KEGG   ENZYME: 3.6.1.5Help
Entry
EC 3.6.1.5                  Enzyme                                 

Name
apyrase;
ATP-diphosphatase;
adenosine diphosphatase;
ADPase;
ATP diphosphohydrolase [ambiguous]
Class
Hydrolases;
Acting on acid anhydrides;
In phosphorus-containing anhydrides
BRITE hierarchy
Sysname
nucleoside triphosphate phosphohydrolase (nucleoside monophosphoate-forming)
Reaction(IUBMB)
a nucleoside 5'-triphosphate + 2 H2O = a nucleoside 5'-phosphate + 2 phosphate (overall reaction) [RN:R10443];
(1a) a nucleoside 5'-triphosphate + H2O = a nucleoside 5'-diphosphate + phosphate [RN:R02319];
(1b) a nucleoside 5'-diphosphate + H2O = a nucleoside 5'-phosphate + phosphate [RN:R10442]
Reaction(KEGG)
Substrate
nucleoside 5'-triphosphate [CPD:C00201];
H2O [CPD:C00001];
nucleoside 5'-diphosphate [CPD:C00454]
Product
nucleoside 5'-phosphate [CPD:C01117];
phosphate [CPD:C00009];
nucleoside 5'-diphosphate [CPD:C00454]
Comment
Apyrases are active against both di- and triphosphate nucleotides (NDPs and NTPs) and hydrolyse NTPs to nucleotide monophosphates (NMPs) in two distinct successive phosphate-releasing steps, with NDPs as intermediates. They differ from ATPases, which specifically hydrolyse ATP, by hydrolysing both ATP and ADP. The eukaryotic enzymes requires Ca2+, but Mg2+ can substitute. Most of the ecto-ATPases that occur on the cell surface and hydrolyse extracellular nucleotides belong to this enzyme family.
History
EC 3.6.1.5 created 1961, modified 1976, modified 2000, modified 2013
Pathway
Purine metabolism
Pyrimidine metabolism
Orthology
K01510  
apyrase
K14641  
apyrase
K14642  
golgi apyrase
Genes
HSA: 
377841(ENTPD8) 953(ENTPD1) 956(ENTPD3)
PTR: 
100614135(ENTPD1) 460286(ENTPD3) 736974(ENTPD8)
PPS: 
100971059(ENTPD1) 100983594(ENTPD3) 100995976(ENTPD8)
GGO: 
101132716(ENTPD1) 101132747(ENTPD3)
PON: 
100442938(ENTPD8) 100447406(ENTPD3) 100453241(ENTPD1)
MCC: 
703591(ENTPD1) 704424(ENTPD8) 718311(ENTPD3)
MCF: 
102134382(ENTPD8) 102138702(ENTPD3)
MMU: 
12495(Entpd1) 215446(Entpd3) 72090(Entpd8)
RNO: 
316077(Entpd3) 613267(Entpd8) 64519(Entpd1)
CGE: 
100755122(Entpd8) 100762593(Entpd1) 100772668(Entpd3)
HGL: 
101696417(Entpd3) 101708535(Entpd8) 101715301(Entpd1)
TUP: 
102469337(ENTPD3) 102473460(ENTPD1) 102475046(ENTPD8)
CFA: 
100688937(ENTPD8) 485604(ENTPD3) 486810(ENTPD1)
AML: 
FCA: 
101085582(ENTPD8) 101096356(ENTPD1) 101098083(ENTPD3)
PTG: 
102953346(ENTPD8) 102960243(ENTPD1) 102962344(ENTPD3)
BTA: 
282223(ENTPD1) 506087(ENTPD3) 515532(ENTPD8)
BOM: 
102269229(ENTPD8) 102270483(ENTPD1) 102273587(ENTPD3)
PHD: 
102321352(ENTPD1) 102338946(ENTPD3)
CHX: 
102189182(ENTPD1) 102190080(ENTPD3)
SSC: 
100516719(ENTPD8) 100623360(ENTPD3) 397298(ENTPD1)
CFR: 
102503442(ENTPD1) 102509620(ENTPD3) 102520072(ENTPD8)
BACU: 
103006116(ENTPD8) 103014786(ENTPD1) 103018820(ENTPD3)
LVE: 
103069223(ENTPD3) 103078660(ENTPD1) 103080594(ENTPD8)
ECB: 
100058558(ENTPD8) 100068004(ENTPD3) 100070956(ENTPD1)
MYB: 
102243699(ENTPD8) 102255350(ENTPD3) 102256383(ENTPD1)
MYD: 
102752806(ENTPD1) 102755153(ENTPD8) 102774497(ENTPD3)
PALE: 
102879249(ENTPD3) 102890548(ENTPD1) 102897950(ENTPD8)
MDO: 
100022843(ENTPD8) 100022899(ENTPD1) 100023686 100025436(ENTPD3)
SHR: 
100914154(ENTPD1) 100919726 100926510(ENTPD3) 100932670(ENTPD8)
OAA: 
100078824(ENTPD3)
GGA: 
374095(ENTPD8) 417281(ENTPD8L) 423826(ENTPD1) 427778(ENTPD1) 428447(ENTPD3)
MGP: 
TGU: 
100218994(ENTPD1) 100221575(ENTPD3) 100226299(ENTPD1) 100229162
FAB: 
PHI: 
APLA: 
FPG: 
FCH: 
CLV: 
ASN: 
AMJ: 
PSS: 
CMY: 
ACS: 
PBI: 
103052703(ENTPD3) 103053833(ENTPD1) 103066259(ENTPD8)
XLA: 
444164(entpd1)
XTR: 
100125010(entpd3) 448501(entpd1) 733458(entpd8)
