KEGG   ENZYME: 2.4.1.186Help
Entry
EC 2.4.1.186                Enzyme                                 

Name
glycogenin glucosyltransferase;
glycogenin;
priming glucosyltransferase;
UDP-glucose:glycogenin glucosyltransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
UDP-alpha-D-glucose:glycogenin alpha-D-glucosyltransferase
Reaction(IUBMB)
UDP-alpha-D-glucose + glycogenin = UDP + alpha-D-glucosylglycogenin [RN:R03681]
Reaction(KEGG)
Substrate
UDP-alpha-D-glucose [CPD:C00029];
glycogenin [CPD:C01702]
Product
UDP [CPD:C00015];
alpha-D-glucosylglycogenin [CPD:C02687]
Comment
The first reaction of this enzyme is to catalyse its own glucosylation, normally at Tyr-194 of the protein if this group is free. When Tyr-194 is replaced by Thr or Phe, the enzyme's Mn2+-dependent self-glucosylation activity is lost but its intermolecular transglucosylation ability remains [7]. It continues to glucosylate an existing glucosyl group until a length of about 5--13 residues has been formed. Further lengthening of the glycogen chain is then carried out by EC 2.4.1.11, glycogen (starch) synthase. The enzyme is not highly specific for the donor, using UDP-xylose in addition to UDP-glucose (although not glucosylating or xylosylating a xylosyl group so added). It can also use CDP-glucose and TDP-glucose, but not ADP-glucose or GDP-glucose. Similarly it is not highly specific for the acceptor, using water (i.e. hydrolysing UDP-glucose) among others. Various forms of the enzyme exist, and different forms predominate in different organs. Thus primate liver contains glycogenin-2, of molecular mass 66 kDa, whereas the more widespread form is glycogenin-1, with a molecular mass of 38 kDa.
History
EC 2.4.1.186 created 1992 (EC 2.4.1.112 created 1984, incorporated 2007)
Orthology
K00750  glycogenin
Genes
HSA: 2992(GYG1) 8908(GYG2)
PTR: 460765(GYG1) 465469(GYG2)
PPS: 100977408(GYG1) 100995852(GYG2)
GGO: 101130219(GYG1) 101153384(GYG2)
PON: 100431369(GYG2) 100434629(GYG1)
NLE: 100579312(GYG1) 100598771(GYG2)
MCC: 712495(GYG1) 720568(GYG2)
MCF: 101926679(GYG1) 102135191 102136979(GYG2)
CSAB: 103231541(GYG2) 103241506(GYG1)
RRO: 104671556(GYG1) 104678275(GYG2)
RBB: 108518298(GYG1) 108537905(GYG2)
CJC: 100405072(GYG1) 100407906(GYG2) 108589838
SBQ: 101048137(GYG2) 101053597(GYG1)
MMU: 27357(Gyg)
RNO: 81675(Gyg1)
CGE: 100751671(Gyg1)
NGI: 103745990(Gyg1)
HGL: 101709254(Gyg2) 101716513(Gyg1)
CCAN: 109682828(Gyg1) 109698210(Gyg2)
OCU: 100009058(GYG1)
TUP: 102473120(GYG1) 102475805(GYG2)
CFA: 491717(GYG2) 611614(GYG1)
AML: 100468752(GYG2) 100473212(GYG1)
UMR: 103666302(GYG2) 103681483(GYG1)
ORO: 101375485(GYG1) 101384616(GYG2)
FCA: 101080946(GYG2) 101082176(GYG1)
PTG: 102959321(GYG2) 102968386(GYG1)
AJU: 106975172(GYG1) 106982065(GYG2)
BTA: 280812(GYG1) 505258(GYG2)
BOM: 102265731(GYG1) 102267728(GYG2)
BIU: 109555288(GYG2) 109563118(GYG1)
PHD: 102332563(GYG2) 102338836(GYG1)
CHX: 102175944(GYG2) 102184201(GYG1)
OAS: 101103289(GYG1) 101115765(GYG2)
SSC: 100620160(GYG1) 102163050(GYG2)
CFR: 102509684(GYG1) 102514856(GYG2)
CDK: 105089507(GYG1) 105097369(GYG2)
BACU: 103001146(GYG1) 103003273(GYG2)
LVE: 103082327(GYG2) 103089765(GYG1)
