KEGG   ENZYME: 4.2.1.36Help
Entry
EC 4.2.1.36                 Enzyme                                 

Name
homoaconitate hydratase;
homoaconitase;
cis-homoaconitase;
HACN;
Lys4;
LysF;
2-hydroxybutane-1,2,4-tricarboxylate hydro-lyase (incorrect)
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
BRITE hierarchy
Sysname
(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate hydro-lyase [(Z)-but-1-ene-1,2,4-tricarboxylate-forming]
Reaction(IUBMB)
(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate = (Z)-but-1-ene-1,2,4-tricarboxylate + H2O [RN:R04371]
Reaction(KEGG)
Substrate
(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate [CPD:C05662]
Product
(Z)-but-1-ene-1,2,4-tricarboxylate [CPD:C04002];
H2O [CPD:C00001]
Comment
Requires a [4Fe-4S] cluster for activity. The enzyme from the hyperthermophilic eubacterium Thermus thermophilus can catalyse the reaction shown above but cannot catalyse the previously described reaction, i.e. formation of (R)-homocitrate by hydration of cis-homoaconitate. The enzyme responsible for the conversion of cis-homoaconitate into (R)-homocitrate in T. thermophilus is unknown at present but the reaction can be catalysed in vitro using aconitate hydratase from pig (EC 4.2.1.3) [2].
History
EC 4.2.1.36 created 1972, modified 2007
Pathway
ec00300  Lysine biosynthesis
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
ec01130  Biosynthesis of antibiotics
Orthology
K01705  homoaconitate hydratase
Genes
SCE: YDR234W(LYS4)
AGO: AGOS_ABL106C
ERC: Ecym_5123
KLA: KLLA0C15125g
KMX: KLMA_30527(LYS4)
LTH: KLTH0E10582g
VPO: Kpol_1031p57
ZRO: ZYRO0A10076g
CGR: CAGL0K10978g
NCS: NCAS_0B02660(NCAS0B02660)
NDI: NDAI_0C05030(NDAI0C05030)
TPF: TPHA_0L01150(TPHA0L01150)
TBL: TBLA_0A01460(TBLA0A01460)
TDL: TDEL_0B01800(TDEL0B01800)
KAF: KAFR_0E02730(KAFR0E02730)
PIC: PICST_88705(LYS4)
SPAA: SPAPADRAFT_58782(LYS4)
CAL: CAALFM_C404410CA(LYS4)
SLB: AWJ20_348(LYS4)
NCR: NCU08898(lys-6)
NTE: NEUTE1DRAFT58933(NEUTE1DRAFT_58933)
MGR: MGG_04842
CMT: CCM_07793
BFU: BCIN_14g00430(Bclys4)
MBE: MBM_05638
ANI: AN6521.2
ANG: ANI_1_1106134(An15g00350)
ABE: ARB_04155
TVE: TRV_03936
PNO: SNOG_01131(SNOG_01129 SNOG_01130)
PTE: PTT_19520
SPO: SPAC343.16(lys2)
CNE: CNK00580
CNB: CNBK2910
ABP: AGABI1DRAFT59676(AGABI1DRAFT_59676)
ABV: AGABI2DRAFT184036(AGABI2DRAFT_184036)
MGL: MGL_3298
SPAR: SPRG_03993
IAL: IALB_1150
MRO: MROS_2220
CABY: Cabys_582
 » show all
Taxonomy
Reference
1  [PMID:5954805]
  Authors
Strassman M, Ceci LN.
  Title
Enzymatic formation of cis-homoaconitic acid, an intermediate in lysine biosynthesis in yeast.
  Journal
J. Biol. Chem. 241 (1966) 5401-7.
Reference
2  [PMID:16524361]
  Authors
Jia Y, Tomita T, Yamauchi K, Nishiyama M, Palmer DR.
  Title
Kinetics and product analysis of the reaction catalysed by recombinant homoaconitase from Thermus thermophilus.
  Journal
Biochem. J. 396 (2006) 479-85.
Reference
3  [PMID:10714900]
  Authors
Zabriskie TM, Jackson MD.
  Title
Lysine biosynthesis and metabolism in fungi.
  Journal
Nat. Prod. Rep. 17 (2000) 85-97.
Other DBs
ExplorEnz - The Enzyme Database: 4.2.1.36
IUBMB Enzyme Nomenclature: 4.2.1.36
ExPASy - ENZYME nomenclature database: 4.2.1.36
BRENDA, the Enzyme Database: 4.2.1.36
CAS: 9030-68-6

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