| Entry |
|
|---|
| Name |
phosphonopyruvate hydrolase;
PPH
|
|---|
| Class |
Hydrolases;
Acting on carbon-phosphorus bonds;
Acting on carbon-phosphorus bonds (only sub-subclass identified to date)
 |
|---|
| Reaction(IUBMB) |
3-phosphonopyruvate + H2O = pyruvate + phosphate [RN: R08200]
|
|---|
| Reaction(KEGG) |
|
|---|
| Substrate |
|
|---|
| Product |
|
|---|
| Comment |
Highly specific for phosphonopyruvate as substrate [2]. The reaction is not inhibited by phosphate but is inhibited by the phosphonates phosphonoformic acid, hydroxymethylphosphonic acid and 3-phosphonopropanoic acid [2]. The enzyme is activated by the divalent cations Co2+, Mg2+ and Mn2+. This enzyme is a member of the phosphoenolpyruvate mutase/isocitrate lyase superfamily [3].
|
|---|
| Pathway |
| Phosphonate and phosphinate metabolism |
|
|---|
| Reference |
|
|---|
| Authors |
Ternan NG, Hamilton JT, Quinn JP. |
|---|
| Title |
Initial in vitro characterisation of phosphonopyruvate hydrolase, a novel phosphate starvation-independent, carbon-phosphorus bond cleavage enzyme in Burkholderia cepacia Pal6. |
|---|
| Journal |
Arch. Microbiol. 173 (2000) 35-41. |
|---|
| Organism |
Burkholderia cepacia [GN: bam] |
|---|
| Reference |
|
|---|
| Authors |
Kulakova AN, Wisdom GB, Kulakov LA, Quinn JP. |
|---|
| Title |
The purification and characterization of phosphonopyruvate hydrolase, a novel carbon-phosphorus bond cleavage enzyme from Variovorax sp Pal2. |
|---|
| Journal |
J. Biol. Chem. 278 (2003) 23426-31. |
|---|
| Reference |
|
|---|
| Authors |
Chen CC, Han Y, Niu W, Kulakova AN, Howard A, Quinn JP, Dunaway-Mariano D, Herzberg O. |
|---|
| Title |
Structure and kinetics of phosphonopyruvate hydrolase from Variovorax sp. Pal2: new insight into the divergence of catalysis within the PEP mutase/isocitrate lyase superfamily. |
|---|
| Journal |
Biochemistry. 45 (2006) 11491-504. |
|---|
| Other DBs |
ExplorEnz - The Enzyme Database: IUBMB Enzyme Nomenclature: ExPASy - ENZYME nomenclature database: BRENDA, the Enzyme Database: |
|---|
