KEGG   ENZYME: 2.5.1.87Help
Entry
EC 2.5.1.87                 Enzyme                                 

Name
ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyl diphosphate specific];
RER2;
Rer2p;
Rer2p Z-prenyltransferase;
Srt1p;
Srt2p Z-prenyltransferase;
ACPT;
dehydrodolichyl diphosphate synthase 1
Class
Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
BRITE hierarchy
Sysname
(2E,6E)-farnesyl-diphosphate:isopentenyl-diphosphate cistransferase (adding 10--55 isopentenyl units)
Reaction(IUBMB)
(2E,6E)-farnesyl diphosphate + n isopentenyl diphosphate = n diphosphate + ditrans,polycis-polyprenyl diphosphate (n = 10--55) [RN:R05556]
Reaction(KEGG)
Substrate
(2E,6E)-farnesyl diphosphate [CPD:C00448];
isopentenyl diphosphate [CPD:C00129]
Product
diphosphate [CPD:C00013];
ditrans,polycis-polyprenyl diphosphate [CPD:C05859]
Comment
The enzyme is involved in biosynthesis of dolichol (a long-chain polyprenol) with a saturated alpha-isoprene unit, which serves as a glycosyl carrier in protein glycosylation [1]. The yeast Saccharomyces cerevisiae has two different enzymes that catalyse this reaction. Rer2p synthesizes a well-defined family of polyprenols of 13--18 isoprene residues with dominating C(80) (16 isoprene residues) extending to C(120), while Srt1p synthesizes mainly polyprenol with 22 isoprene subunits. Largest Srt1p products reach C(290) [2]. The enzyme from Arabidopsis thaliana catalyses the formation of polyprenyl diphosphates with predominant carbon number C(120) [4].
History
EC 2.5.1.87 created 2010
Pathway
Terpenoid backbone biosynthesis
Biosynthesis of secondary metabolites
Orthology
K11778  
ditrans,polycis-polyprenyl diphosphate synthase
K19177  
dehydrodolichyl diphosphate syntase complex subunit NUS1
Genes
HSA: 
116150(NUS1) 79947(DHDDS)
PTR: 
456662(DHDDS) 462965(NUS1) 741304(NUS1)
PPS: 
GGO: 
101125977(DHDDS) 101137725(NUS1)
PON: 
100171948(DHDDS) 100460016(NUS1)
NLE: 
100602333(NUS1) 100603801(DHDDS)
MCC: 
714635(DHDDS) 715015(NUS1)
MCF: 
101926129(DHDDS) 102126512(NUS1)
CSAB: 
103225956(DHDDS) 103240491(NUS1)
RRO: 
104654169(NUS1) 104675752(DHDDS)
CJC: 
SBQ: 
101028238(NUS1) 101046544(DHDDS)
MMU: 
52014(Nus1) 67422(Dhdds)
RNO: 
294400(Nus1) 298541(Dhdds)
CGE: 
NGI: 
103736296(Nus1) 103737115(Dhdds)
HGL: 
101700845(Nus1) 101711794(Dhdds)
OCU: 
100339242(NUS1) 100358963(DHDDS)
TUP: 
