KEGG   ENZYME: 2.5.1.73Help
Entry
EC 2.5.1.73                 Enzyme                                 

Name
O-phospho-L-seryl-tRNA:Cys-tRNA synthase;
SepCysS;
Sep-tRNA:Cys-tRNA synthase
Class
Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
BRITE hierarchy
Sysname
O-phospho-L-seryl-tRNACys:hydrogen sulfide 2-aminopropanoate transferase
Reaction(IUBMB)
O-phospho-L-seryl-tRNACys + sulfide = L-cysteinyl-tRNACys + phosphate [RN:R08577]
Reaction(KEGG)
Substrate
O-phospho-L-seryl-tRNACys;
sulfide [CPD:C00283]
Product
L-cysteinyl-tRNA(Cys) [CPD:C03125];
phosphate [CPD:C00009]
Comment
In organisms like Archaeoglobus fulgidus lacking EC 6.1.1.16 (cysteine---tRNA ligase) for the direct Cys-tRNACys formation, Cys-tRNACys is produced by an indirect pathway, in which EC 6.1.1.27 (O-phosphoseryl-tRNA ligase) ligates O-phosphoserine to tRNACys, and EC 2.5.1.73 converts the produced O-phospho-L-seryl-tRNACys to Cys-tRNACys. The SepRS/SepCysS pathway is the sole route for cysteine biosynthesis in the organism [1]. Methanosarcina mazei can use both pathways, the direct route using EC 6.1.1.16 (cysteine---tRNA ligase) and the indirect pathway with EC 6.1.1.27 (O-phosphoseryl-tRNA ligase) and EC 2.5.1.73 [2].
History
EC 2.5.1.73 created 2009
Pathway
Aminoacyl-tRNA biosynthesis
Orthology
K06868  
Sep-tRNA:Cys-tRNA synthetase
Genes
MJA: 
MFE: 
MVU: 
MFS: 
MIF: 
MJH: 
MIG: 
MMP: 
MMQ: 
MMX: 
MMZ: 
MMD: 
MMAK: 
MMAO: 
MAE: 
MVN: 
MVO: 
MOK: 
MAC: 
MBA: 
MBY: 
MBW: 
MBAR: 
MMA: 
MMAZ: 
MVC: 
MEK: 
MLS: 
METM: 
MEF: 
MEQ: 
MSJ: 
MSZ: 
MSW: 
MTHE: 
MTHR: 
MHOR: 
MBU: 
MMET: 
MMH: 
MEV: 
MZH: 
MPY: 
MHZ: 
MTP: 
MCJ: 
MCON_0570(pscS)
MHI: 
MHU: 
MLA: 
MEM: 
MBG: 
MEMA: 
MPI: 
MBN: 
MFO: 
MPL: 
MPD: 
MEZ: 
RCI: 
MTH: 
MMG: 
METC: 
MEL: 
MEW: 
METH: 
MFC: 
BRM9_1944(pscS)
MFI: 
MFV: 
MKA: 
AFU: 
AFG: 
APO: 
AVE: 
AST: 
FPL: 
GAC: 
GAH: 
BARC: 
BARB: 
 » show all
Taxonomy
Reference
1  [PMID:17512006]
  Authors
Fukunaga R, Yokoyama S
  Title
Structural insights into the second step of RNA-dependent cysteine biosynthesis in archaea: crystal structure of Sep-tRNA:Cys-tRNA synthase from Archaeoglobus fulgidus.
  Journal
J. Mol. Biol. 370 (2007) 128-41.
  Sequence
[afu:AF_0028]
Reference
2  [PMID:18559341]
  Authors
Hauenstein SI, Perona JJ
  Title
Redundant synthesis of cysteinyl-tRNACys in Methanosarcina mazei.
  Journal
J. Biol. Chem. 283 (2008) 22007-17.
Reference
3  [PMID:17142313]
  Authors
Yuan J, Palioura S, Salazar JC, Su D, O'Donoghue P, Hohn MJ, Cardoso AM, Whitman WB, Soll D
  Title
RNA-dependent conversion of phosphoserine forms selenocysteine in eukaryotes and  archaea.
  Journal
Proc. Natl. Acad. Sci. U. S. A. 103 (2006) 18923-7.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
1239229-21-0

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