KEGG   ENZYME: 3.4.23.18Help
Entry
EC 3.4.23.18                Enzyme                                 

Name
aspergillopepsin I;
Aspergillus acid protease;
Aspergillus acid proteinase;
Aspergillus aspartic proteinase;
Aspergillus awamori acid proteinase;
Aspergillus carboxyl proteinase;
(see also Comments);
carboxyl proteinase;
Aspergillus kawachii aspartic proteinase;
Aspergillus saitoi acid proteinase;
pepsin-type aspartic proteinase;
Aspergillus niger acid proteinase;
sumizyme AP;
proctase P;
denapsin;
denapsin XP 271;
proctase
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Aspartic endopeptidases
BRITE hierarchy
Reaction(IUBMB)
Hydrolysis of proteins with broad specificity. Generally favours hydrophobic residues in P1 and P1', but also accepts Lys in P1, which leads to activation of trypsinogen. Does not clot milk
Comment
Found in a variety of Aspergillus species (imperfect fungi): Aspergillus awamori (awamorin, aspergillopepsin A: [8]), A. foetidus (aspergillopepsin F: [6]), A. fumigatus [7], A. kawachii [9], A. niger (proteinase B, proctase B: [2,4]), A. oryzae (trypsinogen kinase: [3,10]), A. saitoi (aspergillopeptidase A: [10]), and A. sojae [5,10]. In peptidase family A1 (pepsin A family). Formerly included in EC 3.4.23.6
History
EC 3.4.23.18 created 1992 (EC 3.4.23.6 created 1961 as EC 3.4.4.17, transferred 1972 to EC 3.4.23.6, modified 1981 [EC 3.4.23.7, EC 3.4.23.8, EC 3.4.23.9, EC 3.4.23.10, EC 3.4.99.1, EC 3.4.99.15 and EC 3.4.99.25 all created 1972 and incorporated 1978], part incorporated 1992)
Orthology
K06004  
aspergillopepsin I
Genes
NCR: 
PAN: 
MGR: 
NHE: 
MAW: 
MAJ: 
SSL: 
BFU: 
ANI: 
AFM: 
AOR: 
AOR_1_830094(AO090120000474)
ANG: 
ANI_1_654124(An14g04710)
AFV: 
ACT: 
NFI: 
PCS: 
URE: 
ABE: 
TVE: 
PNO: 
BZE: 
BSC: 
BOR: 
 » show all
Taxonomy
Reference
1  [PMID:4565799]
  Authors
Kovaleva GG, Shimanskaya MP, Stepanov VM.
  Title
The site of diazoacetyl inhibitor attachment to acid proteinase of Aspergillus awamori--an analog of penicillopepsin and pepsin.
  Journal
Biochem. Biophys. Res. Commun. 49 (1972) 1075-81.
Reference
2  [PMID:4593189]
  Authors
Morihara K, Oka T.
  Title
Comparative specificity of microbial acid proteinases for synthetic peptides. 3. Relationship with their trypsinogen activating ability.
  Journal
Arch. Biochem. Biophys. 157 (1973) 561-72.
Reference
3  [PMID:239702]
  Authors
Davidson R, Gertler A, Hofmann T.
  Title
Aspergillus oryzae acid proteinase. Purification and properties, and formation of pi-chymotrypsin.
  Journal
Biochem. J. 147 (1975) 45-53.
Reference
4  [PMID:12156]
  Authors
Chang WJ, Horiuchi S, Takahashi K, Yamasaki M, Yamada Y.
  Title
The structure and function of acid proteases. VI. Effects of acid protease-specific inhibitors on the acid proteases from Aspergillus niger var. macrosporus.
  Journal
J. Biochem. (Tokyo). 80 (1976) 975-81.
Reference
5  [PMID:21699]
  Authors
Tanaka N, Takeuchi M, Ichishima E.
  Title
Purification of an acid proteinase from Aspergillus saitoi and determination of peptide bond specificity.
  Journal
Biochim. Biophys. Acta. 485 (1977) 406-16.
Reference
6
  Authors
Ostoslavskaya, V.I., Kotlova, E.K., Stepanov, V.M., Rudenskaya, G.H., Baratova, L.A. and Belyanova, L.P.
  Title
Aspergillopepsin F-A carboxylic proteinase from Aspergillus foetidus.
  Journal
Bioorg. Khim. 5 (1976) 595-603.
Reference
7  [PMID:7024192]
  Authors
Panneerselvam M, Dhar SC.
  Title
Studies on the peptide bond specificity and the essential groups of an acid proteinase from Aspergillus fumigatus.
  Journal
Ital. J. Biochem. 30 (1981) 207-16.
Reference
8
  Authors
Ostoslavskaya, V.I., Revina, L.P., Kotlova, E.K., Surova, I.A., Levin, E.D., Timokhima, E.A. and Stepanov, V.M.
  Title
The primary structure of aspergillopepsin A, aspartic proteinase from Aspergillus awamori. IV. Amino acid sequence of the enzyme.
  Journal
Bioorg. Khim. 12 (1986) 1030-1047.
Reference
9
  Authors
Yagi, F., Fan, J., Tadera, K. and Kobayashi, A.
  Title
Purification and characterization of carboxyl proteinase from Aspergillus kawachii.
  Journal
Agric. Biol. Chem. 50 (1986) 1029-1033.
Reference
10
  Authors
Majima, E., Oda, K., Murao, S. and Ichishima, E.
  Title
Comparative study on the specificities of several fungal aspartic and acidic proteinases towards the tetradecapeptide of a renin substrate.
  Journal
Agric. Biol. Chem. 52 (1988) 787-793.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
9025-49-4

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