4-hydroxy-4-methyl-2-oxoglutarate aldolase;
pyruvate aldolase;
gamma-methyl-gamma-hydroxy-alpha-ketoglutaric aldolase;
4-hydroxy-4-methyl-2-ketoglutarate aldolase;
4-hydroxy-4-methyl-2-oxoglutarate pyruvate-lyase;
HMG aldolase;
CHA aldolase;
4-carboxy-4-hydroxy-2-oxoadipate aldolase

Lyases;
Carbon-carbon lyases;
Oxo-acid-lyases

4-hydroxy-4-methyl-2-oxoglutarate pyruvate-lyase (pyruvate-forming)

(1) 4-hydroxy-4-methyl-2-oxoglutarate = 2 pyruvate [RN:R00008];
(2) 2-hydroxy-4-oxobutane-1,2,4-tricarboxylate = oxaloacetate + pyruvate [RN:R00350]

R00008 R00350

4-hydroxy-4-methyl-2-oxoglutarate [CPD:C06033];
2-hydroxy-4-oxobutane-1,2,4-tricarboxylate [CPD:C04115]

Requires a divalent metal ion [3]. This enzyme participates in the degradation of protocatechuate (via the meta-cleavage pathway), phthalate, syringate and gallate [1-3]. The enzyme from Pseudomonas ochraceae can also cleave 4-hydroxy-2-oxoglutarate to glyoxylate and pyruvate, and also catalyses the reaction of EC 4.1.1.3 (oxaloacetate decarboxylase) [3].

1  [PMID:5076765]

Tack BF, Chapman PJ, Dagley S.

Purification and properties of 4-hydroxy-4-methyl-2-oxoglutarate aldolase.

J. Biol. Chem. 247 (1972) 6444-9.

Pseudomonas putida

2

Wood, W.A.

2-Keto-3-deoxy-6-phosphogluconic and related aldolases.

In: Boyer, P.D. (Ed.), The Enzymes, 3rd ed., vol. 7, Academic Press, New York, 1972, p. 281-302.

3  [PMID:6841353]

Maruyama K.

Purification and properties of 2-pyrone-4,6-dicarboxylate hydrolase.

J. Biochem. (Tokyo). 93 (1983) 557-65.

Pseudomonas ochraceae

4  [PMID:21219457]

Nogales J, Canales A, Jimenez-Barbero J, Serra B, Pingarron JM, Garcia JL, Diaz E

Unravelling the gallic acid degradation pathway in bacteria: the gal cluster from Pseudomonas putida.

Mol. Microbiol. 79 (2011) 359-74.