KEGG   ENZYME: 4.1.3.17Help
Entry
EC 4.1.3.17                 Enzyme                                 

Name
4-hydroxy-4-methyl-2-oxoglutarate aldolase;
pyruvate aldolase;
gamma-methyl-gamma-hydroxy-alpha-ketoglutaric aldolase;
4-hydroxy-4-methyl-2-ketoglutarate aldolase;
4-hydroxy-4-methyl-2-oxoglutarate pyruvate-lyase;
HMG aldolase;
CHA aldolase;
4-carboxy-4-hydroxy-2-oxoadipate aldolase
Class
Lyases;
Carbon-carbon lyases;
Oxo-acid-lyases
BRITE hierarchy
Sysname
4-hydroxy-4-methyl-2-oxoglutarate pyruvate-lyase (pyruvate-forming)
Reaction(IUBMB)
(1) 4-hydroxy-4-methyl-2-oxoglutarate = 2 pyruvate [RN:R00008];
(2) 2-hydroxy-4-oxobutane-1,2,4-tricarboxylate = oxaloacetate + pyruvate [RN:R00350]
Reaction(KEGG)
Substrate
4-hydroxy-4-methyl-2-oxoglutarate [CPD:C06033];
2-hydroxy-4-oxobutane-1,2,4-tricarboxylate [CPD:C04115]
Product
pyruvate [CPD:C00022];
oxaloacetate [CPD:C00036]
Comment
Requires a divalent metal ion [3]. This enzyme participates in the degradation of protocatechuate (via the meta-cleavage pathway), phthalate, syringate and gallate [1-3]. The enzyme from Pseudomonas ochraceae can also cleave 4-hydroxy-2-oxoglutarate to glyoxylate and pyruvate, and also catalyses the reaction of EC 4.1.1.3 (oxaloacetate decarboxylase) [3].
History
EC 4.1.3.17 created 1972, modified 2012
Pathway
Benzoate degradation
C5-Branched dibasic acid metabolism
Microbial metabolism in diverse environments
Orthology
K10218  
4-hydroxy-4-methyl-2-oxoglutarate aldolase
Genes
YPE: 
YPK: 
YPA: 
YPN: 
YPP: 
YPG: 
YPZ: 
YPT: 
YPD: 
YPX: 
YPH: 
YPS: 
YPI: 
YPY: 
YPB: 
EAM: 
EAMY_1950(fldZ)
EAY: 
EBI: 
SOD: 
KPN: 
KPU: 
KPM: 
KPP: 
KPE: 
KPK_0045(ligK)
KPO: 
KPR: 
KPJ: 
KPI: 
KVA: 
CRO: 
SPE: 
SMAF: 
SERR: 
SFO: 
PAO: 
RAH: 
RAA: 
ROR: 
XCC: 
XCC4030(fldZ)
XCB: 
XCA: 
XCP: 
XCR_0230(ligK)
XCV: 
XAC: 
XAC4155(fldZ)
XCI: 
XAX: 
XAO: 
XOO: 
XOO4565(fldZ)
XOM: 
XOP: 
PXO_03235(ligK)
XOR: 
XOC_0386(ligK)
XFU: 
VEJ: 
PPU: 
PPF: 
PPW: 
PPB: 
PPI: 
PPX: 
PPUT: 
PPUN: 
PPUU: 
PFE: 
AVN: 
AVL: 
AVD: 
PAT: 
MBS: 
CSA: 
MMW: 
MME: 
MPC: 
PSE: 
RSO: 
RS03175(RSp1231)
RSL: 
RSN: 
RSM: 
RSE: 
REU: 
CNC: 
RME: 
BUR: 
BMU: 
BMJ: 
BPH: 
BGE: 
BGF: 
BGD: 
BYI: 
BPX: 
BUO: 
PPK: 
PPNO: 
PRB: 
BBM: 
BPT: 
BHO: 
D560_0869(ligK)
CDN: 
RFR: 
POL: 
PNA: 
ACK: 
DAC: 
DEL: 
VAP: 
VPE: 
VPD: 
CTT: 
ADN: 
ADK: 
RTA: 
HSE: 
LCH: 
AZO: 
APP: 
DTI: 
SME: 
SMK: 
SMQ: 
SMX: 
SMEG: 
SMEL: 
RHI: 
SFH: 
ATU: 
Atu4285(menG)
ARA: 
Arad_2915(ligK) Arad_7492(menG)
AVI: 
Avi_7652(menG)
AGR: 
RET: 
REL: 
RLE: 
RLT: 
RLG: 
RLB: 
RLU: 
RIR: 
RHL: 
BJA: 
BJU: 
BRA: 
BRADO2338(ligK)
BBT: 
BBta_2698(ligK)
BRS: 
AOL: 
RPA: 
RPA4698(FldZ)
RPB: 
RPD: 
RPE: 
RPT: 
RPX: 
AZC: 
PHL: 
BSB: 
AEX: 
RSP: 
RSH: 
RSK: 
PAMI: 
KVU: 
KVL: 
PGA: 
NAR: 
NPP: 
SWI: 
SJP: 
SCH: 
SSY: 
MAG: 
AZL: 
ALI: 
ABS: 
PGV: 
APB: 
GCT: 
AAC: 
AAD: 
TC41_1506(hps-phi)
ECAS: 
TPZ: 
MSM: 
MSG: 
MRH: 
RHA: 
RER: 
ROP: 
ROA: 
GPO: 
GOR: 
SMA: 
SSX: 
SVL: 
SFA: 
SBH: 
SALB: 
STRP: 
SRC: 
KSK: 
KSE_75160(pcmE)
ART: 
AAU: 
ACH: 
APN: 
ARR: 
MPH: 
SRO: 
BSD: 
MMAR: 
SVI: 
PDX: 
MAU: 
ACTN: 
RXY: 
CWO: 
SUS: 
SLI: 
RSI: 
EOL: 
TRO: 
STI: 
TSC: 
THC: 
TOS: 
MRB: 
MRE: 
SACS: 
MSE: 
 » show all
Taxonomy
Reference
1  [PMID:5076765]
  Authors
Tack BF, Chapman PJ, Dagley S.
  Title
Purification and properties of 4-hydroxy-4-methyl-2-oxoglutarate aldolase.
  Journal
J. Biol. Chem. 247 (1972) 6444-9.
  Organism
Pseudomonas putida
Reference
2
  Authors
Wood, W.A.
  Title
2-Keto-3-deoxy-6-phosphogluconic and related aldolases.
  Journal
In: Boyer, P.D. (Ed.), The Enzymes, 3rd ed., vol. 7, Academic Press, New York, 1972, p. 281-302.
Reference
3  [PMID:6841353]
  Authors
Maruyama K.
  Title
Purification and properties of 2-pyrone-4,6-dicarboxylate hydrolase.
  Journal
J. Biochem. (Tokyo). 93 (1983) 557-65.
  Organism
Pseudomonas ochraceae
Reference
4  [PMID:21219457]
  Authors
Nogales J, Canales A, Jimenez-Barbero J, Serra B, Pingarron JM, Garcia JL, Diaz E
  Title
Unravelling the gallic acid degradation pathway in bacteria: the gal cluster from Pseudomonas putida.
  Journal
Mol. Microbiol. 79 (2011) 359-74.
  Organism
Pseudomonas putida
  Sequence
[ppu:PP_2514]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
37290-65-6

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