OOR: 101289834(GYG1) 101289938(GYG2)
ECB: 100058811(GYG1) 100064536(GYG2)
EPZ: 103558755(GYG1) 103567712(GYG2)
EAI: 106821913(GYG2) 106829422(GYG1)
MYB: 102241317(GYG2) 102244006(GYG1)
MYD: 102758721(GYG2) 102770013(GYG1)
HAI: 109373337(GYG2) 109375862(GYG1)
PALE: 102881568(GYG1) 102897839(GYG2)
LAV: 100655607(GYG2) 100674831(GYG1)
MDO: 100010174(GYG2) 100012580(GYG1)
SHR: 100926747(GYG1) 100931311(GYG2)
OAA: 100075592(GYG2)
GGA: 418660(GYG2) 425050(GYG1)
MGP: 100546787(GYG2) 100549941(GYG1)
CJO: 107305955(GYG2) 107318416(GYG1)
APLA: 101789597(GYG1) 101797402(GYG2)
ACYG: 106035748(GYG2) 106047693(GYG1)
TGU: 100219874(GYG2) 100226558(GYG1)
GFR: 102031328(GYG1) 102033549(GYG2)
FAB: 101817442(GYG2) 101821265(GYG1)
PHI: 102111619(GYG2) 102114605(GYG1)
PMAJ: 107208822(GYG1) 107211785(GYG2)
CCW: 104686004(GYG2) 104688114(GYG1)
FPG: 101918206(GYG2) 101922940(GYG1)
FCH: 102045779(GYG1) 102056432(GYG2)
CLV: 102093487(GYG2) 102093817(GYG1)
EGZ: 104122295(GYG1) 104127467(GYG2)
AAM: 106484200(GYG1) 106491336(GYG2)
ASN: 102371930(GYG1)
AMJ: 102570451(GYG1)
PSS: 102455672(GYG2) 102456769(GYG1)
CMY: 102942072(GYG1) 102942316(GYG2)
CPIC: 101931228(GYG1) 101934586 101951022(GYG2)
ACS: 100557488(gyg2) 100562212(gyg1)
PVT: 110073049(GYG1) 110075590(GYG2)
PBI: 103060810(GYG1) 103068160
GJA: 107123290(GYG2) 107124665(GYG1)
XLA: 100126641(gyg2.L) 398319 446485(gyg1.S) 779133(gyg)
XTR: 394883(gyg1) 496877(gyg2)
NPR: 108785075 108797305(GYG1)
DRE: 406831(gyg1a) 415152(gyg1b) 561803(gyg2)
IPU: 100528900(glyg) 108258917(gyg2) 108278056
PRET: 103456549 103458431(gyg1) 103477311(gyg2)
SFM: 108927809 108935215(gyg1)
LCM: 102348178(GYG1) 102367058(GYG2)
CMK: 103184449(gyg1)
CIN: 100178599
SPU: 754694
APLC: 110981654
DME: Dmel_CG44244(Glycogenin)
DSI: Dsimw501_GD11576(Dsim_GD11576)
MDE: 101889378
AME: 552421
BIM: 100747793
BTER: 100651605
SOC: 105200552
AEC: 105149519
ACEP: 105622442
HST: 105188090
CFO: 105251584
LHU: 105667339
PGC: 109853746
NVI: 100122700
TCA: 656402
DPA: 109541006
NVL: 108564230
BMOR: 101741984
PRAP: 111000962
API: 100164266
TUT: 107368895
CEL: CELE_F56B6.4(gyg-1) CELE_T25E12.5(gyg-2)
CBR: CBG13253 CBG14232(Cbr-uvt-5)
BMY: Bm1_16775
TSP: Tsp_05820
CRG: 105334348
MYI: 110449160
OBI: 106872431
HMG: 100213497
CPAP: 110817512
GRA: 105792073
AIP: 107629895
LJA: Lj0g3v0036679.1(Lj0g3v0036679.1) Lj2g3v2001740.1(Lj2g3v2001740.1) Lj4g3v1273430.1(Lj4g3v1273430.1)
MCHA: 111021355
HAN: 110928800
PPP: 112286539
APRO: F751_5874
SCE: YJL137C(GLG2) YKR058W(GLG1)
ERC: Ecym_5197
KMX: KLMA_10347(GLG1)
NCS: NCAS_0B08120(NCAS0B08120)
NDI: NDAI_0B05430(NDAI0B05430)
TPF: TPHA_0A02970(TPHA0A02970)
TBL: TBLA_0D05740(TBLA0D05740)
TDL: TDEL_0H00940(TDEL0H00940)
KAF: KAFR_0G00660(KAFR0G00660)
PIC: PICST_76484(GLG2) PICST_86206(GLG1)
CAL: CAALFM_C101360CA(CaO19.3325) CAALFM_C306450WA(GLG2)
SLB: AWJ20_3597(GLG1)
NCR: NCU06698(gnn)
NTE: NEUTE1DRAFT143986(NEUTE1DRAFT_143986)
MAW: MAC_08571
CMT: CCM_03980
MBE: MBM_09939
ABE: ARB_05633
TVE: TRV_05535
PTE: PTT_07198
SMIN: v1.2.002165.t1(symbB.v1.2.002165.t1) v1.2.009238.t1(symbB.v1.2.009238.t1) v1.2.025564.t1(symbB.v1.2.025564.t1)
 » show all
Taxonomy
Reference
1  [PMID:809265]
  Authors
Krisman CR, Barengo R.
