KEGG   ENZYME: 3.5.1.46Help
Entry
EC 3.5.1.46                 Enzyme                                 

Name
6-aminohexanoate-oligomer exohydrolase;
6-aminohexanoate-dimer hydrolase;
nylB (gene name);
6-aminohexanoic acid oligomer hydrolase (ambiguous);
N-(6-aminohexanoyl)-6-aminohexanoate amidohydrolase;
nylon-6 hydrolase (ambiguous)
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides
BRITE hierarchy
Sysname
N-(6-aminohexanoyl)-6-aminohexanoate exoamidohydrolase
Reaction(IUBMB)
(1) [N-(6-aminohexanoyl)]n + H2O = [N-(6-aminohexanoyl)]n-1 + 6-aminohexanoate [RN:R10975];
(2) N-(6-aminohexanoyl)-6-aminohexanoate + H2O = 2 6-aminohexanoate [RN:R00059]
Reaction(KEGG)
R00059 R10975;
(other) R10979
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Substrate
[N-(6-aminohexanoyl)]n [CPD:C20988];
H2O [CPD:C00001];
N-(6-aminohexanoyl)-6-aminohexanoate [CPD:C01255]
Product
[N-(6-aminohexanoyl)]n-1;
6-aminohexanoate [CPD:C02378]
Comment
The enzyme is involved in degradation of nylon-6 oligomers. It degrades linear oligomers of 6-aminohexanoate with a degree of polymerization of 2--20 by exo-type cleavage, removing residues sequentially from the N-terminus. Activity decreases with the increase of the polymerization number of the oligomer. cf. EC 3.5.1.117, 6-aminohexanoate-oligomer endohydrolase and EC 3.5.2.12, 6-aminohexanoate-cyclic-dimer hydrolase.
History
EC 3.5.1.46 created 1983, modified 2014
Pathway
Caprolactam degradation
Microbial metabolism in diverse environments
Orthology
K01453  
6-aminohexanoate-oligomer exohydrolase
Genes
ARR: 
AAU: 
AAur_3953(nylB)
Taxonomy
Reference
1  [PMID:7262074]
  Authors
Kinoshita S, Terada T, Taniguchi T, Takene Y, Masuda S, Matsunaga N, Okada H.
  Title
Purification and characterization of 6-aminohexanoic-acid-oligomer hydrolase of Flavobacterium sp. Ki72.
  Journal
Eur. J. Biochem. 116 (1981) 547-51.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
UM-BBD (Biocatalysis/Biodegradation Database): 
BRENDA, the Enzyme Database: 
CAS: 
75216-15-8

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