KEGG   ENZYME: 3.2.1.153Help
Entry
EC 3.2.1.153                Enzyme                                 

Name
fructan beta-(2,1)-fructosidase;
beta-(2-1)-D-fructan fructohydrolase;
beta-(2-1)fructan exohydrolase;
inulinase;
1-FEH II;
1-fructan exohydrolase;
1-FEH w1;
1-FEH w2;
beta-(2-1)-linkage-specific fructan-beta-fructosidase;
beta-(2,1)-D-fructan fructohydrolase
Class
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
BRITE hierarchy
Sysname
beta-(2->1)-D-fructan fructohydrolase
Reaction(IUBMB)
Hydrolysis of terminal, non-reducing (2->1)-linked beta-D-fructofuranose residues in fructans
Comment
Possesses one of the activities of EC 3.2.1.80, fructan beta-fructosidase. While the best substrates are the inulin-type fructans, such as 1-kestose [beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl alpha-D-glucopyranoside] and 1,1-nystose [beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl alpha-D-glucopyranoside], some (but not all) levan-type fructans can also be hydrolysed, but more slowly [see EC 3.2.1.154, fructan beta-(2,6)-fructosidase]. Sucrose, while being a very poor substrate, can substantially inhibit enzyme activity in some cases.
History
EC 3.2.1.153 created 2005
Reference
1
  Authors
De Roover, J., Van Laere, A., De Winter, M., Timmermans, J.W. and Van den Ende, W.
  Title
Purification and properties of a second fructan exohydrolase from the roots of Cichorium intybus.
  Journal
Physiol. Plant. 106 (1999) 28-34.
Reference
2  [PMID:12586886]
  Authors
Van Den Ende W, Clerens S, Vergauwen R, Van Riet L, Van Laere A, Yoshida M, Kawakami A.
  Title
Fructan 1-exohydrolases. beta-(2,1)-trimmers during graminan biosynthesis in stems of wheat? Purification, characterization, mass mapping, and cloning of two fructan 1-exohydrolase isoforms.
  Journal
Plant. Physiol. 131 (2003) 621-31.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
1000593-08-7

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