 
MEF: 
MEQ: 
MSJ: 
MSZ: 
MSW: 
MTHE: 
MTHR: 
MHOR: 
MBU: 
Mbur_1518(mtrH) Mbur_1519(mtrG) Mbur_1520(mtrF) Mbur_1521(mtrA) Mbur_1522(mtrB) Mbur_1523(mtrC) Mbur_1524(mtrD) Mbur_1525(mtrE) Mbur_2244
MMET: 
MMH: 
MHAZ: 
MEV: 
MZH: 
MPY: 
MHZ: 
MTP: 
MCJ: 
MHI: 
MHU: 
MLA: 
MEM: 
MBG: 
BN140_1739(mtrE) BN140_1740(mtrD) BN140_1741(mtrC) BN140_1742(mtrB) BN140_1743(mtrA1) BN140_1744(mtrF) BN140_1745(mtrA2) BN140_1746(mtrH)
MEMA: 
MPI: 
MBN: 
MFO: 
MPL: 
MPD: 
MCP_0539(mtrE) MCP_0540(mtrD) MCP_0541(mtrC) MCP_0542(mtrB) MCP_0543(mtrA-1) MCP_0544(mtrF) MCP_0545(mtrG) MCP_0546(mtrH) MCP_0678(mtrA-2)
MEZ: 
Mtc_0936(mtrE) Mtc_0937(mtrD) Mtc_0938(mtrC) Mtc_0939(mtrB) Mtc_0940(mtrA-1) Mtc_0941(mtrF) Mtc_0942(mtrG) Mtc_0943(mtrH) Mtc_0962(mtrA-2)
RCI: 
RCIX2064(mtrE) RCIX2065(mtrD) RCIX2066(mtrC) RCIX2067(mtrB) RCIX2068(mtrA-1) RCIX2069(mtrA-2) RCIX2070(mtrG) RCIX2071(mtrH)
MTH: 
MMG: 
METC: 
MWO: 
MST: 
Msp_0300(mtrE) Msp_0301(mtrD) Msp_0302(mtrC) Msp_0303(mtrB) Msp_0304(mtrA) Msp_0305(mtrF) Msp_0306(mtrG) Msp_0307(mtrH)
MSI: 
MRU: 
mru_0441(mtrA2) mru_1916(mtrH) mru_1917(mtrG) mru_1918(mtrF) mru_1919(mtrA1) mru_1920(mtrB) mru_1921(mtrC) mru_1922(mtrD) mru_1923(mtrE)
MEB: 
Abm4_1518(mtrH) Abm4_1519(mtrG) Abm4_1520(mtrF) Abm4_1521(mtrA) Abm4_1522(mtrB) Abm4_1523(mtrC) Abm4_1524(mtrD) Abm4_1525(mtrE)
MMIL: 
sm9_0659(mtrA2) sm9_1915(mtrH2) sm9_2019(mtrH1) sm9_2020(mtrG) sm9_2021(mtrF) sm9_2022(mtrA1) sm9_2023(mtrB) sm9_2024(mtrC) sm9_2025(mtrD) sm9_2026(mtrE)
MEYE: 
MOL: 
MEL: 
MEW: 
METH: 
MFC: 
BRM9_0927(mtrH) BRM9_0928(mtrG) BRM9_0929(mtrF) BRM9_0930(mtrA1) BRM9_0931(mtrB) BRM9_0932(mtrC) BRM9_0933(mtrD) BRM9_0934(mtrE) BRM9_1713(mtrA2) BRM9_2057(mtrA3)
MFI: 
MCUB: 
MCBB_1144(mtrA2) MCBB_1977(mtrH) MCBB_1978(mtrG) MCBB_1979(mtrF) MCBB_1980(mtrA1) MCBB_1981(mtrB) MCBB_1982(mtrC) MCBB_1983(mtrD) MCBB_1984(mtrE)
MFV: 
MKA: 
MK0656(mtrE) MK0657(mtrD) MK0658(mtrC) MK0659(mtrB) MK0660(mtrA) MK0661(mtrG) MK0662(mtrH) MK1485(mtrF)
AFG: 
AST: 
FPL: 
GAC: 
GAH: 
HALH: 
HTSR_1745(mtrH)
HHSR: 
HSR6_1811(mtrH)
SMR: 
SHC: 
IAG: 
THG: 
NMR: 
NIR: 
NID: 
NIN: 
NKR: 
NCT: 
NMSP_1666(mtrA1)
CSY: 
NGA: 
NVN: 
NEV: 
TAA: 
TAH: 
NDV: 
KCR: 
BARC: 
AOA65_0679(mtrH_1) AOA65_1010(mtrH_2) AOA65_2047(mtrH_3)
LOKI: 
 » show all
Taxonomy
Reference
1  [PMID:3085670]
  Authors
Sauer FD.
  Title
Tetrahydromethanopterin methyltransferase, a component of the methane synthesizing complex of Methanobacterium thermoautotrophicum.
  Journal
Biochem. Biophys. Res. Commun. 136 (1986) 542-7.
Reference
2  [PMID:8477726]
  Authors
Gartner P, Ecker A, Fischer R, Linder D, Fuchs G, Thauer RK
  Title
Purification and properties of N5-methyltetrahydromethanopterin:coenzyme M methyltransferase from Methanobacterium thermoautotrophicum.
  Journal
Eur. J. Biochem. 213 (1993) 537-45.
Reference
3  [PMID:7813469]
  Authors
Weiss DS, Gartner P, Thauer RK
  Title
The energetics and sodium-ion dependence of N5-methyltetrahydromethanopterin:coenzyme M methyltransferase studied with cob(I)alamin as methyl acceptor and methylcob(III)alamin as methyl donor.
  Journal
Eur. J. Biochem. 226 (1994) 799-809.
Reference
4  [PMID:7737157]
  Authors
Harms U, Weiss DS, Gartner P, Linder D, Thauer RK
  Title
The energy conserving N5-methyltetrahydromethanopterin:coenzyme M methyltransferase complex from Methanobacterium thermoautotrophicum is composed of eight different subunits.
  Journal
Eur. J. Biochem. 228 (1995) 640-8.
Reference
5  [PMID:11248186]
  Authors
Gottschalk G, Thauer RK
  Title
The Na(+)-translocating methyltransferase complex from methanogenic archaea.
  Journal
Biochim. Biophys. Acta. 1505 (2001) 28-36.
Reference
6  [PMID:22636775]
  Authors
Vepachedu VR, Ferry JG
  Title
Role of the fused corrinoid/methyl transfer protein CmtA during CO-dependent growth of Methanosarcina acetivorans.
  Journal
J. Bacteriol. 194 (2012) 4161-8.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
UM-BBD (Biocatalysis/Biodegradation Database): 
BRENDA, the Enzyme Database: 
CAS: 
103406-60-6

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