KEGG   ENZYME: 3.4.24.21Help
Entry
EC 3.4.24.21                Enzyme                                 

Name
astacin;
Astacus proteinase;
crayfish small-molecule proteinase
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Metalloendopeptidases
BRITE hierarchy
Reaction(IUBMB)
Hydrolysis of peptide bonds in substrates containing five or more amino acids, preferentially with Ala in P1', and Pro in P2'
Comment
A 22.6 kDa digestive endopeptidase from the cardia of the crayfish Astacus fluviatilis. Type example of peptidase family M12.
History
EC 3.4.24.21 created 1972 as EC 3.4.99.6, transferred 1992 to EC 3.4.24.21
Orthology
K08076  
astacin
Genes
CEL: 
CELE_4R79.1(nas-6) CELE_C07D10.4(nas-7) CELE_C24F3.3(nas-12) CELE_C26C6.3(nas-36) CELE_C34D4.9(nas-8) CELE_C37H5.9(nas-9) CELE_F09E8.6(nas-14) CELE_F38E9.2(nas-39) CELE_F40E10.1(hch-1) CELE_F42A10.8(nas-28) CELE_F45G2.1(nas-1) CELE_F46C5.3(nas-25) CELE_F57C12.1(nas-38) CELE_F58A6.4(nas-29) CELE_K03B8.1(nas-16) CELE_K03B8.2(nas-17) CELE_K03B8.3(nas-18) CELE_K04E7.3(nas-33) CELE_K06A4.1(nas-3) CELE_K09C8.3(nas-10) CELE_T02B11.7(nas-32) CELE_T04G9.2(nas-15) CELE_T11F9.3(nas-20) CELE_T23F4.4(nas-27) CELE_T23H4.3(nas-5) CELE_T24A11.3(toh-1)
CBR: 
CBG00430(Cbr-nas-6)
RDE: 
Taxonomy
Reference
1  [PMID:7041692]
  Authors
Krauhs E, Dorsam H, Little M, Zwilling R, Ponstingl H.
  Title
A protease from Astacus fluviatilis as an aid in protein sequencing.
  Journal
Anal. Biochem. 119 (1982) 153-7.
Reference
2  [PMID:3548817]
  Authors
Titani K, Torff HJ, Hormel S, Kumar S, Walsh KA, Rodl J, Neurath H, Zwilling R.
  Title
Amino acid sequence of a unique protease from the crayfish Astacus fluviatilis.
  Journal
Biochemistry. 26 (1987) 222-6.
  Sequence
Reference
3
  Authors
Stocker, W., Wolz, R.L., Zwilling, R., Strydom, D.J. and Auld, D.S.
  Title
Astacus protease, a zinc metalloenzyme.
  Journal
Biochemistry 27 (1988) 5026-5032.
Reference
4  [PMID:2261483]
  Authors
Stocker W, Ng M, Auld DS.
  Title
Fluorescent oligopeptide substrates for kinetic characterization of the specificity of Astacus protease.
  Journal
Biochemistry. 29 (1990) 10418-25.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
143179-21-9

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