KEGG   ENZYME: 4.1.3.39Help
Entry
EC 4.1.3.39                 Enzyme                                 
Name
4-hydroxy-2-oxovalerate aldolase;
4-hydroxy-2-ketovalerate aldolase;
HOA;
DmpG;
4-hydroxy-2-oxovalerate pyruvate-lyase;
4-hydroxy-2-oxopentanoate pyruvate-lyase
Class
Lyases;
Carbon-carbon lyases;
Oxo-acid-lyases
BRITE hierarchy
Sysname
4-hydroxy-2-oxopentanoate pyruvate-lyase (acetaldehyde-forming)
Reaction(IUBMB)
4-hydroxy-2-oxopentanoate = acetaldehyde + pyruvate [RN:R00750]
Reaction(KEGG)
R00750;
(other) R05298
Show
Substrate
4-hydroxy-2-oxopentanoate [CPD:C03589]
Product
acetaldehyde [CPD:C00084];
pyruvate [CPD:C00022]
Comment
Requires Mn2+ for maximal activity [1]. The enzyme from Pseudomonas putida is also stimulated by the presence of NADH [1]. In Pseudomonas species, this enzyme forms part of a bifunctional enzyme with EC 1.2.1.10, acetaldehyde dehydrogenase (acetylating). It catalyses the penultimate step in the meta-cleavage pathway for the degradation of phenols, methylphenols and catechol [1].
Pathway
Phenylalanine metabolism
Benzoate degradation
Dioxin degradation
Xylene degradation
Metabolic pathways
Microbial metabolism in diverse environments
Orthology
K01666  
4-hydroxy 2-oxovalerate aldolase
Genes
ECO: 
b0352(mhpE)
ECJ: 
Y75_p0341(mhpE)
EBW: 
BWG_0241(mhpE)
ECOK: 
ECE: 
Z0452(mhpE)
ECS: 
ECF: 
ETW: 
ECSP_0416(mhpE)
ELX: 
EOJ: 
EOI: 
ECO111_0388(mhpE)
EOH: 
ECO103_0334(mhpE)
EOK: 
G2583_0465(mhpE)
ELR: 
ECO55CA74_02235
ECX: 
EcHS_A0416(mhpE)
ECW: 
EcE24377A_0376(mhpE)
ECM: 
EcSMS35_0383(mhpE)
ECY: 
ECSE_0377
ECR: 
ECIAI1_0353(mhpE)
ECQ: 
ECED1_0380(mhpE)
ECK: 
EC55989_0359(mhpE)
ECT: 
ECIAI39_0326(mhpE)
EOC: 
CE10_0320(mhpE)
EUM: 
ECUMN_0395(mhpE)
ELO: 
EC042_0389(mhpE)
ELH: 
ETEC_0408
ESE: 
ECSF_0323
ESO: 
O3O_05580
ESM: 
O3M_19700
ESL: 
O3K_19715
ECL: 
EcolC_3273
EBR: 
ECB_00306(mhpE)
EBD: 
ECBD_3305
EKO: 
EKO11_3490
EKF: 
KO11_21820(mhpE)
EDH: 
EcDH1_3254
EDJ: 
ECDH1ME8569_0339(mhpE)
ENA: 
ECNA114_0335(mhpE)
EUN: 
UMNK88_402(mhpE)
ELW: 
ECW_m0430(mhpE)
ELL: 
WFL_02115(mhpE)
ELP: 
P12B_c0370(mhpE)
EBL: 
ECD_00306(mhpE)
EBE: 
B21_00310(mhpE)
ECOA: 
APECO78_05280
EBT: 
EBL_c19990(mhpE)
SSN: 
