KEGG   ENZYME: 2.7.1.163Help
Entry
EC 2.7.1.163                Enzyme                                 

Name
hygromycin B 4-O-kinase;
hygromycin-B kinase (ambiguous)
Class
Transferases;
Transferring phosphorus-containing groups;
Phosphotransferases with an alcohol group as acceptor
BRITE hierarchy
Sysname
ATP:hygromycin-B 4-O-phosphotransferase
Reaction(IUBMB)
ATP + hygromycin B = ADP + 4-O-phosphohygromycin B [RN:R09729]
Reaction(KEGG)
Substrate
ATP [CPD:C00002];
hygromycin B [CPD:C01925]
Product
ADP [CPD:C00008];
4-O-phosphohygromycin B [CPD:C19850]
Comment
Phosphorylates the antibiotic hygromycin B. Whereas the enzyme from Streptomyces hygroscopicus (EC 2.7.1.119; hygromycin-B 7''-O-kinase) catalyses the formation of 7''-O-phosphohygromycin B, this enzyme, found in Escherichia coli carrying a plasmid conferring resistance to hygromycin-B, forms 4-O-phosphohygromycin B.
History
EC 2.7.1.163 created 2009
Reference
1  [PMID:6318654]
  Authors
Rao RN, Allen NE, Hobbs JN Jr, Alborn WE Jr, Kirst HA, Paschal JW
  Title
Genetic and enzymatic basis of hygromycin B resistance in Escherichia coli.
  Journal
Antimicrob. Agents. Chemother. 24 (1983) 689-95.
  Organism
Escherichia coli
  Sequence
[up:P00557]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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