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Entry
EC 4.4.1.28                 Enzyme                                 

Name
L-cysteine desulfidase;
L-cysteine desulfhydrase
Class
Lyases;
Carbon-sulfur lyases;
Carbon-sulfur lyases (only sub-subclass identified to date)
BRITE hierarchy
Sysname
L-cysteine sulfide-lyase (deaminating; pyruvate-forming)
Reaction(IUBMB)
L-cysteine + H2O = sulfide + NH3 + pyruvate (overall reaction) [RN:R00782];
(1a) L-cysteine = 2-aminoprop-2-enoate + sulfide;
(1b) 2-aminoprop-2-enoate = 2-iminopropanoate (spontaneous);
(1c) 2-iminopropanoate + H2O = pyruvate + NH3 (spontaneous)
Reaction(KEGG)
Substrate
L-cysteine [CPD:C00097];
H2O [CPD:C00001];
2-aminoprop-2-enoate [CPD:C02218];
2-iminopropanoate [CPD:C20904]
Product
sulfide [CPD:C00283];
NH3 [CPD:C00014];
pyruvate [CPD:C00022];
2-aminoprop-2-enoate [CPD:C02218];
2-iminopropanoate [CPD:C20904]
Comment
The enzyme from the archaeon Methanocaldococcus jannaschii contains a [4Fe-4S] cluster and is specific for L-cysteine (cf. EC 4.4.1.1, cystathionine gamma-lyase). It cleaves a carbon-sulfur bond releasing sulfide and the unstable enamine product 2-aminoprop-2-enoate that tautomerizes to an imine form, which undergoes a hydrolytic deamination to form pyruvate and ammonia. The same reaction can also be catalysed by some pyridoxal-phosphate proteins (cf. EC 4.4.1.1, cystathionine gamma-lyase).
History
EC 4.4.1.28 created 2014
Pathway
Cysteine and methionine metabolism
Orthology
K20021  
L-cysteine desulfidase
K22207  
L-cysteine desulfhydrase
Genes
RSS: 
AQU: 
ATH: 
ALY: 
CRB: 
CSAT: 
EUS: 
BRP: 
BNA: 
BOE: 
THJ: 
CPAP: 
CIT: 
CIC: 
TCC: 
GRA: 
GHI: 
EGR: 
GMX: 
PVU: 
VRA: 
VAR: 
CCAJ: 
MTR: 
CAM: 
ADU: 
AIP: 
LJA: 
Lj0g3v0131109.1(Lj0g3v0131109.1) Lj0g3v0330309.1(Lj0g3v0330309.1) Lj1g3v4807370.1(Lj1g3v4807370.1)
LANG: 
FVE: 
PPER: 
PMUM: 
PAVI: 
MDM: 
PXB: 
ZJU: 
CSV: 
CMO: 
MCHA: 
RCU: 
JCU: 
HBR: 
POP: 
POPTR_0002s18830g(POPTRDRAFT_1072696) POPTR_0005s00480g POPTR_0013s00400g(POPTRDRAFT_570656) POPTR_0014s10830g(POPTRDRAFT_572528)
VVI: 
SLY: 
SPEN: 
SOT: 
CANN: 
NTA: 
NSY: 
NTO: 
INI: 
SIND: 
HAN: 
BVG: 
SOE: 
NNU: 
OSA: 
DOSA: 
Os01t0290100-01(Os01g0290100) Os01t0290600-00(Os01g0290600)
OBR: 
BDI: 
ATS: 
109769734(LOC109769734) 109784901(LOC109784901)
SBI: 
ZMA: 
SITA: 
PDA: 
EGU: 
MUS: 
DCT: 
ATR: 
SMO: 
PPP: 
CRE: 
MIS: 
SRE: 
SMIN: 
v1.2.005268.t1(symbB.v1.2.005268.t1) v1.2.005268.t2(symbB.v1.2.005268.t2)
AAF: 
SPAR: 
MJA: 
MFE: 
MVU: 
MFS: 
MIF: 
MJH: 
MIG: 
MMP: 
MMQ: 
MMX: 
MMZ: 
MMD: 
MMAK: 
MMAO: 
 » show all
Taxonomy
Reference
1  [PMID:15683250]
  Authors
Tchong SI, Xu H, White RH
  Title
L-cysteine desulfidase: an [4Fe-4S] enzyme isolated from Methanocaldococcus jannaschii that catalyzes the breakdown of L-cysteine into pyruvate, ammonia, and sulfide.
  Journal
Biochemistry. 44 (2005) 1659-70.
  Sequence
[mja:MJ_1025]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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