DRE: 
100005551(entpd3) 436652(entpd8) 445151(entpd1)
TRU: 
MZE: 
OLA: 
XMA: 
LCM: 
102348651(ENTPD3) 102360392(ENTPD8) 102367063
CMK: 
BFO: 
TSP: 
SMM: 
HMG: 
AQU: 
ATH: 
ALY: 
CRB: 
EUS: 
CIT: 
CIC: 
TCC: 
GMX: 
PVU: 
MTR: 
CAM: 
FVE: 
PPER: 
CSV: 
RCU: 
POP: 
POPTR_0004s20630g POPTR_0008s08600g(POPTRDRAFT_765739) POPTR_0009s15920g(POPTRDRAFT_878226) POPTR_0010s17620g(POPTRDRAFT_833712) POPTR_0013s05040g(POPTRDRAFT_729947) POPTR_0013s05060g POPTR_0019s04650g(POPTRDRAFT_809518) POPTR_0019s04670g(POPTRDRAFT_573883) POPTR_0671s00200g
VVI: 
SLY: 
SOT: 
OSA: 
DOSA: 
Os03t0378000-01(Os03g0378000) Os07t0682800-01(Os07g0682800) Os08t0436100-01(Os08g0436100) Os11t0125900-00(Os11g0125900) Os11t0126400-00(Os11g0126400) Os11t0439600-01(Os11g0439600) Os11t0440200-00(Os11g0440200) Os12t0123500-01(Os12g0123500)
OBR: 
BDI: 
SBI: 
SORBI_01g034030(SORBIDRAFT_01g034030) SORBI_01g036510(SORBIDRAFT_01g036510) SORBI_05g001550(SORBIDRAFT_05g001550) SORBI_05g001560(SORBIDRAFT_05g001560) SORBI_05g008570(SORBIDRAFT_05g008570) SORBI_07g021350(SORBIDRAFT_07g021350) SORBI_08g001950(SORBIDRAFT_08g001950) SORBI_08g001970(SORBIDRAFT_08g001970)
ZMA: 
SITA: 
ATR: 
s00007p00221730(AMTR_s00007p00221730) s00010p00262060(AMTR_s00010p00262060) s00119p00065770(AMTR_s00119p00065770)
SMO: 
PPP: 
CRE: 
VCN: 
OLU: 
OTA: 
BPG: 
MIS: 
MPP: 
CSL: 
SCE: 
YER005W(YND1)
AGO: 
ERC: 
KLA: 
LTH: 
PPA: 
VPO: 
ZRO: 
CGR: 
NCS: 
NCAS_0I00760(NCAS0I00760)
NDI: 
NDAI_0A08280(NDAI0A08280)
TPF: 
TPHA_0L00770(TPHA0L00770)
TBL: 
TBLA_0A01060(TBLA0A01060)
TDL: 
TDEL_0H03120(TDEL0H03120)
KAF: 
KAFR_0L00600(KAFR0L00600)
DHA: 
PIC: 
PGU: 
SPAA: 
LEL: 
CAL: 
CTP: 
COT: 
CDU: 
CTEN: 
YLI: 
CLU: 
MBR: 
PFA: 
TET: 
PTM: 
EHX: 
GTT: 
TVA: 
LPN: 
LPH: 
LPO: 
LPU: 
LPM: 
LPF: 
LPP: 
LPC: 
LPA: 
LPE: 
 » show all
Taxonomy
Reference
1  [PMID:14953432]
  Authors
KRISHNAN PS.
  Title
Apyrase, pyrophosphatase and metaphosphatase of Penicillium chrysogenum.
  Journal
Arch. Biochem. 37 (1952) 224-34.
  Organism
Penicillium chrysogenum
Reference
2  [PMID:13930517]
  Authors
LIEBECQ C, LALLEMAND A, DEGUELDRE-GUILLAUME MJ
  Title
[Partial purification and properties of potato apyrase.]
  Journal
Bull. Soc. Chim. Biol. (Paris). 45 (1963) 573-94.
Reference
3  [PMID:3038893]
  Authors
Chen YR, Datta N, Roux SJ
  Title
Purification and partial characterization of a calmodulin-stimulated nucleoside triphosphatase from pea nuclei.
  Journal
J. Biol. Chem. 262 (1987) 10689-94.
Reference
4  [PMID:8529670]
  Authors
Christoforidis S, Papamarcaki T, Galaris D, Kellner R, Tsolas O
  Title
Purification and properties of human placental ATP diphosphohydrolase.
  Journal
Eur. J. Biochem. 234 (1995) 66-74.
  Organism
Homo sapiens
  Sequence
[hsa:953]
Reference
5  [PMID:8626624]
  Authors
Wang TF, Guidotti G
  Title
CD39 is an ecto-(Ca2+,Mg2+)-apyrase.
  Journal
J. Biol. Chem. 271 (1996) 9898-901.
  Organism
Homo sapiens
  Sequence
[hsa:953]
Reference
6  [PMID:10409709]
  Authors
Gao XD, Kaigorodov V, Jigami Y
  Title
YND1, a homologue of GDA1, encodes membrane-bound apyrase required for Golgi N- and O-glycosylation in Saccharomyces cerevisiae.
  Journal
J. Biol. Chem. 274 (1999) 21450-6.
  Organism
Saccharomyces cerevisiae
  Sequence
[sce:YER005W]
Reference
7  [PMID:15576788]
  Authors
Xu W, Jones CR, Dunn CA, Bessman MJ
  Title
Gene ytkD of Bacillus subtilis encodes an atypical nucleoside triphosphatase member of the Nudix hydrolase superfamily.
  Journal
J. Bacteriol. 186 (2004) 8380-4.
  Organism
Bacillus subtilis
  Sequence
[bsu:BSU30630]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
9000-95-7

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