KEGG   ENZYME: 3.2.1.97Help
Entry
EC 3.2.1.97                 Enzyme                                 

Name
endo-alpha-N-acetylgalactosaminidase;
endo-alpha-acetylgalactosaminidase;
endo-alpha-N-acetyl-D-galactosaminidase;
mucinaminylserine mucinaminidase;
D-galactosyl-3-(N-acetyl-alpha-D-galactosaminyl)-L-serine mucinaminohydrolase;
endo-alpha-GalNAc-ase;
glycopeptide alpha-N-acetylgalactosaminidase;
D-galactosyl-N-acetyl-alpha-D-galactosamine D-galactosyl-N-acetyl-galactosaminohydrolase
Class
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
BRITE hierarchy
Sysname
glycopeptide-D-galactosyl-N-acetyl-alpha-D-galactosamine D-galactosyl-N-acetyl-galactosaminohydrolase
Reaction(IUBMB)
beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-[glycoprotein]-L-serine/L-threonine + H2O = beta-D-galactosyl-(1->3)-N-acetyl-D-galactosamine + [glycoprotein]-L-serine/L-threonine [RN:R04527 R06140]
Reaction(KEGG)
Substrate
beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-[glycoprotein]-L-serine/L-threonine;
H2O [CPD:C00001]
Product
beta-D-galactosyl-(1->3)-N-acetyl-D-galactosamine [CPD:C07278];
[glycoprotein]-L-serine/L-threonine
Comment
The enzyme catalyses the liberation of Gal-(1->3)-beta-GalNAc alpha-linked to serine or threonine residues of mucin-type glycoproteins. EngBF from the bacterium Bifidobacterium longum specifically acts on core 1-type O-glycan to release the disaccharide Gal-(1->3)-beta-GalNAc. The enzymes from the bacteria Clostridium perfringens, Enterococcus faecalis, Propionibacterium acnes and Alcaligenes faecalis show broader specificity (e.g. they can also release the core 2 trisaccharide Gal-(1->3)-beta-(GlcNAc-(1->6)-beta)-GalNAc or the core 3 disaccharide GlcNAc-(1->3)-beta-GalNAc) [1,2]. The enzyme may play an important role in the degradation and utilization of mucins having core 1 O-glycan.
History
EC 3.2.1.97 created 1978 (EC 3.2.1.110 created 1984, incorporated 2008), modified 2008, modified 2011
Orthology
K17624  endo-alpha-N-acetylgalactosaminidase
Genes
BCR: BCAH187_A2112 BCAH187_A2113
BCQ: BCQ_1996(gcaD)
BNC: BCN_1925 BCN_1926
BTF: YBT020_10440 YBT020_10445
VHL: BME96_15600
VIG: BKP57_16755
VIL: CFK37_08520
VPN: A21D_00287
SSD: SPSINT_2361
SDT: SPSE_0100(spsG)
SSCH: LH95_00870
SSCZ: RN70_01085
SPN: SP_0368
SPD: SPD_0335
SPR: spr0328
SPW: SPCG_0364
SPX: SPG_0333
SPV: SPH_0475
SJJ: SPJ_0354
SPP: SPP_0406
SNT: SPT_0411
SNP: SPAP_0392
SND: MYY_0445
SPNN: T308_01820
SMB: smi_1538
SOR: SOR_1643
EFA: EF1800
EFL: EF62_2171
EFS: EFS1_1608
EFQ: DR75_793
MPX: MPD5_0190
CPE: CPE0693
CPF: CPF_0685
APR: Apre_1674
SCO: SCO6348(SC3A7.16c)
SGB: WQO_25750
SCT: SCAT_1146
SVE: SVEN_5220
SALS: SLNWT_5875
SFI: SFUL_5426
SALU: DC74_2489
SALL: SAZ_13790
STRE: GZL_06509
SLD: T261_6164
SPRI: SPRI_2326
STRD: NI25_36400
SALF: SMD44_02207(engBF)
SALJ: SMD11_5036(engBF)
LMOI: VV02_02330
KAL: KALB_2141
TPYO: X956_07740
BLO: BL0464
BLJ: BLD_1258(aprE)
BLF: BLIF_0157
BLB: BBMN68_1202(aprE)
BLM: BLLJ_0168
BBI: BBIF_0285
BBP: BBPR_0264
BBF: BBB_0248
 » show all
Taxonomy
Reference
1  [PMID:18559962]
  Authors
Ashida H, Maki R, Ozawa H, Tani Y, Kiyohara M, Fujita M, Imamura A, Ishida H, Kiso M, Yamamoto K
  Title
Characterization of two different endo-alpha-N-acetylgalactosaminidases from probiotic and pathogenic enterobacteria, Bifidobacterium longum and Clostridium perfringens.
  Journal
Glycobiology. 18 (2008) 727-34.
  Sequence
[cpe:CPE0693]
Reference
2  [PMID:18635885]
  Authors
Koutsioulis D, Landry D, Guthrie EP
  Title
Novel endo-alpha-N-acetylgalactosaminidases with broader substrate specificity.
  Journal
Glycobiology. 18 (2008) 799-805.
Reference
3  [PMID:16141207]
  Authors
Fujita K, Oura F, Nagamine N, Katayama T, Hiratake J, Sakata K, Kumagai H, Yamamoto K
  Title
Identification and molecular cloning of a novel glycoside hydrolase family of core 1 type O-glycan-specific endo-alpha-N-acetylgalactosaminidase from Bifidobacterium longum.
  Journal
J. Biol. Chem. 280 (2005) 37415-22.
  Sequence
[blm:BLLJ_0168]
Reference
4  [PMID:19502354]
  Authors
Suzuki R, Katayama T, Kitaoka M, Kumagai H, Wakagi T, Shoun H, Ashida H, Yamamoto K, Fushinobu S
  Title
Crystallographic and mutational analyses of substrate recognition of endo-alpha-N-acetylgalactosaminidase from Bifidobacterium longum.
  Journal
J. Biochem. 146 (2009) 389-98.
Reference
5  [PMID:19194003]
  Authors
Gregg KJ, Boraston AB
  Title
Cloning, recombinant production, crystallization and preliminary X-ray diffraction analysis of a family 101 glycoside hydrolase from Streptococcus pneumoniae.
  Journal
Acta. Crystallogr. Sect. F. Struct. Biol. Cryst. Commun. 65 (2009) 133-5.
Reference
6  [PMID:10620364]
  Authors
Ashida H, Yamamoto K, Murata T, Usui T, Kumagai H
  Title
Characterization of endo-alpha-N-acetylgalactosaminidase from Bacillus sp. and syntheses of neo-oligosaccharides using its transglycosylation activity.
  Journal
Arch. Biochem. Biophys. 373 (2000) 394-400.
Reference
7  [PMID:18725192]
  Authors
Goda HM, Ushigusa K, Ito H, Okino N, Narimatsu H, Ito M
  Title
Molecular cloning, expression, and characterization of a novel endo-alpha-N-acetylgalactosaminidase from Enterococcus faecalis.
  Journal
Biochem. Biophys. Res. Commun. 375 (2008) 441-6.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 3.2.1.97
IUBMB Enzyme Nomenclature: 3.2.1.97
ExPASy - ENZYME nomenclature database: 3.2.1.97
BRENDA, the Enzyme Database: 3.2.1.97
CAS: 59793-96-3

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