KEGG   ENZYME: 2.7.1.163Help
Entry
EC 2.7.1.163                Enzyme                                 

Name
hygromycin B 4-O-kinase;
hygromycin-B kinase (ambiguous)
Class
Transferases;
Transferring phosphorus-containing groups;
Phosphotransferases with an alcohol group as acceptor
BRITE hierarchy
Sysname
ATP:hygromycin-B 4-O-phosphotransferase
Reaction(IUBMB)
ATP + hygromycin B = ADP + 4-O-phosphohygromycin B [RN:R09729]
Reaction(KEGG)
Substrate
ATP [CPD:C00002];
hygromycin B [CPD:C01925]
Product
ADP [CPD:C00008];
4-O-phosphohygromycin B [CPD:C19850]
Comment
Phosphorylates the antibiotic hygromycin B. Whereas the enzyme from Streptomyces hygroscopicus (EC 2.7.1.119; hygromycin-B 7''-O-kinase) catalyses the formation of 7''-O-phosphohygromycin B, this enzyme, found in Escherichia coli carrying a plasmid conferring resistance to hygromycin-B, forms 4-O-phosphohygromycin B.
History
EC 2.7.1.163 created 2009
Orthology
K18817  hygromycin-B 4-O-kinase
Genes
CPW: CPC735_071890
KPH: KPNIH24_26570
KPNE: KU54_01085
KPNU: LI86_28560
CJL: PJ17_01180
CCC: G157_08915
CCY: YSS_10095
PJD: Pjdr2_3115
GYM: GYMC10_4349
PBD: PBOR_25880
SALB: XNR_3282
SNA: Snas_2485
CWO: Cwoe_2613
HAU: Haur_1024
SUS: Acid_3337
 » show all
Taxonomy
Reference
1  [PMID:6318654]
  Authors
Rao RN, Allen NE, Hobbs JN Jr, Alborn WE Jr, Kirst HA, Paschal JW
  Title
Genetic and enzymatic basis of hygromycin B resistance in Escherichia coli.
  Journal
Antimicrob. Agents. Chemother. 24 (1983) 689-95.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.7.1.163
IUBMB Enzyme Nomenclature: 2.7.1.163
ExPASy - ENZYME nomenclature database: 2.7.1.163
BRENDA, the Enzyme Database: 2.7.1.163

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