SSON_0331(mhpE)
SSJ: 
SSON53_01800
ECA: 
ECA1429(nahM)
EPY: 
EpC_04020
EPR: 
EPYR_00420
PLU: 
plu4081
KPN: 
KPN_02117(mhpE)
KPU: 
KP1_3212(mhpE)
KPM: 
KPHS_30990
KPP: 
A79E_2127
KPE: 
KPK_2206(mhpE)
KPO: 
KPN2242_13540
KVA: 
Kvar_2158
KOX: 
KOX_22670
SPE: 
Spro_3025
XNE: 
XNC1_0240
ROR: 
RORB6_04300
EBF: 
D782_1987
PSD: 
DSC_09375
VEJ: 
VEJY3_06805
VFU: 
vfu_A01971
PAF: 
PAM18_3945
PPU: 
PP_1791
PPF: 
Pput_2873 Pput_2888
PPW: 
PputW619_1984 PputW619_2007
PPT: 
PPS_1433
PPX: 
T1E_4258(cmtG) T1E_4274(tobH)
PPUH: 
B479_25865
PPUU: 
PputUW4_01662(mhpE)
PSB: 
Psyr_3072
PSP: 
PSPPH_2158
PEN: 
PSEEN0975
PSC: 
A458_06510
PDR: 
H681_22445
AVN: 
Avin_08740(xylK) Avin_15130(mphE) Avin_30570(lapG) Avin_42120
AVL: 
AvCA_08740(xylK) AvCA_15130(mphE) AvCA_30570(lapG) AvCA_42120
AVD: 
AvCA6_08740(xylK) AvCA6_15130(mphE) AvCA6_30570(lapG) AvCA6_42120
SDN: 
Sden_2668
SPL: 
Spea_2116
SHL: 
Shal_2088
SWD: 
Swoo_1390
PHA: 
PSHAa2143(mhpE)
MHC: 
MARHY0649
MAD: 
HP15_1115 HP15_4036
TTU: 
TERTU_1283(dmpG)
LPN: 
lpg2681
LPU: 
LPE509_00348
LPF: 
lpl2608
LPP: 
lpp2735
LPC: 
LPC_0455
LPA: 
lpa_03915
LPE: 
lp12_2674
LLO: 
LLO_0366
TCY: 
Thicy_0923
MEJ: 
Q7A_1101
CYQ: 
Q91_0510 Q91_1491(lapG)
ALV: 
Alvin_2162
TVI: 
Thivi_0520
TMB: 
Thimo_1223
AEH: 
Mlg_2462
MMW: 
Mmwyl1_3801
MPC: 
Mar181_0890
GPB: 
HDN1F_27070(tesG)
RSO: 
RS01666(RSp0895)
RPI: 
Rpic_4617
RPF: 
Rpic12D_3541
REU: 
Reut_B5823
REH: 
H16_A1807(mhpE2) H16_B0552(mhpE1) H16_B0595(bpHI)
RME: 
Rmet_1319(bphI) Rmet_5209(mhpE)
CTI: 
RALTA_B0399(mhpE)
CNC: 
CNE_1c17880 CNE_2c04980(mhpE) CNE_2c05400(bpHI) CNE_BB1p11950 CNE_BB1p13240
BPS: 
BPSS1807(mhpE)
BPM: 
BURPS1710b_A0890(mhpE)
BPL: 
BURPS1106A_A2454
BPD: 
BURPS668_A2590(mhpE)
BPZ: 
BP1026B_II1937(mhpE)
BPQ: 
BPC006_II2418
BTE: 
BTH_II0570(mhpE)
BVI: 
Bcep1808_5384 Bcep1808_7605
BUR: 
Bcep18194_B1188 Bcep18194_B1457 Bcep18194_B2959 Bcep18194_C7644
BCJ: 
BCAM1615(bphI) BCAM2121
BCT: 
GEM_4109 GEM_5401
BXE: 
Bxe_A1152(amnG) Bxe_A3546 Bxe_B2325 Bxe_C1187(bphI)
BPH: 
Bphy_3510 Bphy_4539
BPY: 
Bphyt_1468
BGL: 
bglu_2g02770 bglu_2g05720
BUG: 
BC1001_0836
BGE: 
BC1002_4113 BC1002_4720
BRH: 
RBRH_00203
BGD: 
bgla_2g03170
BUK: 
MYA_5002
BPX: 