102478223(NUS1) 102482449(DHDDS)
CFA: 
484102(NUS1) 612157(DHDDS)
AML: 
100463534(DHDDS) 100466277(NUS1)
UMR: 
103663529(NUS1) 103666770(DHDDS)
FCA: 
101085905(DHDDS) 101094180(NUS1)
PTG: 
102955679(NUS1) 102966133(DHDDS)
BTA: 
100849670(NUS1) 523264(DHDDS)
BOM: 
102270031(NUS1) 102271246(DHDDS)
PHD: 
102315363(DHDDS) 102322553(NUS1)
CHX: 
102169144(DHDDS) 102173744(NUS1)
OAS: 
101102568(NUS1) 101110654(DHDDS)
SSC: 
100154658(NUS1) 100524683(DHDDS)
CFR: 
102506402(NUS1) 102518547(DHDDS)
BACU: 
102998703(DHDDS) 103004192(NUS1)
LVE: 
103082209(NUS1) 103088197(DHDDS)
ECB: 
100057242(DHDDS) 100067138(NUS1)
MYB: 
102238802(DHDDS) 102244874(NUS1)
MYD: 
102758520(DHDDS) 102764339(NUS1)
PALE: 
102885564(NUS1) 102891420(DHDDS)
LAV: 
100666506(NUS1) 100675778(DHDDS)
MDO: 
100012356(DHDDS) 100023437(NUS1)
SHR: 
100922631(DHDDS) 100934010(NUS1)
OAA: 
100083017(DHDDS) 100088240(NUS1)
GGA: 
421733(NUS1)
MGP: 
100541233(DHDDS) 100550429(NUS1)
CJO: 
107311865(NUS1) 107324014(DHDDS)
APLA: 
101791531(NUS1) 101804205(DHDDS)
TGU: 
100187707(NUS1) 100230447(DHDDS)
GFR: 
102038851(DHDDS) 102040482(NUS1)
FAB: 
101816671(DHDDS) 101820130(NUS1)
PHI: 
102105638(DHDDS) 102108008(NUS1)
CCW: 
104691119(NUS1) 104694634(DHDDS)
FPG: 
101919832(DHDDS) 101924551(NUS1)
FCH: 
102047823(NUS1) 102049798(DHDDS)
CLV: 
102094487(NUS1) 102098335(DHDDS)
AAM: 
106485593(DHDDS) 106497998(NUS1)
ASN: 
102374803(DHDDS) 102387052(NUS1)
AMJ: 
102557872(DHDDS) 102561763(NUS1)
PSS: 
102444519(DHDDS) 102445967(NUS1)
CMY: 
102936640(NUS1) 102941423(DHDDS)
ACS: 
100552810(dhdds) 100553689(nus1)
PBI: 
103054739(NUS1) 103067643(DHDDS)
GJA: 
107108616(NUS1) 107110426(DHDDS)
XLA: 
379162 398626(dhdds.L)
XTR: 
100135207(dhdds) 548844(nus1)
DRE: 
406468(dhdds) 436629(nus1)
TRU: 
101067613(dhdds) 101071314(nus1)
TNG: 
MZE: 
101465746(dhdds) 101469329(nus1)
OLA: 
101156768(dhdds) 101169897(nus1)
XMA: 
102217942(nus1) 102232893(dhdds)
SASA: 
LCM: 
102351688(NUS1) 102361662(DHDDS)
CMK: 
103179564(dhdds) 103182587(nus1)
BFO: 
CIN: 
SPU: 
SKO: 
DME: 
DPO: 
DAN: 
DER: 
DPE: 
DSE: 
DSI: 
Dsimw501_GD16139(Dsim_GD16139) Dsimw501_GD23075(Dsim_GD23075)
DWI: 
DYA: 
Dyak_GE17374(dyak_GLEANR_1871) Dyak_GE17496(dyak_GLEANR_18820)
DGR: 
DMO: 
DVI: 
MDE: 
AGA: 
AAG: 
CQU: 
AME: 
BIM: 
BTER: 
SOC: 
AEC: 
HST: 
CFO: 
NVI: 
TCA: 
BMOR: 
DPL: 
PXY: 
API: 
PHU: 
DPX: 
ISC: 
CEL: 
CELE_T01G1.4(T01G1.4)
BMY: 