  Title
A precursor of glycogen biosynthesis: alpha-1,4-glucan-protein.
  Journal
Eur. J. Biochem. 52 (1975) 117-23.
Reference
2  [PMID:3121316]
  Authors
Pitcher J, Smythe C, Campbell DG, Cohen P.
  Title
Identification of the 38-kDa subunit of rabbit skeletal muscle glycogen synthase as glycogenin.
  Journal
Eur. J. Biochem. 169 (1987) 497-502.
Reference
3  [PMID:2970965]
  Authors
Pitcher J, Smythe C, Cohen P.
  Title
Glycogenin is the priming glucosyltransferase required for the initiation of glycogen biogenesis in rabbit skeletal muscle.
  Journal
Eur. J. Biochem. 176 (1988) 391-5.
Reference
4
  Authors
Kennedy, L.D., Kirkman, B.R., Lomako, J., Rodriguez, I.R. and Whelan, W.J.
  Title
The biogenesis of rabbit-muscle glycogen.
  Journal
In: Berman, M.C. and Opie, L.A. (Eds.), Membranes and Muscle, ICSU Press/IRL Press, Oxford, 1985, p. 65-84.
Reference
5  [PMID:4062948]
  Authors
Rodriguez IR, Whelan WJ.
  Title
A novel glycosyl-amino acid linkage: rabbit-muscle glycogen is covalently linked to a protein via tyrosine.
  Journal
Biochem. Biophys. Res. Commun. 132 (1985) 829-36.
Reference
6  [PMID:2973423]
  Authors
Lomako J, Lomako WM, Whelan WJ.
  Title
A self-glucosylating protein is the primer for rabbit muscle glycogen biosynthesis.
  Journal
FASEB. J. 2 (1988) 3097-103.
Reference
7  [PMID:7797519]
  Authors
Alonso MD, Lomako J, Lomako WM, Whelan WJ.
  Title
Catalytic activities of glycogenin additional to autocatalytic self-glucosylation.
  Journal
J. Biol. Chem. 270 (1995) 15315-9.
Reference
8  [PMID:7672505]
  Authors
Alonso MD, Lomako J, Lomako WM, Whelan WJ.
  Title
A new look at the biogenesis of glycogen.
  Journal
FASEB. J. 9 (1995) 1126-37.
Reference
9  [PMID:9857012]
  Authors
Mu J, Roach PJ.
  Title
Characterization of human glycogenin-2, a self-glucosylating initiator of liver glycogen metabolism.
  Journal
J. Biol. Chem. 273 (1998) 34850-6.
  Sequence
[hsa:8908]
Reference
10 [PMID:12051921]
  Authors
Gibbons BJ, Roach PJ, Hurley TD.
  Title
Crystal structure of the autocatalytic initiator of glycogen biosynthesis, glycogenin.
  Journal
J. Mol. Biol. 319 (2002) 463-77.
  Sequence
[ocu:100009058]
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.186
IUBMB Enzyme Nomenclature: 2.4.1.186
ExPASy - ENZYME nomenclature database: 2.4.1.186
BRENDA, the Enzyme Database: 2.4.1.186
CAS: 117590-73-5

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