BUPH_04925 BUPH_05357 BUPH_08191
BAV: 
BAV0600(bphF)
AXY: 
AXYL_04307(dmpG)
PUT: 
PT7_2460
RFR: 
Rfer_0454
POL: 
Bpro_5136
PNA: 
Pnap_4142
AJS: 
Ajs_0224
ACK: 
C380_15915(bphF)
VEI: 
Veis_2781
CTT: 
CtCNB1_1351 CtCNB1_3147
ADN: 
Alide_0339
ADK: 
Alide2_0284
MPT: 
Mpe_A2266 Mpe_A3321
LCH: 
Lcho_3340
AZO: 
azo1857(lapG) azo1972(mhpE) azo2431(nahM)
DAR: 
Daro_0907 Daro_1356 Daro_3238 Daro_3782 Daro_3808
TMZ: 
Tmz1t_2079 Tmz1t_3105
CJD: 
JJD26997_0727
ABU: 
Abu_1489
ABT: 
ABED_1386
ANT: 
Arnit_0945
ARC: 
ABLL_1976 ABLL_2442
SUN: 
SUN_2142
GLO: 
Glov_0787
DMA: 
DMR_24610(nahM)
DPI: 
BN4_11362(nahM)
HOH: 
Hoch_1083
PEL: 
SAR11G3_00085
ARA: 
Arad_7255
BTR: 
Btr_2080
XAU: 
Xaut_0938
MSL: 
Msil_1479
OAR: 
OA238_c24310
NAR: 
Saro_3687 Saro_3852
NPP: 
PP1Y_AT15711 PP1Y_AT31574 PP1Y_AT32722 PP1Y_Mpl3118
SWI: 
Swit_1641 Swit_2113 Swit_4923
SPHM: 
G432_01975
SJP: 
SJA_C1-11910(mhpE_xylK)
SCH: 
Sphch_3493
TMO: 
TMO_3169(mhpE) TMO_b0123
PGV: 
SL003B_p0059
BSS: 
BSUW23_00995(rhiG)
BCA: 
BCE_2157
BCX: 
BCA_2155(dmpG)
BCF: 
bcf_10215
BCER: 
BCK_24220
BTL: 
BALH_1844
BPF: 
BpOF4_12610
BMQ: 
BMQ_1728 BMQ_3587(dmpG)
BMD: 
BMD_1715 BMD_3572(dmpG)
BMH: 
BMWSH_3486
GTN: 
GTNG_3151(nbaI)
GTH: 
Geoth_1679 Geoth_1949
GYC: 
GYMC61_2298
GYA: 
GYMC52_1426
GCT: 
GC56T3_2055
GMC: 
GY4MC1_1596 GY4MC1_1884
GGH: 
GHH_c14840(pheE)
BBE: 
BBR47_29780
PJD: 
Pjdr2_1367
GYM: 
GYMC10_5131
PMS: 
KNP414_00502 KNP414_04361
PMQ: 
PM3016_446
PMW: 
B2K_02290
TCO: 
Theco_1039
AAC: 
Aaci_1619
AAD: 
TC41_0802(mhpE) TC41_1515(mhpE)
BTS: 
Btus_0969
SIV: 
SSIL_0066(nbaI) SSIL_3690
EFC: 
EFAU004_01964
CSO: 
CLS_02690
CLB: 
Clo1100_2806
CCL: 
Clocl_3102
DSY: 
DSY3306
DHD: 
Dhaf_1245
DCA: 
Desca_1927
DKU: 
Desku_0617
PTH: 
PTH_0483(LeuA)
DOR: 
Desor_0613 Desor_5186
DED: 
DHBDCA_p524
DEC: 
DCF50_p582
EEL: 
EUBELI_01913
ERE: 
EUBREC_1320
ERT: 
EUR_08580 EUR_22860
ERA: 
ERE_05890 ERE_18390
ESR: 
ES1_22750
ESU: 
EUS_17010
BFI: 
CIY_16940 CIY_27770
CLE: 
Clole_1714
COO: 
CCU_13650
RAL: 
Rumal_0376
RBR: 
RBR_04690
TMR: 
Tmar_1781
SAY: 
TPY_0642(mhpE)
SAP: 
Sulac_2997
BPRS: 
CK3_33700
CHY: 
CHY_1280(mhpE)
MTA: 
Moth_1775
TOC: 
Toce_0378
SSG: 
Selsp_0535
MTU: 
Rv3469c(mhpE) Rv3534c(hsaF)
