KEGG   ENZYME: 3.2.1.161Help
Entry
EC 3.2.1.161                Enzyme                                 

Name
beta-apiosyl-beta-glucosidase;
isoflavonoid-7-O-beta[D-apiosyl-(1->6)-beta-D-glucoside] disaccharidase;
isoflavonoid 7-O-beta-apiosyl-glucoside beta-glucosidase;
furcatin hydrolase
Class
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
BRITE hierarchy
Sysname
7-[beta-D-apiofuranosyl-(1->6)-beta-D-glucopyranosyloxy]isoflavonoid beta-D-apiofuranosyl-(1->6)-D-glucohydrolase
Reaction(IUBMB)
7-[beta-D-apiofuranosyl-(1->6)-beta-D-glucopyranosyloxy]isoflavonoid + H2O = a 7-hydroxyisoflavonoid + beta-D-apiofuranosyl-(1->6)-D-glucose
Substrate
7-[beta-D-apiofuranosyl-(1->6)-beta-D-glucopyranosyloxy]isoflavonoid;
H2O [CPD:C00001]
Product
7-hydroxyisoflavonoid;
beta-D-apiofuranosyl-(1->6)-D-glucose
Comment
The enzyme from the tropical tree Dalbergia nigrescens Kurz belongs in glycosyl hydrolase family 1. The enzyme removes disaccharides from the natural substrates dalpatein 7-O-beta-D-apiofuranosyl-(1->6)-beta-D-glucopyranoside and 7-hydroxy-2',4',5',6-tetramethoxy-7-O-beta-D-apiofuranosyl-(1->6)-beta-D-glucopyranoside (dalnigrein 7-O-beta-D-apiofuranosyl-(1->6)-beta-D-glucopyranoside) although it can also remove a single glucose residue from isoflavonoid 7-O-glucosides [2]. Daidzin and genistin are also substrates.
History
EC 3.2.1.161 created 2006
Reference
1  [PMID:240725]
  Authors
Hosel W, Barz W.
  Title
Beta-Glucosidases from Cicer arietinum L. Purification and Properties of isoflavone-7-O-glucoside-specific beta-glucosidases.
  Journal
Eur. J. Biochem. 57 (1975) 607-16.
Reference
2  [PMID:16098548]
  Authors
Chuankhayan P, Hua Y, Svasti J, Sakdarat S, Sullivan PA, Ketudat Cairns JR.
  Title
Purification of an isoflavonoid 7-O-beta-apiosyl-glucoside beta-glycosidase and its substrates from Dalbergia nigrescens Kurz.
  Journal
Phytochemistry. 66 (2005) 1880-9.
Reference
3  [PMID:14976214]
  Authors
Ahn YO, Mizutani M, Saino H, Sakata K.
  Title
Furcatin hydrolase from Viburnum furcatum Blume is a novel disaccharide-specific acuminosidase in glycosyl hydrolase family 1.
  Journal
J. Biol. Chem. 279 (2004) 23405-14.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
1000598-83-3

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