KEGG   ENZYME: 3.11.1.3Help
Entry
EC 3.11.1.3                 Enzyme                                 

Name
phosphonopyruvate hydrolase;
PPH
Class
Hydrolases;
Acting on carbon-phosphorus bonds;
Acting on carbon-phosphorus bonds (only sub-subclass identified to date)
BRITE hierarchy
Reaction(IUBMB)
3-phosphonopyruvate + H2O = pyruvate + phosphate [RN:R08200]
Reaction(KEGG)
Substrate
3-phosphonopyruvate [CPD:C02798];
H2O [CPD:C00001]
Product
pyruvate [CPD:C00022];
phosphate [CPD:C00009]
Comment
Highly specific for phosphonopyruvate as substrate [2]. The reaction is not inhibited by phosphate but is inhibited by the phosphonates phosphonoformic acid, hydroxymethylphosphonic acid and 3-phosphonopropanoic acid [2]. The enzyme is activated by the divalent cations Co2+, Mg2+ and Mn2+. This enzyme is a member of the phosphoenolpyruvate mutase/isocitrate lyase superfamily [3].
History
EC 3.11.1.3 created 2007
Pathway
Phosphonate and phosphinate metabolism
Orthology
K19669  
phosphonopyruvate hydrolase
Genes
VPD: 
Taxonomy
Reference
1  [PMID:10648102]
  Authors
Ternan NG, Hamilton JT, Quinn JP.
  Title
Initial in vitro characterisation of phosphonopyruvate hydrolase, a novel phosphate starvation-independent, carbon-phosphorus bond cleavage enzyme in Burkholderia cepacia Pal6.
  Journal
Arch. Microbiol. 173 (2000) 35-41.
Reference
2  [PMID:12697754]
  Authors
Kulakova AN, Wisdom GB, Kulakov LA, Quinn JP.
  Title
The purification and characterization of phosphonopyruvate hydrolase, a novel carbon-phosphorus bond cleavage enzyme from Variovorax sp Pal2.
  Journal
J. Biol. Chem. 278 (2003) 23426-31.
  Sequence
Reference
3  [PMID:16981709]
  Authors
Chen CC, Han Y, Niu W, Kulakova AN, Howard A, Quinn JP, Dunaway-Mariano D, Herzberg O.
  Title
Structure and kinetics of phosphonopyruvate hydrolase from Variovorax sp. Pal2: new insight into the divergence of catalysis within the PEP mutase/isocitrate lyase superfamily.
  Journal
Biochemistry. 45 (2006) 11491-504.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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