MTV: 
RVBD_3469c RVBD_3534c
MTC: 
MT3575(bphI-1) MT3638(bphI-2)
MRA: 
MRA_3509(mhpE) MRA_3573(bphI)
MTF: 
TBFG_13506 TBFG_13567
MTB: 
TBMG_03517 TBMG_03574
MTK: 
TBSG_03539 TBSG_03600
MTZ: 
TBXG_003488 TBXG_003549
MTG: 
MRGA327_21385 MRGA327_21835
MTE: 
CCDC5079_3211 CCDC5079_3275
MTL: 
CCDC5180_3165 CCDC5180_3227
MTO: 
MTCTRI2_3537(mhpE) MTCTRI2_3598
MTD: 
UDA_3469c(mhpE) UDA_3534c
MTN: 
ERDMAN_3810(mhpE) ERDMAN_3878
MTJ: 
J112_18655 J112_19030
MTUB: 
MT7199_3532 MT7199_3596(hsaF)
MBO: 
Mb3498c(mhpE) Mb3564c
MBB: 
BCG_3534c(mhpE) BCG_3598c
MBT: 
JTY_3534(mhpE) JTY_3599
MBM: 
BCGMEX_3532c(mhpE) BCGMEX_3596c
MBK: 
K60_036050 K60_036720
MAF: 
MAF_34840(mhpE) MAF_35460
MCE: 
MCAN_34851(mhpE) MCAN_35451
MCQ: 
BN44_110026(hsaF) BN44_80145
MCV: 
BN43_70136 BN43_90032(hsaF)
MCX: 
BN42_50147 BN42_90029
MCZ: 
BN45_100029(hsaF) BN45_70127
MPA: 
MAP0533
MAV: 
MAV_0627
MIT: 
OCO_05370 OCO_14610
MIR: 
OCQ_05520
MIA: 
OCU_05410 OCU_15050
MID: 
MIP_00991
MSM: 
MSMEG_4150 MSMEG_5937
MSG: 
MSMEI_4052 MSMEI_5778(bphI-2)
MSA: 
Mycsm_01981 Mycsm_05771
MUL: 
MUL_2435 MUL_4095
MVA: 
Mvan_0597 Mvan_4391 Mvan_5234
MGI: 
Mflv_1522
MSP: 
Mspyr1_09060
MAB: 
MAB_0626 MAB_4375
MMV: 
MYCMA_0326 MYCMA_2429
MMC: 
Mmcs_4659 Mmcs_5438
MKM: 
Mkms_4747 Mkms_5835
MJL: 
Mjls_4224 Mjls_5042
MJD: 
JDM601_3679
MMI: 
MMAR_1506 MMAR_5021
MRH: 
MycrhN_2296
MMM: 
W7S_02630
MCB: 
Mycch_1595 Mycch_4599
MLI: 
MULP_01624 MULP_05273
ASD: 
AS9A_0172 AS9A_1020
CDR: 
CDHC03_1949(mhpE)
NFA: 
nfa30510 nfa33200 nfa4670
NCY: 
NOCYR_0495(mhpE)
NBR: 
O3I_002630
RHA: 
RHA1_ro00515 RHA1_ro03867 RHA1_ro04535(hsaF) RHA1_ro05801 RHA1_ro08083(bphF3) RHA1_ro09019(bphF1) RHA1_ro10112(bphF4)
RER: 
RER_07410 RER_57580
ROP: 
ROP_34160(cmtG) ROP_37360 ROP_44500 ROP_54050 ROP_58620 ROP_pROB02-02240
REQ: 
REQ_42060
GBR: 
Gbro_0238 Gbro_0911
GPO: 
GPOL_c41170(hsaF)
GOR: 
KTR9_0812(hsaF)
TPR: 
Tpau_3800
SCO: 
SCP1.301 SCP1.53c
SGR: 
SGR_564
SCB: 
SCAB_15191 SCAB_2071(mhpE)
SSX: 
SACTE_4114
SVL: 
Strvi_3232
SBH: 
SBI_01535 SBI_09086
SDV: 
BN159_1388 BN159_4098
KSK: 
KSE_23710
ICA: 
Intca_1094
NCA: 
Noca_1649 Noca_2149
NDA: 
Ndas_2258
TCU: 
Tcur_0536 Tcur_3470
SRO: 
Sros_9113
