KEGG   ENZYME: 3.5.1.81Help
Entry
EC 3.5.1.81                 Enzyme                                 
Name
N-acyl-D-amino-acid deacylase
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides
BRITE hierarchy
Sysname
N-acyl-D-amino acid amidohydrolase
Reaction(IUBMB)
N-acyl-D-amino acid + H2O = a carboxylate + D-amino acid [RN:R02192]
Reaction(KEGG)
Substrate
N-acyl-D-amino acid [CPD:C06378];
H2O [CPD:C00001]
Product
carboxylate [CPD:C00060];
D-amino acid [CPD:C00405]
Comment
The enzyme from Alcaligenes denitrificans subsp. xylosoxydans and Alcaligenes xylosoxydans subsp. xylosoxydans has wide specificity; hydrolyses N-acyl derivative of neutral D-amino acids. Used in separating D- and L-amino acids. Requires zinc.
Orthology
K06015  
N-acyl-D-amino-acid deacylase
Genes
ECM: 
EFE: 
EAE: 
EAR: 
KPN: 
KPU: 
KPM: 
KPP: 
KPO: 
KOX: 
CRO: 
ROD_48401
SPE: 
Spro_0551
SMAF: 
D781_2939
PAO: 
Pat9b_4339
ROR: 
RORB6_15460
RHD: 
R2APBS1_3299 R2APBS1_3302
VVM: 
VVMO6_04053
VHA: 
VIBHAR_06353
VSP: 
VS_II0036
VFU: 
vfu_A02213
PPUU: 
PputUW4_00618
PSP: 
PSPPH_0405
PFO: 
Pfl01_0723
PFS: 
PFLU5178
PFE: 
PSF113_0729
PFC: 
PflA506_4471
PBA: 
PSEBR_a701
SHL: 
Shal_3762
CSA: 
Csal_0091
RSL: 
RPSI07_mp1217
RSN: 
RSPO_m00833(ndeD)
RPI: 
Rpic_4195
RPF: 
Rpic12D_4305
REU: 
Reut_B4020
REH: 
H16_B0531 H16_B1123 H16_B2124
RME: 
Rmet_4772 Rmet_5093
CTI: 
RALTA_B1120 RALTA_B1823
CNC: 
CNE_2c10850(dan1) CNE_2c11800(dan2) CNE_2c20900
BMA: 
BMA0178
BMV: 
BMASAVP1_A2770
BML: 
BMA10229_A2309
BMN: 
BMA10247_2389
BPS: 
BPSL0630(dan)
BPM: 
BURPS1710b_0837
BPL: 
BURPS1106A_0674
BPD: 
BURPS668_0658
BPR: 
GBP346_A0586
BPZ: 
BP1026B_I2895(dan)
BPQ: 
BPC006_I0660
BTE: 
BTH_I0546
BVI: 
Bcep1808_2845
BUR: 
Bcep18194_A6068
BCN: 
Bcen_2128 Bcen_3337
BCH: 
Bcen2424_2740 Bcen2424_5030
BCM: 
Bcenmc03_2766 Bcenmc03_5254
BCJ: 
BCAL0866(dan) BCAM2259(dan)
BAM: 
Bamb_2790 Bamb_4439
BAC: 
BamMC406_2657 BamMC406_4959
BMU: 
Bmul_0559 Bmul_6029
BMJ: 
BMULJ_02702 BMULJ_05498(dan)
BCT: 
GEM_0702
BPH: 
Bphy_0366
BPY: 
Bphyt_0641 Bphyt_3714
BGL: 
bglu_1g30900
BUG: 
BC1001_0350
BGE: 
BC1002_0361
BGF: 
BC1003_0361
BRH: 
RBRH_02112
BGD: 
bgla_1g34490
BYI: 
BYI23_A002870
BUK: 
MYA_2498
BPX: 
BUPH_04243
BBH: 
BN112_3094(ptpX)
BPT: 
Bpet0765 Bpet0806 Bpet1578 Bpet2327
BAV: 
BAV0959(dan)
AXY: 
AXYL_01207 AXYL_01306 AXYL_03308
AKA: 
TKWG_19395
RFR: 
Rfer_0927 Rfer_0978
POL: 
Bpro_0663 Bpro_4816
AAA: 
Acav_1137
VEI: 
Veis_1862 Veis_3331
VAP: 
Vapar_1221 Vapar_1551
VPE: 
Varpa_1303 Varpa_1698
RTA: 
Rta_12410 Rta_14020 Rta_27230
HSE: 
Hsero_0570(ndeD) Hsero_1750(ndeD)
CFU: 
CFU_1275(dan)
GEO: 
Geob_0285
DDE: 
Dde_1269
DDN: 
DND132_1353
DPI: 
BN4_11190
SCL: 
sce1547
BRA: 
BRADO6610(dan)
BBT: 
BBta_0926(dan)
AOL: 
S58_09940
CAK: 
Caul_1130
JAN: 
Jann_3245
SAL: 
Sala_1467
SPHM: 
G432_10805
SSY: 
SLG_14230 SLG_14240
ALI: 
AZOLI_p20308
BCZ: 
