beta-agarase;
agarase (ambiguous);
AgaA;
AgaB;
endo-beta-agarase;
agarose 3-glycanohydrolase (incorrect)

Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

agarose 4-glycanohydrolase

Hydrolysis of (1->4)-beta-D-galactosidic linkages in agarose, giving the tetramer as the predominant product

Also acts on porphyran, but more slowly [1]. This enzyme cleaves the beta-(1->4) linkages of agarose in a random manner with retention of the anomeric-bond configuration, producing beta-anomers that give rise progressively to alpha-anomers when mutarotation takes place [6]. The end products of hydrolysis are neoagarotetraose and neoagarohexaose in the case of AgaA from the marine bacterium Zobellia galactanivorans, and neoagarotetraose and neoagarobiose in the case of AgaB [6].

1  [PMID:5386190]

Duckworth M, Turvey JR.

The action of a bacterial agarase on agarose, porphyran and alkali-treated porphyran.

Biochem. J. 113 (1969) 687-92.

Cytophaga sp.

2  [PMID:12970344]

Allouch J, Jam M, Helbert W, Barbeyron T, Kloareg B, Henrissat B, Czjzek M.

The three-dimensional structures of two beta-agarases.

J. Biol. Chem. 278 (2003) 47171-80.

Zobellia galactanivorans

3  [PMID:15170112]

Ohta Y, Nogi Y, Miyazaki M, Li Z, Hatada Y, Ito S, Horikoshi K.

Enzymatic properties and nucleotide and amino acid sequences of a thermostable beta-agarase from the novel marine isolate, JAMB-A94.

Biosci. Biotechnol. Biochem. 68 (2004) 1073-81.

Microbulbifer salipaludis

4  [PMID:15088129]

Ohta Y, Hatada Y, Nogi Y, Miyazaki M, Li Z, Akita M, Hidaka Y, Goda S, Ito S, Horikoshi K.

Enzymatic properties and nucleotide and amino acid sequences of a thermostable beta-agarase from a novel species of deep-sea Microbulbifer.

Appl. Microbiol. Biotechnol. 64 (2004) 505-14.

Microbulbifer elongataus, Microbulbifer salipaludis, Microbulbifer elongataus, Microbulbifer hydrolyticus

5  [PMID:8517750]

Sugano Y, Terada I, Arita M, Noma M, Matsumoto T.

Purification and characterization of a new agarase from a marine bacterium, Vibrio sp. strain JT0107.

Appl. Environ. Microbiol. 59 (1993) 1549-54.

Undaria pinnatiJida, Laminaria sp., Vibrio sp.

6  [PMID:15456406]

Jam M, Flament D, Allouch J, Potin P, Thion L, Kloareg B, Czjzek M, Helbert W, Michel G, Barbeyron T.

The endo-beta-agarases AgaA and AgaB from the marine bacterium Zobellia galactanivorans: two paralogue enzymes with different molecular organizations and catalytic behaviours.

Biochem. J. 385 (2005) 703-13.

Zobellia galactanivorans