FRE: 
Franean1_2882 Franean1_4743
FRI: 
FraEuI1c_4108 FraEuI1c_5353
FAL: 
FRAAL4663
FSY: 
FsymDg_2038
NML: 
Namu_4081
AMD: 
AMED_4220(mhpE)
AMN: 
RAM_21495
AMM: 
AMES_4170(mhpE)
PDX: 
Psed_4179
STP: 
Strop_3525
SAQ: 
Sare_3900 Sare_4567
MAU: 
Micau_4938
MIL: 
ML5_3357
VMA: 
VAB18032_17785 VAB18032_28746
AMS: 
AMIS_19100
CWO: 
Cwoe_0441 Cwoe_4743 Cwoe_4833
TBE: 
Trebr_0812
TPI: 
TREPR_1324(dmpG)
SSM: 
Spirs_4175
BRM: 
Bmur_0128
BIP: 
Bint_1446
BVU: 
BVU_2661 BVU_4036
BSA: 
Bacsa_0074
PPN: 
Palpr_1681
PTQ: 
P700755_001636
NDO: 
DDD_3546
POM: 
MED152_05635
FSU: 
Fisuc_2032
FSC: 
FSU_2557
ACO: 
Amico_0146
SYW: 
SYNW0424
SYNE: 
Syn6312_0744
ARP: 
NIES39_A00060
CTS: 
Ctha_2137
RRS: 
RoseRS_2573
RCA: 
Rcas_2410
CAU: 
Caur_1351
CAG: 
Cagg_2034
CHL: 
Chy400_1473
TRO: 
trd_1392
CAP: 
CLDAP_40960
TTJ: 
TTHB246
TTL: 
TtJL18_2427
TSC: 
TSC_c11570(dmpG)
THC: 
TCCBUS3UF1_13060
TOS: 
Theos_2473
MLA: 
Mlab_0987
TAR: 
TALC_00100
NMR: 
Nmar_0140
 » show all
Taxonomy
Reference
1  [PMID:12764229]
  Authors
Manjasetty BA, Powlowski J, Vrielink A.
  Title
Crystal structure of a bifunctional aldolase-dehydrogenase: sequestering a reactive and volatile intermediate.
  Journal
Proc. Natl. Acad. Sci. U. S. A. 100 (2003) 6992-7.
  Organism
Pseudomonas sp.
Reference
2  [PMID:8419288]
  Authors
Powlowski J, Sahlman L, Shingler V.
  Title
Purification and properties of the physically associated meta-cleavage pathway enzymes 4-hydroxy-2-ketovalerate aldolase and aldehyde dehydrogenase (acylating) from Pseudomonas sp. strain CF600.
  Journal
J. Bacteriol. 175 (1993) 377-85.
  Organism
Pseudomonas sp.
Reference
3  [PMID:11264589]
  Authors
Manjasetty BA, Croteau N, Powlowski J, Vrielink A.
  Title
Crystallization and preliminary X-ray analysis of dmpFG-encoded 4-hydroxy-2-ketovalerate aldolase--aldehyde dehydrogenase (acylating) from Pseudomonas sp. strain CF600.
  Journal
Acta. Crystallogr. D. Biol. Crystallogr. 57 (2001) 582-5.
  Organism
Pseudomonas sp.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
UM-BBD (Biocatalysis/Biodegradation Database): 
BRENDA, the Enzyme Database: 
CAS: 
37325-52-3
DBGET integrated database retrieval system