BCZK4241 BCZK4242
PMS: 
KNP414_06947
PMQ: 
PM3016_6541
PMW: 
B2K_33055
CBE: 
Cbei_4937
CPY: 
Cphy_1501
CLJ: 
CLJU_c21720 CLJU_c29960
CSH: 
Closa_2960
CSR: 
Cspa_c28990
CDF: 
CD630_31820
CDC: 
CD196_2992
CDL: 
CDR20291_0603 CDR20291_3038
CDG: 
CDBI1_03210 CDBI1_15545
DSY: 
DSY1804
DHD: 
Dhaf_2958
DDH: 
Desde_2406
DRM: 
Dred_0263
DOR: 
Desor_2016
DAI: 
Desaci_1133
DMI: 
Desmer_1778
TMR: 
Tmar_2329
BPRS: 
CK3_17970
TEP: 
TepRe1_1859
TAE: 
TEPIRE1_21500
TPZ: 
Tph_c27030(dan2)
HAS: 
Halsa_1184
AAR: 
Acear_0086
HHL: 
Halha_0096
AFN: 
Acfer_1991
MSM: 
MSMEG_3787
MSG: 
MSMEI_1155 MSMEI_3699
NBR: 
O3I_013240
RHA: 
RHA1_ro02461 RHA1_ro02463
ROP: 
ROP_21890
REQ: 
REQ_04900
TPR: 
Tpau_3006
SCO: 
SCO4986(2SCK36.09c)
SMA: 
SAV_3282
SGR: 
SGR_2547
SCB: 
SCAB_33971
SSX: 
SACTE_4239
SVL: 
Strvi_1299 Strvi_4472
SCT: 
SCAT_3867
SCY: 
SCATT_38530
SFA: 
Sfla_2279
SBH: 
SBI_04231
SHY: 
SHJG_6091
SHO: 
SHJGH_5853
SVE: 
SVEN_4666
SDV: 
BN159_3408
SALB: 
XNR_4068
STRP: 
F750_4526
KSK: 
KSE_64900
AAU: 
AAur_4061
ARR: 
ARUE_c42040(dan)
RSA: 
RSal33209_0833
MPH: 
MLP_33450
KFL: 
Kfla_6164
NDA: 
Ndas_5490
NAL: 
B005_1609
KRA: 
Krad_1032
SEN: 
SACE_2583 SACE_3585 SACE_5301
AMD: 
AMED_6064
AMN: 
RAM_31090
AMM: 
AMES_5979
PDX: 
Psed_3083
AMI: 
Amir_5481
SESP: 
BN6_66240
CAI: 
Caci_0720
SNA: 
Snas_2530
TPI: 
TREPR_2511
SBU: 
SpiBuddy_0936
SGP: 
SpiGrapes_1056
ABA: 
Acid345_2609
GMA: 
AciX8_2260
CTM: 
Cabther_B0468
RMR: 
Rmar_1358
NKO: 
Niako_5085 Niako_6457
SCN: 
Solca_2821
HHY: 
Halhy_0907 Halhy_1357
CMR: 
Cycma_3795
EVI: 
Echvi_1083
LBY: 
Lbys_1896
RSI: 
Runsl_3134
EOL: 
Emtol_1353 Emtol_3072
FAE: 
FAES_5424
MRS: 
Murru_3303
IPO: 
Ilyop_0127
RBA: 
RB2098(ndaD)
PSL: 
Psta_1927
IPA: 
Isop_0277
SACI: 
Sinac_3600 Sinac_5344
ACO: 
Amico_0202 Amico_0205 Amico_0211
TLI: 
Tlie_0010
AMO: 
Anamo_0547 Anamo_1957
SBR: 
SY1_18430 SY1_23340
CAP: 
CLDAP_05670 CLDAP_30820
DDR: 
Deide_3p00232
TTA: 
Theth_1319
MOX: 
DAMO_1463(dan)
HHI: 
HAH_5304(ureX)
NMG: 
Nmag_3660
PYN: 
PNA2_0671
HAH: 
Halar_3331 Halar_3345
 » show all
Taxonomy
Reference
1  [PMID:8541651]
  Authors
Wakayama M, Katsuno Y, Hayashi S, Miyamoto Y, Sakai K, Moriguchi M.
  Title
Cloning and sequencing of a gene encoding D-aminoacylase from Alcaligenes xylosoxydans subsp. xylosoxydans A-6 and expression of the gene in Escherichia coli.
  Journal
Biosci. Biotechnol. Biochem. 59 (1995) 2115-9.
  Organism
Alcaligenes xylosoxydans
Reference
2  [PMID:8776758]
  Authors
Wakayama M, Hayashi S, Yatsuda Y, Katsuno Y, Sakai K, Moriguchi M.
  Title
Overproduction of D-aminoacylase from Alcaligenes xylosoxydans subsp. xylosoxydans A-6 in Escherichia coli and its purification.
  Journal
Protein. Expr. Purif. 7 (1996) 395-9.
  Organism
Alcaligenes xylosoxydans
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
65979-42-2
DBGET integrated database retrieval system