KEGG   ENZYME: 2.8.2.23Help
Entry
EC 2.8.2.23                 Enzyme                                 

Name
[heparan sulfate]-glucosamine 3-sulfotransferase 1;
heparin-glucosamine 3-O-sulfotransferase;
3'-phosphoadenylyl-sulfate:heparin-glucosamine 3-O-sulfotransferase;
glucosaminyl 3-O-sulfotransferase;
heparan sulfate D-glucosaminyl 3-O-sulfotransferase;
isoform/isozyme 1 (3-OST-1, HS3ST1)
Class
Transferases;
Transferring sulfur-containing groups;
Sulfotransferases
BRITE hierarchy
Sysname
3'-phosphoadenylyl-sulfate:[heparan sulfate]-glucosamine 3-sulfotransferase
Reaction(IUBMB)
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine = adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 3-sulfate [RN:R05798]
Reaction(KEGG)
Substrate
3'-phosphoadenylyl sulfate [CPD:C00053];
[heparan sulfate]-glucosamine [CPD:C17141]
Product
adenosine 3',5'-bisphosphate [CPD:C00054];
[heparan sulfate]-glucosamine 3-sulfate [CPD:C11553]
Comment
This enzyme differs from the other [heparan sulfate]-glucosamine 3-sulfotransferases [EC 2.8.2.29 ([heparan sulfate]-glucosamine 3-sulfotransferase 2) and EC 2.8.2.30 ([heparan sulfate]-glucosamine 3-sulfotransferase 3)] by being the most selective for a precursor of the antithrombin-binding site. It has a minimal acceptor sequence of: -> GlcNAc6S-> GlcA-> GlcN2S*+/-6S-> IdoA2S-> GlcN2S-> , the asterisk marking the target (symbols as in {iupac/2carb/38::2-Carb-38}) using +/- to mean the presence or absence of a substituent, and > to separate a predominant structure from a minor one. Thus Glc(N2S > NAc) means a residue of glucosamine where the N carries a sulfo group mainly but occasionally an acetyl group.) [1-4]. It can also modify other precursor sequences within heparan sulfate but this action does not create functional antithrombin-binding sites. These precursors are variants of the consensus sequence: -> Glc(N2S > NAc)+/-6S-> GlcA-> GlcN2S*+/-6S-> GlcA > IdoA+/-2S-> Glc(N2S/NAc)+/-6S-> [5]. If the heparan sulfate substrate lacks 2-O-sulfation of GlcA residues, then enzyme specificity is expanded to modify selected glucosamine residues preceded by IdoA as well as GlcA [6].
History
EC 2.8.2.23 created 1992, modified 2001
Pathway
Glycosaminoglycan biosynthesis - heparan sulfate / heparin
Orthology
K01024  
[heparan sulfate]-glucosamine 3-sulfotransferase 1
K08104  
[heparan sulfate]-glucosamine 3-sulfotransferase 5
Genes
HSA: 
222537(HS3ST5) 9957(HS3ST1)
PTR: 
471144(HS3ST1) 744298(HS3ST5)
PPS: 
100967734(HS3ST1) 100979280(HS3ST5)
GGO: 
101134668(HS3ST1) 101154208(HS3ST5)
PON: 
100445561(HS3ST5) 100449950(HS3ST1)
NLE: 
100592585(HS3ST5) 100601459(HS3ST1)
MCC: 
693684(HS3ST5) 713374(HS3ST1)
MCF: 
101865986(HS3ST1) 102141292(HS3ST5)
RRO: 
104657111(HS3ST5) 104657730(HS3ST1)
CJC: 
100392281(HS3ST1) 100402689(HS3ST5)
MMU: 
15476(Hs3st1) 319415(Hs3st5)
RNO: 
294449(Hs3st5) 84406(Hs3st1)
CGE: 
100759378(Hs3st1) 100763965(Hs3st5)
NGI: 
103728889(Hs3st1) 103733777(Hs3st5)
HGL: 
101698728(Hs3st1) 101710328(Hs3st5)
OCU: 
100343879(HS3ST1) 100346980(HS3ST5)
TUP: 
102468225(HS3ST5) 102481838(HS3ST1)
CFA: 
403672(HS3ST1) 481968(HS3ST5)
AML: 
100478675(HS3ST5) 105241055(HS3ST1)
UMR: 
103658356(HS3ST1) 103676972(HS3ST5)
FCA: 
101088412(HS3ST5) 101090103(HS3ST1)
PTG: 
102952040(HS3ST5) 102965928(HS3ST1)
BTA: 
538691(HS3ST1) 540355(HS3ST5)
BOM: 
102279879(HS3ST5) 102279923(HS3ST1)
PHD: 
102325819(HS3ST5) 102327107(HS3ST1)
CHX: 
102169088(HS3ST1) 102176690(HS3ST5)
OAS: 
101104909(HS3ST1) 101107448(HS3ST5)