LOA: 
TSP: 
HRO: 
LGI: 
CRG: 
OBI: 
SMM: 
NVE: 
ADF: 
HMG: 
TAD: 
AQU: 
ATH: 
ALY: 
CRB: 
EUS: 
BRP: 
BNA: 
THJ: 
CIT: 
CIC: 
TCC: 
GRA: 
EGR: 
GMX: 
PVU: 
VRA: 
MTR: 
CAM: 
ADU: 
AIP: 
LJA: 
Lj0g3v0314369.1(Lj0g3v0314369.1) Lj0g3v0326269.1(Lj0g3v0326269.1) Lj0g3v0326269.2(Lj0g3v0326269.2) Lj0g3v0326269.3(Lj0g3v0326269.3) Lj0g3v0350299.1(Lj0g3v0350299.1) Lj1g3v0131170.1(Lj1g3v0131170.1) Lj1g3v0131170.2(Lj1g3v0131170.2)
FVE: 
PPER: 
PMUM: 
MDM: 
PXB: 
CSV: 
CMO: 
RCU: 
JCU: 
POP: 
POPTR_0001s25800g(POPTRDRAFT_172325) POPTR_0001s31970g(POPTRDRAFT_174968) POPTR_0002s04050g POPTR_0004s16020g(POPTRDRAFT_712930) POPTR_0005s10380g POPTR_0009s05010g(POPTRDRAFT_804506) POPTR_1031s00210g
VVI: 
SLY: 
SPEN: 
SOT: 
SIND: 
BVG: 
NNU: 
OSA: 
4324755(OJ1402_H07.30) 4328624(OJ1524_D08.29) 4331944 4340251 4343863
DOSA: 
Os01t0857200-01(Os01g0857200) Os02t0197700-01(Os02g0197700) Os03t0197000-01(Os03g0197000) Os06t0167400-01(Os06g0167400) Os07t0607700-01(Os07g0607700)
OBR: 
BDI: 
ATS: 
SBI: 
SORBI_01g013225(SORBIDRAFT_01g013225) SORBI_02g010690(SORBIDRAFT_02g010690) SORBI_02g011210(SORBIDRAFT_02g011210) SORBI_02g032900(SORBIDRAFT_02g032900) SORBI_03g040360(SORBIDRAFT_03g040360)
ZMA: 
100193048(umc2219) 100276550 100283522(GRMZM2G054803) 100284572(GRMZM2G146437) 100285459(GRMZM2G058173) 103638008(GRMZM2G093146)
SITA: 
PDA: 
EGU: 
MUS: 
ATR: 
SMO: 
PPP: 
CRE: 
VCN: 
OLU: 
OTA: 
BPG: 
MIS: 
MPP: 
CSL: 
CVR: 
APRO: 
CME: 
GSL: 
CCP: 
SCE: 
YBR002C(RER2) YDL193W(NUS1) YMR101C(SRT1)
AGO: 
ERC: 
KLA: 
LTH: 
VPO: 
ZRO: 
CGR: 
NCS: 
NCAS_0E01380(NCAS0E01380) NCAS_0E03730(NCAS0E03730) NCAS_0F01440(NCAS0F01440)
NDI: 
NDAI_0A02900(NDAI0A02900) NDAI_0G01550(NDAI0G01550) NDAI_0H02320(NDAI0H02320)
TPF: 
TPHA_0A04470(TPHA0A04470) TPHA_0G02950(TPHA0G02950) TPHA_0P00910(TPHA0P00910)
TBL: 
TBLA_0A05890(TBLA0A05890) TBLA_0B08960(TBLA0B08960) TBLA_0F00550(TBLA0F00550) TBLA_0F02120(TBLA0F02120)
TDL: 
TDEL_0A02280(TDEL0A02280) TDEL_0C02380(TDEL0C02380) TDEL_0D04190(TDEL0D04190)
KAF: 
KAFR_0A00610(KAFR0A00610) KAFR_0F02540(KAFR0F02540) KAFR_0L01300(KAFR0L01300)
PPA: 
DHA: 
PIC: 
PGU: 
SPAA: 
LEL: 
CAL: 
CTP: 
COT: 
CDU: 
CTEN: 
YLI: 
CLU: 
NCR: 
NTE: 
NEUTE1DRAFT84674(NEUTE1DRAFT_84674) NEUTE1DRAFT91460(NEUTE1DRAFT_91460)
SMP: 
PAN: 
TTT: 
MTM: 
CTHR: 
MGR: 
FGR: 
FPU: 
NHE: 
TRE: 
MAW: 
MAJ: 
CMT: 
VAL: 
VDA: 
ELA: 
SSL: 
BFU: 
MBE: 
ANI: 
AFM: 
AOR: 
