KEGG ENZYME: 2.7.1.163

ENZYME: 2.7.1.163

Entry

EC 2.7.1.163                Enzyme

Name

hygromycin B 4-O-kinase;
hygromycin-B kinase (ambiguous)

Class

Transferases;
Transferring phosphorus-containing groups;
Phosphotransferases with an alcohol group as acceptor

Sysname

ATP:hygromycin-B 4-O-phosphotransferase

Reaction(IUBMB)

ATP + hygromycin B = ADP + 4-O-phosphohygromycin B [RN:R09729]

Reaction(KEGG)

R09729

Substrate

ATP [CPD:C00002];
hygromycin B [CPD:C01925]

Product

ADP [CPD:C00008];
4-O-phosphohygromycin B [CPD:C19850]

Comment

Phosphorylates the antibiotic hygromycin B. Whereas the enzyme from Streptomyces hygroscopicus (EC 2.7.1.119; hygromycin-B 7''-O-kinase) catalyses the formation of 7''-O-phosphohygromycin B, this enzyme, found in Escherichia coli carrying a plasmid conferring resistance to hygromycin-B, forms 4-O-phosphohygromycin B.

History

EC 2.7.1.163 created 2009

Orthology

K18817

hygromycin-B 4-O-kinase

Genes

CPW:	CPC735_071890

KPH:	KPNIH24_26570

KPNE:

KU54_01085

KPNU:

LI86_28560

PCOL:

F1325_16120(aph(4)-Ia)

PHYG:

JTY93_27945

MESP:

C1M53_24975

BRK:	CWS35_19325

LNE:	FZC33_33845

DEI:	C4375_01110

DSAL:

K1X15_05325

DNP:	N8A98_13345

CJL:	PJ17_01180

CCC:	G157_08915

CCY:

YSS_10095

BLEN:

NCTC4824_03381(hph)

PJD:	Pjdr2_3115

GYM:	GYMC10_4349

PBD:	PBOR_25880

POD:	PODO_15415

PAEN:

P40081_26015

PAEF:

R50345_15515

PAEH:

H70357_25420

PAEJ:

H70737_15095

PBJ:	VN24_22490

POW:	IJ21_04540

PIB:	BBD41_23800

PVO:	PVOR_23649

PALB:

EJC50_04300 EJC50_15830 EJC50_16930

PRZ:	GZH47_00160 GZH47_02360 GZH47_10095 GZH47_16675

PLYC:

GXP70_05160 GXP70_06490 GXP70_13975

PSPN:

L1F29_33455

PAUN:

MJA45_23860

PKP:	SK3146_05458(hph)

AFAS:

NZD89_24580

CCB:	Clocel_3278

CMAH:

C1I91_18425

OCW:	OW730_17970

VPY:	HZI73_09930

CCJZ:

ccrud_14530

SALB:

XNR_3282

SVIO:

HWN34_16790

HALT:

IM660_08720

RHAL:

LQF10_08195

RSUA:

LQF12_07295

MIK:	FOE78_21765

MICG:

GJV80_06970

KUT:	JJ691_41460(hph)

ATL:	Athai_11980

ASER:

Asera_61550

SNA:

Snas_2485

GLY:	K3N28_18420

CWO:

Cwoe_2613

AQZ:	KSP35_07520

HAU:

Haur_1024

KBS:	EPA93_03520

PMET:

G4Y79_04240

BUY:	D8S85_06040

BFH:	F1611_17345

COY:	HF329_12670

PGO:	FSB84_21180

» show all

Reference

1 [PMID:6318654]

Authors

Rao RN, Allen NE, Hobbs JN Jr, Alborn WE Jr, Kirst HA, Paschal JW

Title

Genetic and enzymatic basis of hygromycin B resistance in Escherichia coli.

Journal

Antimicrob Agents Chemother 24:689-95 (1983)
DOI:10.1128/AAC.24.5.689

Sequence

[ag:CAA24743]

Other DBs

ExplorEnz - The Enzyme Database:

2.7.1.163

IUBMB Enzyme Nomenclature:

2.7.1.163

ExPASy - ENZYME nomenclature database:

2.7.1.163

BRENDA, the Enzyme Database:

2.7.1.163

DBGET integrated database retrieval system