SSC: 
100155403(HS3ST5) 100628223(HS3ST1)
CFR: 
102503844(HS3ST5) 102515681(HS3ST1)
BACU: 
103015413(HS3ST1) 103016478(HS3ST5)
LVE: 
103068414(HS3ST5) 103089700(HS3ST1)
ECB: 
100056091(HS3ST1) 100066938(HS3ST5)
MYB: 
102239991(HS3ST5) 102241744(HS3ST1)
MYD: 
102756820(HS3ST1) 102773229(HS3ST5)
PALE: 
102879059(HS3ST5) 102886681(HS3ST1)
MDO: 
100012960(HS3ST1) 100022809(HS3ST5)
SHR: 
100917710(HS3ST1) 100926311(HS3ST5)
OAA: 
100079950(HS3ST5) 100087772(HS3ST1)
GGA: 
422840(HS3ST1) 423822 428621(HS3ST5)
MGP: 
100541206 100541289(HS3ST5) 100546598(HS3ST1)
CJO: 
107311893(HS3ST5) 107314022(HS3ST1) 107315944
APLA: 
101791819(HS3ST5) 101793237(HS3ST1) 101804190
TGU: 
GFR: 
102031527(HS3ST1) 102038656(HS3ST5) 102043838
FAB: 
101809457(HS3ST5) 101814866(HS3ST1) 101816899
PHI: 
102102605(HS3ST1) 102104930(HS3ST5) 102109527
CCW: 
104690727(HS3ST1) 104691254(HS3ST5) 104696091
FPG: 
101919371 101923794(HS3ST5) 101923963(HS3ST1)
FCH: 
102053103(HS3ST1) 102054204 102057031(HS3ST5)
CLV: 
102091303(HS3ST1) 102094670 102096271(HS3ST5)
AAM: 
106489636(HS3ST5) 106494700 106498671(HS3ST1)
ASN: 
102376753(HS3ST1) 102378310(HS3ST5) 102385617
AMJ: 
102560943(HS3ST5) 102573417(HS3ST1L1) 102574400(HS3ST1)
PSS: 
CMY: 
ACS: 
PBI: 
GJA: 
XLA: 
398971(hs3st1)
XTR: 
100125092 100145790(hs3st5) 496940(hs3st1)
DRE: 
557111(hs3st1l1) 565201(hs3st1)
TRU: 
MZE: 
OLA: 
XMA: 
LCM: 
CMK: 
CIN: 
SPU: 
SKO: 
DME: 
Dmel_CG33147(Hs3st-A)
DPO: 
DAN: 
DER: 
DPE: 
DSE: 
DSI: 
Dsimw501_GD11407(Dsim_GD11407)
DWI: 
DYA: 
Dyak_GE11996(dyak_GLEANR_12286)
DGR: 
DMO: 
DVI: 
MDE: 
AGA: 
AAG: 
CQU: 
AME: 
412956(Hs3st-A)
SOC: 
AEC: 
HST: 
CFO: 
NVI: 
TCA: 
BMOR: 
PXY: 
API: 
PHU: 
ISC: 
BMY: 
LOA: 
TSP: 
HRO: 
LGI: 
CRG: 
OBI: 
AQU: 
GTT: 
 » show all
Taxonomy
Reference
1  [PMID:3139669]
  Authors
Kusche M, Backstrom G, Riesenfeld J, Petitou M, Choay J, Lindahl U.
  Title
Biosynthesis of heparin. O-sulfation of the antithrombin-binding region.
  Journal
J. Biol. Chem. 263 (1988) 15474-84.
Reference
2  [PMID:8900197]
  Authors
Shworak NW, Fritze LM, Liu J, Butler LD, Rosenberg RD.
  Title
Cell-free synthesis of anticoagulant heparan sulfate reveals a limiting converting activity that modifies an excess precursor pool.
  Journal
J. Biol. Chem. 271 (1996) 27063-71.
Reference
3  [PMID:8900198]
  Authors
Liu J, Shworak NW, Fritze LM, Edelberg JM, Rosenberg RD.
  Title
Purification of heparan sulfate D-glucosaminyl 3-O-sulfotransferase.
  Journal
J. Biol. Chem. 271 (1996) 27072-82.
  Sequence
[hsa:9957]
Reference
4  [PMID:9346953]
  Authors
Shworak NW, Liu J, Fritze LM, Schwartz JJ, Zhang L, Logeart D, Rosenberg RD.
  Title
Molecular cloning and expression of mouse and human cDNAs encoding heparan sulfate D-glucosaminyl 3-O-sulfotransferase.
  Journal
J. Biol. Chem. 272 (1997) 28008-19.
  Sequence
[hsa:9957] [mmu:15476]
Reference
5  [PMID:10026187]
  Authors
Zhang L, Yoshida K, Liu J, Rosenberg RD.
  Title
Anticoagulant heparan sulfate precursor structures in F9 embryonal carcinoma cells.
  Journal
J. Biol. Chem. 274 (1999) 5681-91.
Reference
6  [PMID:11375390]
  Authors
Zhang L, Lawrence R, Schwartz JJ, Bai X, Wei G, Esko JD, Rosenberg RD.
  Title
The effect of precursor structures on the action of glucosaminyl 3-O-sulfotransferase-1 and the biosynthesis of anticoagulant heparan sulfate.
  Journal
J. Biol. Chem. 276 (2001) 28806-13.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
183257-54-7

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