AOR_1_1312024(AO090010000772) AOR_1_1932154(AO090003001085)
ANG: 
ANI_1_1896184(An04g05250) ANI_1_888034(An03g06910)
AFV: 
ACT: 
NFI: 
PCS: 
CIM: 
CPW: 
PBL: 
PBN: 
URE: 
ABE: 
TVE: 
AJE: 
PNO: 
PTE: 
BZE: 
BSC: 
BOR: 
ZTR: 
PFJ: 
BCOM: 
NPA: 
TML: 
SPO: 
CNE: 
CNB: 
CGI: 
TMS: 
PPL: 
DSQ: 
PCO: 
SHS: 
HIR: 
PSQ: 
ADL: 
GTR: 
LBC: 
MPR: 
MRR: 
CCI: 
SCM: 
ABP: 
AGABI1DRAFT110974(AGABI1DRAFT_110974) AGABI1DRAFT51492(AGABI1DRAFT_51492)
ABV: 
AGABI2DRAFT190174(AGABI2DRAFT_190174) AGABI2DRAFT197250(AGABI2DRAFT_197250)
CPUT: 
SLA: 
WSE: 
WIC: 
UMA: 
PFP: 
MGL: 
PGR: 
MLR: 
ECU: 
EIN: 
EHE: 
ERO: 
NCE: 
SRE: 
DDI: 
DPP: 
DFA: 
ACAN: 
PFA: 
MAL8P1.22(di-trans)
PCB: 
PCHAS_093380(PC000683.01.0)
PKN: 
PVX: 
PCY: 
TAN: 
TPV: 
TOT: 
BEQ: 
BBO: 
BBOV_IV002310(21.m02978)
TET: 
PTM: 
EHX: 
GTT: 
NGR: 
TVA: 
 » show all
Taxonomy
Reference
1  [PMID:11442630]
  Authors
Sato M, Fujisaki S, Sato K, Nishimura Y, Nakano A
  Title
Yeast Saccharomyces cerevisiae has two cis-prenyltransferases with different properties and localizations. Implication for their distinct physiological roles  in dolichol synthesis.
  Journal
Genes. Cells. 6 (2001) 495-506.
  Sequence
Reference
2  [PMID:17345630]
  Authors
Poznanski J, Szkopinska A
  Title
Precise bacterial polyprenol length control fails in Saccharomyces cerevisiae.
  Journal
Biopolymers. 86 (2007) 155-64.
  Sequence
Reference
3  [PMID:9858571]
  Authors
Sato M, Sato K, Nishikawa S, Hirata A, Kato J, Nakano A.
  Title
The yeast RER2 gene, identified by endoplasmic reticulum protein localization mutations, encodes cis-prenyltransferase, a key enzyme in dolichol synthesis.
  Journal
Mol. Cell. Biol. 19 (1999) 471-83.
  Sequence
Reference
4  [PMID:10764783]
  Authors
Oh SK, Han KH, Ryu SB, Kang H
  Title
Molecular cloning, expression, and functional analysis of a cis-prenyltransferase from Arabidopsis thaliana. Implications in rubber biosynthesis.
  Journal
J. Biol. Chem. 275 (2000) 18482-8.
  Sequence
[ath:AT2G23410]
Reference
5  [PMID:10908715]
  Authors
Cunillera N, Arro M, Fores O, Manzano D, Ferrer A
  Title
Characterization of dehydrodolichyl diphosphate synthase of Arabidopsis thaliana, a key enzyme in dolichol biosynthesis.
  Journal
FEBS. Lett. 477 (2000) 170-4.
  Sequence
[ath:AT2G23410]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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